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Literature summary extracted from

  • Zarzycki, J.; Brecht, V.; Muller, M.; Fuchs, G.
    Identifying the missing steps of the autotrophic 3-hydroxypropionate CO2 fixation cycle in Chloroflexus aurantiacus (2009), Proc. Natl. Acad. Sci. USA, 106, 21317-21322.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.1.3 expressed in Escherichia coli as an N-terminal His10-tagged protein Chloroflexus aurantiacus

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.4.1.3 hydroxylamine
-
 Chloroflexus aurantiacus
5.4.1.3 additional information sodium borohydride has no effect Chloroflexus aurantiacus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.153 30000
-
 x * 30000, SDS-PAGE Chloroflexus aurantiacus
5.4.1.3 47000
-
 2 * 47000 Chloroflexus aurantiacus
5.4.1.3 73000
-
 gel filtration Chloroflexus aurantiacus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.153 mesaconyl-C4-CoA + H2O Chloroflexus aurantiacus
-
 (S)-citramalyl-CoA
-
 ?
5.4.1.3 2-methylfumaryl-CoA Chloroflexus aurantiacus the enzyme is part of the 3-hydroxypropanoate cycle for carbon assimilation 3-methylfumaryl-CoA
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.153 Chloroflexus aurantiacus A9WC41
-
-
5.4.1.3 Chloroflexus aurantiacus A9WC36
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.153
-
 Chloroflexus aurantiacus
5.4.1.3
-
 Chloroflexus aurantiacus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.153 mesaconyl-C4-CoA + H2O
-
 Chloroflexus aurantiacus (S)-citramalyl-CoA
-
 ?
5.4.1.3 2-methylfumaryl-CoA the enzyme is part of the 3-hydroxypropanoate cycle for carbon assimilation Chloroflexus aurantiacus 3-methylfumaryl-CoA
-
 r
5.4.1.3 2-methylfumaryl-CoA 2-methylfumaryl-CoA i.e. (E)-3-carboxy-2-methylprop-2-enoyl-CoA. The enzyme acts as an intramolecular CoA transferase and does not transfer CoA to free mesaconate Chloroflexus aurantiacus 3-methylfumaryl-CoA 3-methylfumaryl-CoA i.e. (E)-3-carboxybut-2-enoyl-CoA r

Subunits

EC Number Subunits Comment Organism
4.2.1.153 ? x * 30000, SDS-PAGE Chloroflexus aurantiacus
5.4.1.3 homodimer 2 * 47000 Chloroflexus aurantiacus

Synonyms

EC Number Synonyms Comment Organism
4.2.1.153 mEH
-
 Chloroflexus aurantiacus
4.2.1.153 mesaconyl-C4-CoA hydratase
-
 Chloroflexus aurantiacus
5.4.1.3 MCT
-
 Chloroflexus aurantiacus
5.4.1.3 mesaconyl-CoA C1-C4 CoA transferase
-
 Chloroflexus aurantiacus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.4.1.3 55
-
 assay at Chloroflexus aurantiacus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.4.1.3 840
-
 2-methylfumaryl-CoA 55°C, pH not specified in the publication Chloroflexus aurantiacus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4.1.3 7.5 7.8 at 20°C Chloroflexus aurantiacus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.4.1.3 6.6
-
 at 20°C, 50% of maximal activity Chloroflexus aurantiacus

General Information

EC Number General Information Comment Organism
5.4.1.3 physiological function the enzyme is part of the 3-hydroxypropanoate cycle for carbon assimilation Chloroflexus aurantiacus