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Literature summary extracted from

  • Hiblot, J.; Gotthard, G.; Elias, M.; Chabriere, E.
    Differential active site loop conformations mediate promiscuous activities in the lactonase SsoPox (2013), PLoS One, 8, e75272.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.25 hanging drop vapor diffusion method, in order to understand the structural consequences of variations at position 263, the crystal structures of the mutant enzymes (W263F, W263M, W263L, W263I, W263V, W263T) is solved Saccharolobus solfataricus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.25 W263F mutant exhibits a lower Tm-value compared to the wild-type enzyme. Catalytic efficiency (kcat/Km) of paraoxon increases 23.3fold compared to wild-type value. 1.3fold increase in catalytic efficiency (kcat/Km) of N-(3-oxododecanoyl)-L-homoserine lactone Saccharolobus solfataricus
3.1.1.25 W263I mutant exhibits a lower Tm-value compared to the wild-type enzyme. 45.5fold increase in catalytic efficiency (kcat/Km) of N-(3-oxododecanoyl)-L-homoserine lactone Saccharolobus solfataricus
3.1.1.25 W263L mutant exhibits a lower Tm-value compared to the wild-type enzyme. Catalytic efficiency (kcat/Km) of paraoxon increases 4.6fold compared to wild-type value Saccharolobus solfataricus
3.1.1.25 W263M mutant exhibits a lower Tm-value compared to the wild-type enzyme. Catalytic efficiency (kcat/Km) of paraoxon increases 14.1fold compared to wild-type value Saccharolobus solfataricus
3.1.1.25 W263T mutant exhibits a lower Tm-value compared to the wild-type enzyme. 21.2fold increase in catalytic efficiency (kcat/Km) of N-(3-oxododecanoyl)-L-homoserine lactone Saccharolobus solfataricus
3.1.1.25 W263V mutant exhibits a lower Tm-value compared to the wild-type enzyme. 54.5fold increase in catalytic efficiency (kcat/Km) of N-(3-oxododecanoyl)-L-homoserine lactone Saccharolobus solfataricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.25 delta-undecanolactone contrary to the wild-type enzyme and mutant enzyme W263F, the mutant enzymes W263M, W263L, W263I, W263V and W263T exhibit a substrate inhibition Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.25 0.013
-
 gamma-undecanolactone pH 8.3, 25°C, mutant enzyme W263T Saccharolobus solfataricus
3.1.1.25 0.0178
-
 N-(3-oxododecanoyl)-L-homoserine lactone pH 8.3, 25°C, mutant enzyme W263I Saccharolobus solfataricus
3.1.1.25 0.0247
-
 N-(3-oxododecanoyl)-L-homoserine lactone pH 8.3, 25°C, mutant enzyme W263V Saccharolobus solfataricus
3.1.1.25 0.057
-
 delta-undecanolactone pH 8.3, 25°C, mutant enzyme W263V Saccharolobus solfataricus
3.1.1.25 0.094
-
 delta-undecanolactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 0.123
-
 N-(3-oxooctanoyl)-L-homoserine lactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 0.13
-
 delta-undecanolactone pH 8.3, 25°C, mutant enzyme W263T Saccharolobus solfataricus
3.1.1.25 0.135
-
 delta-undecanolactone pH 8.3, 25°C, mutant enzyme W263F Saccharolobus solfataricus
3.1.1.25 0.137
-
 N-(3-oxododecanoyl)-L-homoserine lactone pH 8.3, 25°C, mutant enzyme W263T Saccharolobus solfataricus
3.1.1.25 0.143
-
 N-(3-oxodecanoyl)-L-homoserine lactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 0.145
-
 N-octanoylhomoserine lactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 0.146
-
 N-(3-oxododecanoyl)-L-homoserine lactone pH 8.3, 25°C, mutant enzyme W263F Saccharolobus solfataricus
3.1.1.25 0.161
-
 delta-undecanolactone pH 8.3, 25°C, mutant enzyme W263M Saccharolobus solfataricus
3.1.1.25 0.166
-
 gamma-heptanolactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 0.219
-
 delta-undecanolactone pH 8.3, 25°C, mutant enzyme W263L Saccharolobus solfataricus
3.1.1.25 0.234
-
 epsilon-caprolactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 0.256
-
 N-(3-oxooctanoyl)homoserine lactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 0.288
-
 N-(3-oxodecanoyl)-L-homoserine lactone pH 8.3, 25°C, mutant enzyme W263F Saccharolobus solfataricus
3.1.1.25 0.334
-
 gamma-undecanolactone pH 8.3, 25°C, mutant enzyme W263M Saccharolobus solfataricus
3.1.1.25 0.345
-
 N-dodecanoylhomoserine lactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 0.359
-
 delta-nonalactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 0.361
-
 gamma-undecanolactone pH 8.3, 25°C, mutant enzyme W263I Saccharolobus solfataricus
3.1.1.25 0.372
-
 gamma-undecanolactone pH 8.3, 25°C, mutant enzyme W263L Saccharolobus solfataricus
3.1.1.25 0.373
-
 gamma-undecanolactone pH 8.3, 25°C, mutant enzyme W263F Saccharolobus solfataricus
3.1.1.25 0.456
-
 N-(3-oxododecanoyl)-L-homoserine lactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 0.459
-
 N-hexanoylhomoserine lactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 0.558
-
 N-(3-oxohexanoyl)-L-homoserine lactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 0.592
-
 N-(3-oxohexanoyl)homoserine lactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 0.7
-
 paraoxon pH 8.3, 25°C, mutant enzyme W263F Saccharolobus solfataricus
3.1.1.25 0.93
-
 paraoxon pH 8.3, 25°C, mutant enzyme W263M Saccharolobus solfataricus
3.1.1.25 1
-
 N-(3-oxodecanoyl)-L-homoserine lactone pH 8.3, 25°C, mutant enzyme W263T Saccharolobus solfataricus
3.1.1.25 1.22
-
 gamma-dodecanolactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 1.346
-
 N-(3-oxodecanoyl)-L-homoserine lactone pH 8.3, 25°C, mutant enzyme W263V Saccharolobus solfataricus
3.1.1.25 1.376
-
 dihydrocoumarin pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 1.605
-
 N-(3-oxodecanoyl)-L-homoserine lactone pH 8.3, 25°C, mutant enzyme W263I Saccharolobus solfataricus
3.1.1.25 1.678
-
 delta-dodecanolactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 1.76
-
 gamma-undecanolactone pH 8.3, 25°C, mutant enzyme W263V Saccharolobus solfataricus
3.1.1.25 2.099
-
 gamma-undecanolactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 2.943
-
 gamma-nonalactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 24.2
-
 paraoxon pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.25 N-(3-oxodecanoyl)-L-homoserine lactone + H2O Saccharolobus solfataricus the best, possibly natural, substrate of wild-type enzyme N-(3-oxodecanoyl)-L-homoserine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.25 Saccharolobus solfataricus Q97VT7
-
-
3.1.1.25 Saccharolobus solfataricus P2 Q97VT7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.25
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.25 delta-dodecanolactone + H2O
-
 Saccharolobus solfataricus 5-hydroxydodecanoate
-
 ?
3.1.1.25 delta-nonalactone + H2O
-
 Saccharolobus solfataricus 5-hydroxynonanoate
-
 ?
3.1.1.25 delta-undecanolactone + H2O
-
 Saccharolobus solfataricus 5-hydroxyundecanoate
-
 ?
3.1.1.25 dihydrocoumarin + H2O
-
 Saccharolobus solfataricus 3-(2-hydroxyphenyl)-propionic acid
-
 ?
3.1.1.25 dihydrocoumarin + H2O
-
 Saccharolobus solfataricus P2 3-(2-hydroxyphenyl)-propionic acid
-
 ?
3.1.1.25 epsilon-caprolactone + H2O
-
 Saccharolobus solfataricus ?
-
 ?
3.1.1.25 epsilon-caprolactone + H2O
-
 Saccharolobus solfataricus P2 ?
-
 ?
3.1.1.25 gamma-butyrolactone + H2O
-
 Saccharolobus solfataricus 4-hydroxybutanoate
-
 ?
3.1.1.25 gamma-dodecanolactone + H2O
-
 Saccharolobus solfataricus 4-hydroxydodecanoate
-
 ?
3.1.1.25 gamma-heptanolactone + H2O
-
 Saccharolobus solfataricus 4-hydroxyheptanoate
-
 ?
3.1.1.25 gamma-nonalactone + H2O
-
 Saccharolobus solfataricus 4-hydroxynonanoate
-
 ?
3.1.1.25 gamma-undecanolactone + H2O
-
 Saccharolobus solfataricus 4-hydroxyundecanoate
-
 ?
3.1.1.25 N-(3-oxodecanoyl)-L-homoserine lactone + H2O
-
 Saccharolobus solfataricus N-(3-oxodecanoyl)-L-homoserine
-
 ?
3.1.1.25 N-(3-oxodecanoyl)-L-homoserine lactone + H2O the best, possibly natural, substrate of wild-type enzyme Saccharolobus solfataricus N-(3-oxodecanoyl)-L-homoserine
-
 ?
3.1.1.25 N-(3-oxododecanoyl)-L-homoserine lactone + H2O
-
 Saccharolobus solfataricus N-(3-oxododecanoyl)-L-homoserine
-
 ?
3.1.1.25 N-(3-oxohexanoyl)-L-homoserine lactone + H2O
-
 Saccharolobus solfataricus N-(3-oxohexanoyl)-L-homoserine
-
 ?
3.1.1.25 N-(3-oxohexanoyl)-L-homoserine lactone + H2O
-
 Saccharolobus solfataricus P2 N-(3-oxohexanoyl)-L-homoserine
-
 ?
3.1.1.25 N-(3-oxohexanoyl)homoserine lactone + H2O
-
 Saccharolobus solfataricus N-(3-oxohexanoyl)homoserine
-
 ?
3.1.1.25 N-(3-oxooctanoyl)-L-homoserine lactone + H2O
-
 Saccharolobus solfataricus N-(3-oxooctanoyl)-L-homoserine
-
 ?
3.1.1.25 N-(3-oxooctanoyl)-L-homoserine lactone + H2O
-
 Saccharolobus solfataricus P2 N-(3-oxooctanoyl)-L-homoserine
-
 ?
3.1.1.25 N-(3-oxooctanoyl)homoserine lactone + H2O
-
 Saccharolobus solfataricus N-(3-oxooctanoyl)homoserine
-
 ?
3.1.1.25 N-butanoylhomoserine lactone + H2O
-
 Saccharolobus solfataricus N-butanoylhomoserine
-
 ?
3.1.1.25 N-dodecanoylhomoserine lactone + H2O
-
 Saccharolobus solfataricus N-dodecanoylhomoserine
-
 ?
3.1.1.25 N-hexanoylhomoserine lactone + H2O
-
 Saccharolobus solfataricus N-hexanoylhomoserine
-
 ?
3.1.1.25 N-octanoylhomoserine lactone + H2O
-
 Saccharolobus solfataricus N-octanoylhomoserine
-
 ?
3.1.1.25 paraoxon + H2O
-
 Saccharolobus solfataricus diethylphosphate + 4-nitrophenol
-
 ?
3.1.1.25 paraoxon + H2O
-
 Saccharolobus solfataricus P2 diethylphosphate + 4-nitrophenol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.25 SSO2522 locus name Saccharolobus solfataricus
3.1.1.25 SsoPox
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.25 25
-
 assay at Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.25 additional information
-
 mutant enzymes (W263F, W263M, W263L, W263I, W263V, W263T) exhibit a lower Tm-value compared to the wild-type enzyme Saccharolobus solfataricus
3.1.1.25 84
-
 Tm-value of mutant enzyme W263L Saccharolobus solfataricus
3.1.1.25 92
-
 Tm-value of mutant enzyme W263L Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.25 0.041
-
 N-(3-oxohexanoyl)homoserine lactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 0.083
-
 N-(3-oxohexanoyl)-L-homoserine lactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 0.107
-
 N-(3-oxodecanoyl)-L-homoserine lactone pH 8.3, 25°C, mutant enzyme W263T Saccharolobus solfataricus
3.1.1.25 0.19
-
 N-(3-oxodecanoyl)-L-homoserine lactone pH 8.3, 25°C, mutant enzyme W263V Saccharolobus solfataricus
3.1.1.25 0.36
-
 N-hexanoylhomoserine lactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 0.41
-
 N-(3-oxododecanoyl)-L-homoserine lactone pH 8.3, 25°C, mutant enzyme W263F Saccharolobus solfataricus
3.1.1.25 0.42
-
 N-(3-oxooctanoyl)homoserine lactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 0.54
-
 N-(3-oxooctanoyl)-L-homoserine lactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 0.6
-
 N-(3-oxodecanoyl)-L-homoserine lactone pH 8.3, 25°C, mutant enzyme W263I Saccharolobus solfataricus
3.1.1.25 1
-
 N-octanoylhomoserine lactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 1.01
-
 N-(3-oxododecanoyl)-L-homoserine lactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 1.7
-
 N-dodecanoylhomoserine lactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 1.8
-
 N-(3-oxododecanoyl)-L-homoserine lactone pH 8.3, 25°C, mutant enzyme W263I Saccharolobus solfataricus
3.1.1.25 1.94
-
 gamma-undecanolactone pH 8.3, 25°C, mutant enzyme W263I Saccharolobus solfataricus
3.1.1.25 2.72
-
 gamma-dodecanolactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 2.92
-
 gamma-heptanolactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 3
-
 N-(3-oxododecanoyl)-L-homoserine lactone pH 8.3, 25°C, mutant enzyme W263V Saccharolobus solfataricus
3.1.1.25 3.92
-
 gamma-undecanolactone pH 8.3, 25°C, mutant enzyme W263L Saccharolobus solfataricus
3.1.1.25 3.96
-
 N-(3-oxodecanoyl)-L-homoserine lactone pH 8.3, 25°C, mutant enzyme W263F Saccharolobus solfataricus
3.1.1.25 4.25
-
 gamma-undecanolactone pH 8.3, 25°C, mutant enzyme W263M Saccharolobus solfataricus
3.1.1.25 4.45
-
 epsilon-caprolactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 4.52
-
 N-(3-oxodecanoyl)-L-homoserine lactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 4.55
-
 gamma-undecanolactone pH 8.3, 25°C, mutant enzyme W263T Saccharolobus solfataricus
3.1.1.25 4.63
-
 gamma-undecanolactone pH 8.3, 25°C, mutant enzyme W263F Saccharolobus solfataricus
3.1.1.25 4.95
-
 gamma-undecanolactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 5.54
-
 gamma-nonalactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 5.64
-
 gamma-undecanolactone pH 8.3, 25°C, mutant enzyme W263V Saccharolobus solfataricus
3.1.1.25 6.44
-
 N-(3-oxododecanoyl)-L-homoserine lactone pH 8.3, 25°C, mutant enzyme W263T Saccharolobus solfataricus
3.1.1.25 6.82
-
 paraoxon pH 8.3, 25°C, mutant enzyme W263M Saccharolobus solfataricus
3.1.1.25 7.32
-
 dihydrocoumarin pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 7.38
-
 delta-undecanolactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 8.47
-
 paraoxon pH 8.3, 25°C, mutant enzyme W263F Saccharolobus solfataricus
3.1.1.25 12.6
-
 paraoxon pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 12.65
-
 delta-undecanolactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 15.32
-
 delta-nonalactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 44.8
-
 delta-undecanolactone pH 8.3, 25°C, mutant enzyme W263V Saccharolobus solfataricus
3.1.1.25 56.8
-
 delta-undecanolactone pH 8.3, 25°C, mutant enzyme W263L Saccharolobus solfataricus
3.1.1.25 58
-
 delta-undecanolactone pH 8.3, 25°C, mutant enzyme W263I Saccharolobus solfataricus
3.1.1.25 66.5
-
 delta-undecanolactone pH 8.3, 25°C, mutant enzyme W263F Saccharolobus solfataricus
3.1.1.25 71.2
-
 delta-undecanolactone pH 8.3, 25°C, mutant enzyme W263M Saccharolobus solfataricus
3.1.1.25 93.3
-
 delta-undecanolactone pH 8.3, 25°C, mutant enzyme W263T Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.25 8.3
-
 assay at Saccharolobus solfataricus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.1.25 0.789
-
 delta-undecanolactone pH 8.3, 25°C, mutant enzyme W263V Saccharolobus solfataricus
3.1.1.25 7.4
-
 delta-undecanolactone pH 8.3, 25°C, mutant enzyme W263M Saccharolobus solfataricus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.25 0.012
-
 N-butanoylhomoserine lactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 0.069
-
 N-(3-oxohexanoyl)homoserine lactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 0.106
-
 N-(3-oxodecanoyl)-L-homoserine lactone pH 8.3, 25°C, mutant enzyme W263T Saccharolobus solfataricus
3.1.1.25 0.141
-
 N-(3-oxodecanoyl)-L-homoserine lactone pH 8.3, 25°C, mutant enzyme W263V Saccharolobus solfataricus
3.1.1.25 0.15
-
 N-(3-oxohexanoyl)-L-homoserine lactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 0.374
-
 N-(3-oxodecanoyl)-L-homoserine lactone pH 8.3, 25°C, mutant enzyme W263I Saccharolobus solfataricus
3.1.1.25 0.519
-
 paraoxon pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 0.78
-
 N-hexanoylhomoserine lactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 0.883
-
 paraoxon pH 8.3, 25°C, mutant enzyme W263V Saccharolobus solfataricus
3.1.1.25 1.06
-
 paraoxon pH 8.3, 25°C, mutant enzyme W263T Saccharolobus solfataricus
3.1.1.25 1.2
-
 gamma-butyrolactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 1.21
-
 paraoxon pH 8.3, 25°C, mutant enzyme W263I Saccharolobus solfataricus
3.1.1.25 1.63
-
 N-(3-oxooctanoyl)homoserine lactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 1.88
-
 gamma-nonalactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 2.2
-
 N-(3-oxododecanoyl)-L-homoserine lactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 2.22
-
 N-(3-oxododecanoyl)-L-homoserine lactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 2.23
-
 gamma-dodecanolactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 2.36
-
 gamma-undecanolactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 2.37
-
 paraoxon pH 8.3, 25°C, mutant enzyme W263L Saccharolobus solfataricus
3.1.1.25 2.81
-
 N-(3-oxododecanoyl)-L-homoserine lactone pH 8.3, 25°C, mutant enzyme W263F Saccharolobus solfataricus
3.1.1.25 3.2
-
 gamma-undecanolactone pH 8.3, 25°C, mutant enzyme W263V Saccharolobus solfataricus
3.1.1.25 4.39
-
 N-(3-oxooctanoyl)-L-homoserine lactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 4.9
-
 N-dodecanoylhomoserine lactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 5.32
-
 dihydrocoumarin pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 5.37
-
 gamma-undecanolactone pH 8.3, 25°C, mutant enzyme W263I Saccharolobus solfataricus
3.1.1.25 6.9
-
 N-octanoylhomoserine lactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 7.33
-
 paraoxon pH 8.3, 25°C, mutant enzyme W263M Saccharolobus solfataricus
3.1.1.25 7.54
-
 delta-dodecanolactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 10.5
-
 gamma-undecanolactone pH 8.3, 25°C, mutant enzyme W263L Saccharolobus solfataricus
3.1.1.25 12.1
-
 paraoxon pH 8.3, 25°C, mutant enzyme W263F Saccharolobus solfataricus
3.1.1.25 12.4
-
 gamma-undecanolactone pH 8.3, 25°C, mutant enzyme W263F Saccharolobus solfataricus
3.1.1.25 12.7
-
 gamma-undecanolactone pH 8.3, 25°C, mutant enzyme W263M Saccharolobus solfataricus
3.1.1.25 13.8
-
 N-(3-oxodecanoyl)-L-homoserine lactone pH 8.3, 25°C, mutant enzyme W263F Saccharolobus solfataricus
3.1.1.25 17.6
-
 gamma-heptanolactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 19
-
 epsilon-caprolactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 31.6
-
 N-(3-oxodecanoyl)-L-homoserine lactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 42.7
-
 delta-nonalactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 47
-
 N-(3-oxododecanoyl)-L-homoserine lactone pH 8.3, 25°C, mutant enzyme W263T Saccharolobus solfataricus
3.1.1.25 78.6
-
 delta-undecanolactone pH 8.3, 25°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.25 101
-
 N-(3-oxododecanoyl)-L-homoserine lactone pH 8.3, 25°C, mutant enzyme W263I Saccharolobus solfataricus
3.1.1.25 121
-
 N-(3-oxododecanoyl)-L-homoserine lactone pH 8.3, 25°C, mutant enzyme W263V Saccharolobus solfataricus
3.1.1.25 259
-
 delta-undecanolactone pH 8.3, 25°C, mutant enzyme W263L Saccharolobus solfataricus
3.1.1.25 349
-
 gamma-undecanolactone pH 8.3, 25°C, mutant enzyme W263T Saccharolobus solfataricus
3.1.1.25 442
-
 delta-undecanolactone pH 8.3, 25°C, mutant enzyme W263M Saccharolobus solfataricus
3.1.1.25 492
-
 delta-undecanolactone pH 8.3, 25°C, mutant enzyme W263F Saccharolobus solfataricus
3.1.1.25 717
-
 delta-undecanolactone pH 8.3, 25°C, mutant enzyme W263T Saccharolobus solfataricus
3.1.1.25 792
-
 delta-undecanolactone pH 8.3, 25°C, mutant enzyme W263V Saccharolobus solfataricus