EC Number | Cloned (Comment) | Organism |
---|---|---|
1.8.4.12 | expressed in Escherichia coli BL21(DE3) cells and in yeast cytosol | Glycine max |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.8.4.12 | C121S | the mutant of isoform MSRB2 is inactive | Glycine max |
1.8.4.12 | C68S | the mutant of isoform MSRB2 is inactive | Glycine max |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.4.12 | 0.049 | - |
N-acetyl-L-methionine (R)-sulfoxide | isoform MSRB2, pH and temperature not specified in the publication | Glycine max | |
1.8.4.12 | 0.049 | - |
N-acetyl-L-methionine (R)-sulfoxide | isoform MSRB4, pH and temperature not specified in the publication | Glycine max | |
1.8.4.12 | 1.451 | - |
L-methionine (R)-sulfoxide | isoform MSRB4, pH and temperature not specified in the publication | Glycine max | |
1.8.4.12 | 2.093 | - |
L-methionine (R)-sulfoxide | isoform MSRB2, pH and temperature not specified in the publication | Glycine max |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.4.12 | L-methionine (R)-sulfoxide + thioredoxin | Glycine max | - |
L-methionine + thioredoxin disulfide + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.4.12 | Glycine max | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.8.4.12 | - |
Glycine max |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.4.12 | dabsyl-L-methionine (R)-sulfoxide + dithiothreitol | isoforms MSRB2 and MSRB4 also show enzyme activity toward protein-based L-methionine (R)-sulfoxide with either dithiothreitol or thioredoxin as reductants, whereas isoform MSRB1 is active only with dithiothreitol | Glycine max | dabsyl-L-methionine + dithiothreitol disulfide + H2O | - |
? | |
1.8.4.12 | L-methionine (R)-sulfoxide + dithiothreitol | - |
Glycine max | L-methionine + dithiothreitol disulfide + H2O | - |
? | |
1.8.4.12 | L-methionine (R)-sulfoxide + thioredoxin | - |
Glycine max | L-methionine + thioredoxin disulfide + H2O | - |
? | |
1.8.4.12 | N-acetyl-L-methionine (R)-sulfoxide + dithiothreitol | - |
Glycine max | N-acetyl-L-methionine + dithiothreitol disulfide + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.8.4.12 | methionine sulfoxide reductase B | - |
Glycine max |
1.8.4.12 | MsrB2 | isoform | Glycine max |
1.8.4.12 | MSRB4 | isoform | Glycine max |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.4.12 | 0.13 | - |
L-methionine (R)-sulfoxide | isoform MSRB4, pH and temperature not specified in the publication | Glycine max | |
1.8.4.12 | 0.24 | - |
N-acetyl-L-methionine (R)-sulfoxide | isoform MSRB4, pH and temperature not specified in the publication | Glycine max | |
1.8.4.12 | 2.04 | - |
N-acetyl-L-methionine (R)-sulfoxide | isoform MSRB2, pH and temperature not specified in the publication | Glycine max | |
1.8.4.12 | 2.86 | - |
L-methionine (R)-sulfoxide | isoform MSRB2, pH and temperature not specified in the publication | Glycine max |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.4.12 | 0.09 | - |
L-methionine (R)-sulfoxide | isoform MSRB4, pH and temperature not specified in the publication | Glycine max | |
1.8.4.12 | 1.4 | - |
L-methionine (R)-sulfoxide | isoform MSRB2, pH and temperature not specified in the publication | Glycine max | |
1.8.4.12 | 5 | - |
N-acetyl-L-methionine (R)-sulfoxide | isoform MSRB4, pH and temperature not specified in the publication | Glycine max | |
1.8.4.12 | 42 | - |
N-acetyl-L-methionine (R)-sulfoxide | isoform MSRB2, pH and temperature not specified in the publication | Glycine max |