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Literature summary extracted from

  • Rothe, M.; Alpert, C.; Loh, G.; Blaut, M.
    Novel insights into E. colis hexuronate metabolism: KduI facilitates the conversion of galacturonate and glucuronate under osmotic stress conditions (2013), PLoS ONE, 8, e56906.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.154 expresed as a His-tagged fusion protein in Escherichia coli Escherichia coli
5.3.1.17 expressed in Escherichia coli JM109 or KRX cells Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.154 crystal structure of AllD in its apo form is determined at 2.13 A resolution, as well as a binary complex at 1.64 A resolution with the NADH cofactor, and a ternary complex at 1.77 A resolution with NADH and glyoxylate, a product yielded from the spontaneous degradation of oxalurate Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.154 D141A Km ((S)-ureidoglycolate) increased, kcat (S-ureidoglycolate) or (NAD+) decreased compared to wild-type, Km (NAD+) similar to wo wild-type Escherichia coli
1.1.1.154 D141E Km ((S)-ureidoglycolate) increased, kcat ((S)-ureidoglycolate) or (NAD+) decreased compared to wild-type, Km (NAD+) decreased compared to wild-type Escherichia coli
1.1.1.154 D141N Km ((S)-ureidoglycolate) or (NAD+) increased, kcat ((S)-ureidoglycolate) or (NAD+) decreased compared to wild-type Escherichia coli
1.1.1.154 H116A mutant(S)-ureidoglycolate shows no activity Escherichia coli
1.1.1.154 H44A Km ((S)-ureidoglycolate) increased, kcat (S-ureidoglycolate) or (NAD+) decreased compared to wild-type, Km (NAD+) equal to wild-type Escherichia coli
1.1.1.154 M251A Km ((S)-ureidoglycolate) or (NAD+) increased, kcat ((S)-ureidoglycolate) or (NAD+) decreased compared to wild-type Escherichia coli
1.1.1.154 R259A Km ((S)-ureidoglycolate) or (NAD+) increased, kcat ((S)-ureidoglycolate) or (NAD+) decreased compared to wild-type Escherichia coli
1.1.1.154 R48A Km ((S)-ureidoglycolate) or (NAD+) increased, kcat ((S)-ureidoglycolate) or (NAD+) decreased compared to wild-type Escherichia coli
1.1.1.154 S140A Km ((S)-ureidoglycolate) or (NAD+) increased, kcat ((S)-ureidoglycolate) or (NAD+) decreased compared to wild-type Escherichia coli
1.1.1.154 S43A Km ((S)-ureidoglycolate) or (NAD+) increased, kcat ((S)-ureidoglycolate) or (NAD+) decreased compared to wild-type Escherichia coli
1.1.1.154 Y52F Km ((S)-ureidoglycolate) or (NAD+) increased, kcat ((S)-ureidoglycolate) or (NAD+) decreased compared to wild-type Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.154 0.37
-
 NAD+ mutant D141E, pH 8.1, 30°C Escherichia coli
1.1.1.154 0.52
-
 NAD+ mutant D141A, pH 8.1, 30°C Escherichia coli
1.1.1.154 0.56
-
 NAD+ wild-type, pH 8.1, 30°C Escherichia coli
1.1.1.154 0.65
-
 NAD+ mutant H44A, pH 8.1, 30°C Escherichia coli
1.1.1.154 0.81
-
 NAD+ mutant D141N, pH 8.1, 30°C Escherichia coli
1.1.1.154 0.93
-
 NAD+ mutant M251A, pH 8.1, 30°C Escherichia coli
1.1.1.154 1.06
-
 (S)-ureidoglycolate wild-type, pH 8.1, 30°C Escherichia coli
1.1.1.154 1.26
-
 NAD+ mutant Y52F, pH 8.1, 30°C Escherichia coli
1.1.1.154 1.39
-
 NAD+ mutant S140A, pH 8.1, 30°C Escherichia coli
1.1.1.154 1.49
-
 NAD+ mutant R259A, pH 8.1, 30°C Escherichia coli
1.1.1.154 2.28
-
 NAD+ mutant S43A, pH 8.1, 30°C Escherichia coli
1.1.1.154 5.27
-
 (S)-ureidoglycolate mutant D141A, pH 8.1, 30°C Escherichia coli
1.1.1.154 5.87
-
 (S)-ureidoglycolate mutant D141N, pH 8.1, 30°C Escherichia coli
1.1.1.154 10.75
-
 (S)-ureidoglycolate mutant S43A, pH 8.1, 30°C Escherichia coli
1.1.1.154 11.47
-
 (S)-ureidoglycolate mutant R259A, pH 8.1, 30°C Escherichia coli
1.1.1.154 12.38
-
 (S)-ureidoglycolate mutant S140A, pH 8.1, 30°C Escherichia coli
1.1.1.154 13.15
-
 (S)-ureidoglycolate mutant M251A, pH 8.1, 30°C Escherichia coli
1.1.1.154 14.13
-
 (S)-ureidoglycolate mutant D141E, pH 8.1, 30°C Escherichia coli
1.1.1.154 16.82
-
 (S)-ureidoglycolate mutant Y52F, pH 8.1, 30°C Escherichia coli
1.1.1.154 16.94
-
 (S)-ureidoglycolate mutant H44A, pH 8.1, 30°C Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.125 Escherichia coli
-
-
-
1.1.1.154 Escherichia coli
-
-
-
5.3.1.17 Escherichia coli
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.154 (S)-ureidoglycolate + NAD+
-
 Escherichia coli oxalureate + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.154 dimer gel filtration Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
1.1.1.125 2-deoxy-D-gluconate 3-dehydrogenase
-
 Escherichia coli
1.1.1.125 KduD
-
 Escherichia coli
1.1.1.154 (S)-ureidoglycolate dehydrogenase
-
 Escherichia coli
1.1.1.154 AllD
-
 Escherichia coli
5.3.1.17 5-keto 4-deoxyuronate isomerase
-
 Escherichia coli
5.3.1.17 KduI
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.154 30
-
 assay at Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.154 0.02
-
 NAD+ mutant S140A, pH 8.1, 30°C Escherichia coli
1.1.1.154 0.87
-
 NAD+ mutant D141E, pH 8.1, 30°C Escherichia coli
1.1.1.154 1.03
-
 NAD+ mutant H44A, pH 8.1, 30°C Escherichia coli
1.1.1.154 1.06
-
 NAD+ mutant D141A, pH 8.1, 30°C Escherichia coli
1.1.1.154 1.29
-
 (S)-ureidoglycolate mutant S140A, pH 8.1, 30°C Escherichia coli
1.1.1.154 1.37
-
 (S)-ureidoglycolate mutant S43A, pH 8.1, 30°C Escherichia coli
1.1.1.154 1.47
-
 NAD+ mutant D141N, pH 8.1, 30°C Escherichia coli
1.1.1.154 1.62
-
 (S)-ureidoglycolate mutant D141A, pH 8.1, 30°C Escherichia coli
1.1.1.154 1.67
-
 (S)-ureidoglycolate mutant D141E, pH 8.1, 30°C Escherichia coli
1.1.1.154 1.76
-
 (S)-ureidoglycolate mutant H44A, pH 8.1, 30°C Escherichia coli
1.1.1.154 1.9
-
 NAD+ mutant Y52F, pH 8.1, 30°C Escherichia coli
1.1.1.154 1.92
-
 (S)-ureidoglycolate mutant Y52F, pH 8.1, 30°C Escherichia coli
1.1.1.154 1.97
-
 (S)-ureidoglycolate mutant D141N, pH 8.1, 30°C Escherichia coli
1.1.1.154 2.51
-
 NAD+ mutant S43A, pH 8.1, 30°C Escherichia coli
1.1.1.154 9.29
-
 NAD+ mutant R259A, pH 8.1, 30°C Escherichia coli
1.1.1.154 11.98
-
 (S)-ureidoglycolate mutant R259A, pH 8.1, 30°C Escherichia coli
1.1.1.154 30.65
-
 NAD+ mutant M251A, pH 8.1, 30°C Escherichia coli
1.1.1.154 45.98
-
 (S)-ureidoglycolate mutant M251A, pH 8.1, 30°C Escherichia coli
1.1.1.154 57
-
 (S)-ureidoglycolate wild-type, pH 8.1, 30°C Escherichia coli
1.1.1.154 62
-
 NAD+ wild-type, pH 8.1, 30°C Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.154 8.1
-
 assay at Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.154 NADH
-
 Escherichia coli

Expression

EC Number Organism Comment Expression
1.1.1.125 Escherichia coli galacturonate and glucuronate induced kduD and kduI gene expression 3fold and 7 to 11fold, respectively, under aerobic conditions as well as 9 to 20fold and 19 to 54fold, respectively, under anaerobic conditions up
5.3.1.17 Escherichia coli 50 mM glucuronate induces kduI gene expression 7-11fold under aerobic conditions and 19-54fold under anaerobic conditions up

General Information

EC Number General Information Comment Organism
1.1.1.125 malfunction when grown in the presence of galacturonate or glucuronate, kduID deficient Escherichia coli have a 30% to 80% lower maximal cell density and 1.5 to 2fold longer doubling times under osmotic stress conditions than wild type Escherichia coli Escherichia coli
5.3.1.17 metabolism the KduI enzyme facilitates the conversion of galacturonate and glucuronate and facilitates the growth of Escherichia coli on galacturonate and glucuronate in the presence of osmotically active sucrose Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.154 0.01
-
 NAD+ mutant S140A, pH 8.1, 30°C Escherichia coli
1.1.1.154 0.1
-
 (S)-ureidoglycolate mutant H44A, pH 8.1, 30°C Escherichia coli
1.1.1.154 0.1
-
 (S)-ureidoglycolate mutant S140A, pH 8.1, 30°C Escherichia coli
1.1.1.154 0.11
-
 (S)-ureidoglycolate mutant Y52F, pH 8.1, 30°C Escherichia coli
1.1.1.154 0.12
-
 (S)-ureidoglycolate mutant D141E, pH 8.1, 30°C Escherichia coli
1.1.1.154 0.13
-
 (S)-ureidoglycolate mutant S43A, pH 8.1, 30°C Escherichia coli
1.1.1.154 0.31
-
 (S)-ureidoglycolate mutant D141A, pH 8.1, 30°C Escherichia coli
1.1.1.154 0.34
-
 (S)-ureidoglycolate mutant D141N, pH 8.1, 30°C Escherichia coli
1.1.1.154 1.04
-
 (S)-ureidoglycolate mutant R259A, pH 8.1, 30°C Escherichia coli
1.1.1.154 1.1
-
 NAD+ mutant S43A, pH 8.1, 30°C Escherichia coli
1.1.1.154 1.51
-
 NAD+ mutant Y52F, pH 8.1, 30°C Escherichia coli
1.1.1.154 1.6
-
 NAD+ mutant H44A, pH 8.1, 30°C Escherichia coli
1.1.1.154 1.81
-
 NAD+ mutant D141N, pH 8.1, 30°C Escherichia coli
1.1.1.154 2.04
-
 NAD+ mutant D141A, pH 8.1, 30°C Escherichia coli
1.1.1.154 2.35
-
 NAD+ mutant D141E, pH 8.1, 30°C Escherichia coli
1.1.1.154 3.49
-
 (S)-ureidoglycolate mutant M251A, pH 8.1, 30°C Escherichia coli
1.1.1.154 6.23
-
 NAD+ mutant R259A, pH 8.1, 30°C Escherichia coli
1.1.1.154 32.9
-
 NAD+ mutant M251A, pH 8.1, 30°C Escherichia coli
1.1.1.154 54
-
 (S)-ureidoglycolate wild-type, pH 8.1, 30°C Escherichia coli
1.1.1.154 110
-
 NAD+ wild-type, pH 8.1, 30°C Escherichia coli