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Literature summary extracted from

  • Zhang, J.; Kasciukovic, T.; White, M.F.
    The CRISPR associated protein Cas4 Is a 5' to 3' DNA exonuclease with an iron-sulfur cluster (2012), PLoS One, 7, e47232.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.12.1 expressed in Escherichia coli Posetta DE3 Saccharolobus solfataricus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.12.1 D99A the mutant enzyme fails to cut DNA Saccharolobus solfataricus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.12.1 iron-sulfur centre contains a [4Fe-4S] cluster Saccharolobus solfataricus
3.1.12.1 Mg2+ cleavage of a 15T DNA oligonucleotide by Sso0001 is observed in the presence of either magnesium or manganese, yielding a cluster of products of around 1–5 nt in size. A 20U RNA oligonucleotide is cleaved in the presence of manganese but not magnesium Saccharolobus solfataricus
3.1.12.1 Mn2+ cleavage of a 15T DNA oligonucleotide by Sso0001 is observed in the presence of either magnesium or manganese, yielding a cluster of products of around 1–5 nt in size. A 20U RNA oligonucleotide is cleaved in the presence of manganese but not magnesium Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.12.1 23239
-
 x * 23239, calculated from sequence Saccharolobus solfataricus
3.1.12.1 170000
-
 gel filtration Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
3.1.12.1 Saccharolobus solfataricus Q97TX9
-
-
3.1.12.1 Saccharolobus solfataricus P2 Q97TX9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.12.1
-
 Saccharolobus solfataricus

Storage Stability

EC Number Storage Stability Organism
3.1.12.1 -80°C, stable Saccharolobus solfataricus
3.1.12.1 4°C, enzyme loses colour and activity over several days Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.12.1 single-stranded oligodeoxyribonucleotide + H2O cleavage of a 15T DNA oligonucleotide is observed in the presence of either magnesium or manganese, yielding a cluster of products of around 1–5 nt in size. A 20U RNA oligonucleotide is cleaved in the presence of manganese but not magnesium. Saccharolobus solfataricus nucleoside 3'-phosphate + ?
-
 ?
3.1.12.1 single-stranded oligodeoxyribonucleotide + H2O cleavage of a 15T DNA oligonucleotide is observed in the presence of either magnesium or manganese, yielding a cluster of products of around 1–5 nt in size. A 20U RNA oligonucleotide is cleaved in the presence of manganese but not magnesium. Saccharolobus solfataricus P2 nucleoside 3'-phosphate + ?
-
 ?
3.1.12.1 ssDNA + H2O the enzyme is specific for ssDNA Saccharolobus solfataricus nucleoside 3'-phosphate + ?
-
 ?
3.1.12.1 ssDNA + H2O the enzyme is specific for ssDNA Saccharolobus solfataricus P2 nucleoside 3'-phosphate + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.12.1 ? x * 23239, calculated from sequence Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
3.1.12.1 5' to 3' single stranded DNA exonuclease
-
 Saccharolobus solfataricus
3.1.12.1 Cas4
-
 Saccharolobus solfataricus
3.1.12.1 SSO0001 locus name Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.12.1 6
-
 assay at Saccharolobus solfataricus

General Information

EC Number General Information Comment Organism
3.1.12.1 physiological function the role of the enzyme in vivo may be to process viral DNA to generate new spacers with 3' ssDNA overhangs suitable for recombination or integration in a CRISPR (Clusters of Regularly interspaced Palindromic Repeats) locus mediated by the enzyme Cas1 Saccharolobus solfataricus