EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.20.6 | additional information | silencing of FLS gene by RNAi, in lines G12, A2, B1 and E13, the quercetin content is increased by 351%, 49%, 157% and 258%, the silenced lines show arrest in pollen tube germination, and the pollen tubes grows only to about nine-tenths of the way down the style, and tube tips are swollen, contents of anthocyanins, epigallocatechin, and epicatechin are altered and content in indole acetic acid is reduced compared to the wild-type, overview | Nicotiana tabacum |
1.14.20.6 | additional information | silencing of FLS1 gene by RNAi, in lines G12, A2, B1 and E13, the quercetin content is increased by 351%, 49%, 157% and 258%, the silenced lines show arrest in pollen tube germination, and the pollen tubes grows only to about nine-tenths of the way down the style, and tube tips are swollen, contents of anthocyanins, epigallocatechin, and epicatechin are altered and content in indole acetic acid is reduced compared to the wild-type, overview | Nicotiana tabacum |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.20.6 | Fe2+ | required | Nicotiana tabacum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.20.6 | (2R/3R)-dihydroquercetin + 2-oxoglutarate + O2 | Nicotiana tabacum | - |
quercetin + succinate + CO2 + H2O | - |
? | |
1.14.20.6 | a dihydroflavonol + 2-oxoglutarate + O2 | Nicotiana tabacum | - |
a flavonol + succinate + CO2 + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.20.6 | Nicotiana tabacum | A9ZMJ0 | FLS; cv. Xanthi | - |
1.14.20.6 | Nicotiana tabacum | Q1PHN5 | FLS1; cv. Xanthi | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.20.6 | (2R/3R)-dihydroquercetin + 2-oxoglutarate + O2 | - |
Nicotiana tabacum | quercetin + succinate + CO2 + H2O | - |
? | |
1.14.20.6 | a dihydroflavonol + 2-oxoglutarate + O2 | - |
Nicotiana tabacum | a flavonol + succinate + CO2 + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.20.6 | FLS | - |
Nicotiana tabacum |
1.14.20.6 | FLS1 | - |
Nicotiana tabacum |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.20.6 | malfunction | post-transcriptional silencing of flavonol synthase mRNA in tobacco leads to fruits with arrested seed set, FLS silenced lines show 20-80% reduction in FLS encoding gene expression and 25-93% reduction in quercetin content, as well as a reduction in anthocyanidins content. Content of flavan-3-ols, i.e. catechin, epi-catechin and epigallocatechin, is increased in the transgenic lines, phenotypes, overview | Nicotiana tabacum |
1.14.20.6 | malfunction | post-transcriptional silencing of flavonol synthase mRNA in tobacco leads to fruits with arrested seed set, FLS1 silenced lines show 20-80% reduction in FLS1 encoding gene expression and 25-93% reduction in quercetin content, as well as a reduction in anthocyanidins content. Content of flavan-3-ols, i.e. catechin, epi-catechin and epigallocatechin, is increased in the transgenic lines, phenotypes, overview | Nicotiana tabacum |
1.14.20.6 | metabolism | the FLS enzyme competes with dihydroflavonol 4-reductase for the common substrate dihydroflavonols | Nicotiana tabacum |