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Literature summary extracted from

  • Berger, I.; Guttman, C.; Amar, D.; Zarivach, R.; Aharoni, A.
    The molecular basis for the broad substrate specificity of human sulfotransferase 1A1 (2011), PLoS ONE, 6, e26794.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.2.1 expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.8.2.1 in complex with adenosine 3',5'-bisphosphate alone, with adenosine 3',5'-bisphosphate and 3-cyano-7-hydroxycumarin and with adenosine 3',5'-bisphosphate and 2-naphthol, vapor diffusion method Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.8.2.1 D249G the mutant shows higher specific activity towards 3-cyano-7-hydroxycumarin (0.1 mM and above) compared to the wild type enzyme. The mutant is less heat stable than the wild type enzyme Homo sapiens
2.8.2.1 L67V/A101S/Q177K/V211L/F222K/F247I the mutant is more heat stable than the wild type enzyme Homo sapiens
2.8.2.1 Q56E/A101S/T117S/H213R/F222L/V243L/T266N/L111P/Y240C the mutant is more heat stable than the wild type enzyme Homo sapiens
2.8.2.1 Q56E/A101S/T117S/Q177K/M223I/V243L the mutant shows higher specific activity towards 3-cyano-7-hydroxycumarin (0.1 mM and above) compared to the wild type enzyme. The mutant is more heat stable than the wild type enzyme Homo sapiens
2.8.2.1 Q56E/L67V/A101S/T117S/H213R/F222L/V243I/D249G the mutant shows higher specific activity towards 3-cyano-7-hydroxycumarin (0.1 mM and above) compared to the wild type enzyme. The mutant is more heat stable than the wild type enzyme Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.1 0.02
-
 3-cyano-7-hydroxycoumarin wild type enzyme, in 50 mM HEPES, pH 7.5, 7 mM MgCl2, and 1.5 mM dithiothreitol, at 22°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.8.2.1 cytosol
-
 Homo sapiens 5829
-

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.1 Homo sapiens P50225
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.1 Ni-NTA resin column chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.1 3'-phosphoadenylyl sulfate + 2-naphthol
-
 Homo sapiens adenosine 3',5'-bisphosphate + 2-naphthyl sulfate
-
 ?
2.8.2.1 3'-phosphoadenylyl sulfate + 3-cyano-7-hydroxycoumarin
-
 Homo sapiens ?
-
 ?
2.8.2.1 3'-phosphoadenylyl sulfate + 4-nitrophenol
-
 Homo sapiens adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.8.2.1 sulfotransferase 1A1
-
 Homo sapiens
2.8.2.1 SULT1A1
-
 Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.8.2.1 30 60 the wild type enzyme remains stable up to 40°C and then rapidly loses activity being completely inactive at temperatures of 42°C and above Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.8.2.1 0.149
-
 3-cyano-7-hydroxycoumarin mutant enzyme Q56E/A101S/T117S/Q177K/M223I/V243L, in 50 mM HEPES, pH 7.5, 7 mM MgCl2, and 1.5 mM dithiothreitol, at 22°C Homo sapiens
2.8.2.1 0.308
-
 3-cyano-7-hydroxycoumarin mutant enzyme D249G, in 50 mM HEPES, pH 7.5, 7 mM MgCl2, and 1.5 mM dithiothreitol, at 22°C Homo sapiens
2.8.2.1 0.466
-
 3-cyano-7-hydroxycoumarin mutant enzyme Q56E/L67V/A101S/T117S/H213R/F222L/V243I/D249G, in 50 mM HEPES, pH 7.5, 7 mM MgCl2, and 1.5 mM dithiothreitol, at 22°C Homo sapiens
2.8.2.1 1.2
-
 3-cyano-7-hydroxycoumarin wild type enzyme, in 50 mM HEPES, pH 7.5, 7 mM MgCl2, and 1.5 mM dithiothreitol, at 22°C Homo sapiens