EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.3.1.9 | to 1.6 A resolution, space group P212121. The model consists of one monomer in the asymmetric unit which is composed of 13 alpha-helices and 11 beta-strands, representing a canonical Rossmann fold architecture. In addition to the conserved residues Y236 and K245 in the Y-X8-K motif, Y53, D111 and Y226 are key residues implicated in the reductase activity, and F113 and T276 are also important for enzyme function | Xanthomonas oryzae |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.3.1.9 | D111A | inactive | Xanthomonas oryzae |
1.3.1.9 | F113A | restores fatty acid synthesis in an Escherichia coli fabI mutant strain to wild-type level | Xanthomonas oryzae |
1.3.1.9 | K245A | inactive | Xanthomonas oryzae |
1.3.1.9 | K245R | inactive | Xanthomonas oryzae |
1.3.1.9 | S50A | restores fatty acid synthesis in an Escherichia coli fabI mutant strain to wild-type level | Xanthomonas oryzae |
1.3.1.9 | T276A | restores fatty acid synthesis in an Escherichia coli fabI mutant strain to wild-type level | Xanthomonas oryzae |
1.3.1.9 | V246A | restores fatty acid synthesis in an Escherichia coli fabI mutant strain to wild-type level | Xanthomonas oryzae |
1.3.1.9 | Y226F | restores fatty acid synthesis in an Escherichia coli fabI mutant strain to wild-type level | Xanthomonas oryzae |
1.3.1.9 | Y236A | inactive | Xanthomonas oryzae |
1.3.1.9 | Y236F | inactive | Xanthomonas oryzae |
1.3.1.9 | Y53A | partly restores fatty acid synthesis in an Escherichia coli fabI mutant strain | Xanthomonas oryzae |
1.3.1.9 | Y53F | restores fatty acid synthesis in an Escherichia coli fabI mutant strain to wild-type level | Xanthomonas oryzae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.1.9 | 0.0187 | - |
NADH | pH 7.5, 25°C | Xanthomonas oryzae | |
1.3.1.9 | 0.293 | - |
crotonyl-CoA | pH 7.5, 25°C | Xanthomonas oryzae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.1.9 | Xanthomonas oryzae | Q2P9J6 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.9 | crotonyl-CoA + NADH + H+ | - |
Xanthomonas oryzae | butyryl-CoA + NAD+ | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.1.9 | FabV | - |
Xanthomonas oryzae |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.1.9 | 22.25 | - |
crotonyl-CoA | pH 7.5, 25°C | Xanthomonas oryzae |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.9 | NADH | - |
Xanthomonas oryzae |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.1.9 | 75 | - |
crotonyl-CoA | pH 7.5, 25°C | Xanthomonas oryzae |