BRENDA - Enzyme Database

Catalytic properties of the isolated diaphorase fragment of the NAD +-reducing [NiFe]-hydrogenase from Ralstonia eutropha

Lauterbach, L.; Idris, Z.; Vincent, K.; Lenz, O.; PLoS ONE 6, e25939 (2011)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.12.1.2
NAD+
product inhibition; product inhibition
Cupriavidus necator
1.12.1.2
NADH
product inhibition; product inhibition
Cupriavidus necator
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.12.1.2
0.056
-
NADH
pH 8.0, temperature not specified in the publication; pH 8.0, temperature not specified in the publication
Cupriavidus necator
1.12.1.2
0.197
-
NAD+
pH 8.0, temperature not specified in the publication; pH 8.0, temperature not specified in the publication
Cupriavidus necator
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.12.1.2
Cupriavidus necator
P22317
subunit alpha
-
1.12.1.2
Cupriavidus necator
P22318
subunit gamma
-
1.12.1.2
Cupriavidus necator DSM 428
P22317
subunit alpha
-
1.12.1.2
Cupriavidus necator DSM 428
P22318
subunit gamma
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.12.1.2
H+ + NADH
module HoxFU shows clear electrocatalytic activity for both NAD+ reduction and NADH oxidation with minimal overpotential relative to the potential of the NAD+/NADH couple
726282
Cupriavidus necator
H2 + NAD+
-
-
-
r
1.12.1.2
H+ + NADH
module HoxFU shows clear electrocatalytic activity for both NAD+ reduction and NADH oxidation with minimal overpotential relative to the potential of the NAD+/NADH couple
726282
Cupriavidus necator DSM 428
H2 + NAD+
-
-
-
r
1.12.1.2
H2 + NAD+
module HoxFU shows clear electrocatalytic activity for both NAD+ reduction and NADH oxidation with minimal overpotential relative to the potential of the NAD+/NADH couple
726282
Cupriavidus necator
H+ + NADH
-
-
-
r
1.12.1.2
H2 + NAD+
module HoxFU shows clear electrocatalytic activity for both NAD+ reduction and NADH oxidation with minimal overpotential relative to the potential of the NAD+/NADH couple
726282
Cupriavidus necator DSM 428
H+ + NADH
-
-
-
r
1.12.1.2
additional information
in protein film electrochemical experiments the electrocatalytic current is unaffected by O2. In aerobic solution assays, a moderate superoxide production rate of 54 nmol per mg of protein is observed, meaning that the formation of reactive oxygen species observed for the native enzyme can be attributed mainly to module HoxFU
726282
Cupriavidus necator
?
-
-
-
-
1.12.1.2
additional information
in protein film electrochemical experiments the electrocatalytic current is unaffected by O2. In aerobic solution assays, a moderate superoxide production rate of 54 nmol per mg of protein is observed, meaning that the formation of reactive oxygen species observed for the native enzyme can be attributed mainly to module HoxFU
726282
Cupriavidus necator DSM 428
?
-
-
-
-
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.12.1.2
FMN
module HoxFU accommodates a [2Fe2S] cluster, FMN and a series of [4Fe4S] clusters; module HoxFU accommodates a [2Fe2S] cluster, FMN and a series of [4Fe4S] clusters
Cupriavidus necator
1.12.1.2
[2Fe-2S]-center
HoxFU accommodates a [2Fe2S] cluster, FMN and a series of [4Fe4S] clusters; HoxFU accommodates a [2Fe2S] cluster, FMN and a series of [4Fe4S] clusters
Cupriavidus necator
1.12.1.2
[4Fe-4S]-center
HoxFU accommodates a [2Fe2S] cluster, FMN and a series of [4Fe4S] clusters; HoxFU accommodates a [2Fe2S] cluster, FMN and a series of [4Fe4S] clusters
Cupriavidus necator
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.12.1.2
0.2
-
NAD+
pH 8.0, temperature not specified in the publication; pH 8.0, temperature not specified in the publication
Cupriavidus necator
1.12.1.2
0.2
-
NADH
pH 8.0, temperature not specified in the publication; pH 8.0, temperature not specified in the publication
Cupriavidus necator
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.12.1.2
FMN
module HoxFU accommodates a [2Fe2S] cluster, FMN and a series of [4Fe4S] clusters
Cupriavidus necator
1.12.1.2
[2Fe-2S]-center
HoxFU accommodates a [2Fe2S] cluster, FMN and a series of [4Fe4S] clusters
Cupriavidus necator
1.12.1.2
[4Fe-4S]-center
HoxFU accommodates a [2Fe2S] cluster, FMN and a series of [4Fe4S] clusters
Cupriavidus necator
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.12.1.2
NAD+
product inhibition
Cupriavidus necator
1.12.1.2
NADH
product inhibition
Cupriavidus necator
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.12.1.2
0.2
-
NAD+
pH 8.0, temperature not specified in the publication
Cupriavidus necator
1.12.1.2
0.2
-
NADH
pH 8.0, temperature not specified in the publication
Cupriavidus necator
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.12.1.2
0.056
-
NADH
pH 8.0, temperature not specified in the publication
Cupriavidus necator
1.12.1.2
0.197
-
NAD+
pH 8.0, temperature not specified in the publication
Cupriavidus necator
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.12.1.2
H+ + NADH
module HoxFU shows clear electrocatalytic activity for both NAD+ reduction and NADH oxidation with minimal overpotential relative to the potential of the NAD+/NADH couple
726282
Cupriavidus necator
H2 + NAD+
-
-
-
r
1.12.1.2
H+ + NADH
module HoxFU shows clear electrocatalytic activity for both NAD+ reduction and NADH oxidation with minimal overpotential relative to the potential of the NAD+/NADH couple
726282
Cupriavidus necator DSM 428
H2 + NAD+
-
-
-
r
1.12.1.2
H2 + NAD+
module HoxFU shows clear electrocatalytic activity for both NAD+ reduction and NADH oxidation with minimal overpotential relative to the potential of the NAD+/NADH couple
726282
Cupriavidus necator
H+ + NADH
-
-
-
r
1.12.1.2
H2 + NAD+
module HoxFU shows clear electrocatalytic activity for both NAD+ reduction and NADH oxidation with minimal overpotential relative to the potential of the NAD+/NADH couple
726282
Cupriavidus necator DSM 428
H+ + NADH
-
-
-
r
1.12.1.2
additional information
in protein film electrochemical experiments the electrocatalytic current is unaffected by O2. In aerobic solution assays, a moderate superoxide production rate of 54 nmol per mg of protein is observed, meaning that the formation of reactive oxygen species observed for the native enzyme can be attributed mainly to module HoxFU
726282
Cupriavidus necator
?
-
-
-
-
1.12.1.2
additional information
in protein film electrochemical experiments the electrocatalytic current is unaffected by O2. In aerobic solution assays, a moderate superoxide production rate of 54 nmol per mg of protein is observed, meaning that the formation of reactive oxygen species observed for the native enzyme can be attributed mainly to module HoxFU
726282
Cupriavidus necator DSM 428
?
-
-
-
-