BRENDA - Enzyme Database

Tartronate semialdehyde reductase defines a novel rate-limiting step in assimilation and bioconversion of glycerol in Ustilago maydis

Liu, Y.; Koh, C.; Sun, L.; Ji, L.; PLoS ONE 6, e16438 (2011)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.1.1.60
expression in Escherichia coli
Ustilago maydis
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.60
EDTA
severe inhibition
Ustilago maydis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.60
0.00019
-
2-hydroxy-3-oxopropanoate
pH 8.5, 40°C
Ustilago maydis
1.1.1.60
0.0003
-
NADP+
pH 8.5, 40°C
Ustilago maydis
1.1.1.60
0.0143
-
NADH
pH 8.5, 40°C
Ustilago maydis
1.1.1.60
0.0177
-
D-glycerate
pH 8.5, 40°C
Ustilago maydis
1.1.1.60
0.123
-
L-Glycerate
pH 8.5, 40°C
Ustilago maydis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.60
40000
-
2 * 40000, SDS-PAGE, 2 * 41000, calculated, recombinant protein
Ustilago maydis
1.1.1.60
41000
-
2 * 40000, SDS-PAGE, 2 * 41000, calculated, recombinant protein
Ustilago maydis
1.1.1.60
85000
-
gel filtration
Ustilago maydis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.60
Ustilago maydis
Q4PBC1
putative
-
Storage Stability
EC Number
Storage Stability
Organism
1.1.1.60
4°C, phosphate buffer, pH 7.0, improved solubility
Ustilago maydis
1.1.1.60
4°C, Tris-HCl, pH 8.0, highly unstable, precipitates during storage
Ustilago maydis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.60
2-hydroxy-3-oxopropanoate + NADH + H+
-
726270
Ustilago maydis
D-glycerate + NAD+
-
-
-
?
1.1.1.60
D-glycerate + NADP+
enzyme shows strong specificity and enantioselectivity for D-glycerate
726270
Ustilago maydis
2-hydroxy-3-oxopropanoate + NADPH + H+
-
-
-
?
1.1.1.60
L-glycerate + NADP+
enzyme shows strong specificity and enantioselectivity for D-glycerate
726270
Ustilago maydis
2-hydroxy-3-oxopropanoate + NADPH + H+
-
-
-
?
1.1.1.60
additional information
poor activity towards 6-phosphogluconic acid, beta-hydroxybutyric acid and D-threonine
726270
Ustilago maydis
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.60
dimer
2 * 40000, SDS-PAGE, 2 * 41000, calculated, recombinant protein
Ustilago maydis
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.60
40
-
-
Ustilago maydis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.60
8.5
-
-
Ustilago maydis
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.1.1.60
6.5
-
rapidly precipitates below
Ustilago maydis
1.1.1.60
7
-
phosphate buffer, pH 7.0, improved solubility
Ustilago maydis
1.1.1.60
8
-
Tris-HCl, highly unstable, precipitates during storage
Ustilago maydis
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.60
NAD+
about 75% of the activity with NADP+
Ustilago maydis
1.1.1.60
NADP+
preferred over NAD+
Ustilago maydis
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.60
expression in Escherichia coli
Ustilago maydis
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.60
NAD+
about 75% of the activity with NADP+
Ustilago maydis
1.1.1.60
NADP+
preferred over NAD+
Ustilago maydis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.60
EDTA
severe inhibition
Ustilago maydis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.60
0.00019
-
2-hydroxy-3-oxopropanoate
pH 8.5, 40°C
Ustilago maydis
1.1.1.60
0.0003
-
NADP+
pH 8.5, 40°C
Ustilago maydis
1.1.1.60
0.0143
-
NADH
pH 8.5, 40°C
Ustilago maydis
1.1.1.60
0.0177
-
D-glycerate
pH 8.5, 40°C
Ustilago maydis
1.1.1.60
0.123
-
L-Glycerate
pH 8.5, 40°C
Ustilago maydis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.60
40000
-
2 * 40000, SDS-PAGE, 2 * 41000, calculated, recombinant protein
Ustilago maydis
1.1.1.60
41000
-
2 * 40000, SDS-PAGE, 2 * 41000, calculated, recombinant protein
Ustilago maydis
1.1.1.60
85000
-
gel filtration
Ustilago maydis
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.1.1.60
4°C, phosphate buffer, pH 7.0, improved solubility
Ustilago maydis
1.1.1.60
4°C, Tris-HCl, pH 8.0, highly unstable, precipitates during storage
Ustilago maydis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.60
2-hydroxy-3-oxopropanoate + NADH + H+
-
726270
Ustilago maydis
D-glycerate + NAD+
-
-
-
?
1.1.1.60
D-glycerate + NADP+
enzyme shows strong specificity and enantioselectivity for D-glycerate
726270
Ustilago maydis
2-hydroxy-3-oxopropanoate + NADPH + H+
-
-
-
?
1.1.1.60
L-glycerate + NADP+
enzyme shows strong specificity and enantioselectivity for D-glycerate
726270
Ustilago maydis
2-hydroxy-3-oxopropanoate + NADPH + H+
-
-
-
?
1.1.1.60
additional information
poor activity towards 6-phosphogluconic acid, beta-hydroxybutyric acid and D-threonine
726270
Ustilago maydis
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.60
dimer
2 * 40000, SDS-PAGE, 2 * 41000, calculated, recombinant protein
Ustilago maydis
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.60
40
-
-
Ustilago maydis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.60
8.5
-
-
Ustilago maydis
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.1.1.60
6.5
-
rapidly precipitates below
Ustilago maydis
1.1.1.60
7
-
phosphate buffer, pH 7.0, improved solubility
Ustilago maydis
1.1.1.60
8
-
Tris-HCl, highly unstable, precipitates during storage
Ustilago maydis
General Information
EC Number
General Information
Commentary
Organism
1.1.1.60
physiological function
over-expression of the gene results in improved glycerol assimilation. Glycolipid accumulation is reduced by 45.2% in the knockout mutant whereas over-expression of the gene increases it by 40.4%
Ustilago maydis
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.1.1.60
physiological function
over-expression of the gene results in improved glycerol assimilation. Glycolipid accumulation is reduced by 45.2% in the knockout mutant whereas over-expression of the gene increases it by 40.4%
Ustilago maydis