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Literature summary extracted from

  • Moccand, C.; Kaufmann, M.; Fitzpatrick, T.
    It takes two to Tango: Defining an essential second active site in pyridoxal 5'-phosphate synthase (2011), PLoS ONE, 6, e16042.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.3.6 Pdx1 and Pdx2 with and without His-tag expressed Bacillus subtilis

Protein Variants

EC Number Protein Variants Comment Organism
4.3.3.6 E105D Pdx1 Bacillus subtilis
4.3.3.6 H115A Pdx1 Bacillus subtilis
4.3.3.6 H115A/R138A Pdx1 Bacillus subtilis
4.3.3.6 H170N Pdx2, catalytically inert Bacillus subtilis
4.3.3.6 K187A Pdx1, 84% of wild-type activity Bacillus subtilis
4.3.3.6 R138A Pdx1 Bacillus subtilis
4.3.3.6 R288A Pdx1, able to activate Pdx2 Bacillus subtilis
4.3.3.6 R288K Pdx1, able to activate Pdx2 Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.3.6 0.026
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, His-tagged K187A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.032
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, wild-type protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.035
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, His-tagged H115A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.04
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, His-tagged E105D mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.04
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, His-tagged wild-type protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.043
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, His-tagged H115A/R138A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.043
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, R288K mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.044
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, His-tagged R138A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.122
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, R288A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.155
-
 glyceraldehyde 3-phosphate pyridoxal 5'-phosphate formation, His-tagged wild-type protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.186
-
 glyceraldehyde 3-phosphate pyridoxal 5'-phosphate formation, wild-type protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.195
-
 glyceraldehyde 3-phosphate pyridoxal 5'-phosphate formation, R288K mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.22
-
 glyceraldehyde 3-phosphate pyridoxal 5'-phosphate formation, R288A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.229
-
 glyceraldehyde 3-phosphate pyridoxal 5'-phosphate formation, His-tagged H115A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.249
-
 glyceraldehyde 3-phosphate pyridoxal 5'-phosphate formation, His-tagged E105D mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.262
-
 glyceraldehyde 3-phosphate pyridoxal 5'-phosphate formation, His-tagged R138A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.267
-
 glyceraldehyde 3-phosphate pyridoxal 5'-phosphate formation, His-tagged K187A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.307
-
 glyceraldehyde 3-phosphate pyridoxal 5'-phosphate formation, His-tagged H115A/R138A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.93
-
 L-glutamine Pdx2 glutaminase activity, R288K mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 1.12
-
 L-glutamine Pdx2 glutaminase activity, His-tagged E105D mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 1.16
-
 L-glutamine Pdx2 glutaminase activity, His-tagged wild-type protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 1.16
-
 L-glutamine Pdx2 glutaminase activity, wild-type protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 1.2
-
 L-glutamine Pdx2 glutaminase activity, R288A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 1.31
-
 L-glutamine Pdx2 glutaminase activity, His-tagged K187A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 1.33
-
 L-glutamine Pdx2 glutaminase activity, His-tagged H115A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 1.44
-
 L-glutamine Pdx2 glutaminase activity, His-tagged H115A/R138A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 1.54
-
 L-glutamine Pdx2 glutaminase activity, His-tagged R138A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.3.6 32830
-
 unmodified Pdx1, ESI-MS Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
4.3.3.6 Bacillus subtilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.3.6
-
 Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.3.6 D-ribose 5-phosphate + glyceraldehyde 3-phosphate + L-glutamine in the presence of Pdx1 and Pdx2 Bacillus subtilis pyridoxal 5'-phosphate + L-glutamate + 3 H2O + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.3.3.6 pyridoxal 5'-phosphate synthase
-
 Bacillus subtilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.3.3.6 0.0001
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, His-tagged H115A/R138A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.00013
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, His-tagged H115A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.0002
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, His-tagged E105D mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.0002
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, His-tagged R138A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.0005
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, His-tagged K187A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.0005
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, R288A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.00055
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, wild-type protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.0006
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, His-tagged wild-type protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.00062
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, R288K mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.022
-
 L-glutamine Pdx2 glutaminase activity, His-tagged H115A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.024
-
 L-glutamine Pdx2 glutaminase activity, His-tagged H115A/R138A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.024
-
 L-glutamine Pdx2 glutaminase activity, His-tagged K187A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.024
-
 L-glutamine Pdx2 glutaminase activity, His-tagged R138A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.024
-
 L-glutamine Pdx2 glutaminase activity, His-tagged wild-type protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.026
-
 L-glutamine Pdx2 glutaminase activity, His-tagged E105D mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.0295
-
 L-glutamine Pdx2 glutaminase activity, wild-type protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.0305
-
 L-glutamine Pdx2 glutaminase activity, R288A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.0307
-
 L-glutamine Pdx2 glutaminase activity, R288K mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.3.3.6 0.00033
-
 glyceraldehyde 3-phosphate pyridoxal 5'-phosphate formation, His-tagged H115A/R138A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.00058
-
 glyceraldehyde 3-phosphate pyridoxal 5'-phosphate formation, His-tagged H115A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.00077
-
 glyceraldehyde 3-phosphate pyridoxal 5'-phosphate formation, His-tagged R138A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.00093
-
 glyceraldehyde 3-phosphate pyridoxal 5'-phosphate formation, His-tagged E105D mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.0019
-
 glyceraldehyde 3-phosphate pyridoxal 5'-phosphate formation, His-tagged K187A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.0023
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, His-tagged H115A/R138A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.0023
-
 glyceraldehyde 3-phosphate pyridoxal 5'-phosphate formation, R288A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.003
-
 glyceraldehyde 3-phosphate pyridoxal 5'-phosphate formation, wild-type protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.0032
-
 glyceraldehyde 3-phosphate pyridoxal 5'-phosphate formation, R288K mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.0038
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, His-tagged H115A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.0039
-
 glyceraldehyde 3-phosphate pyridoxal 5'-phosphate formation, His-tagged wild-type protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.0041
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, R288A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.0046
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, His-tagged R138A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.0058
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, His-tagged E105D mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.015
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, His-tagged wild-type protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.0154
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, R288K mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.016
-
 L-glutamine Pdx2 glutaminase activity, His-tagged H115A/R138A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.016
-
 L-glutamine Pdx2 glutaminase activity, His-tagged R138A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.017
-
 L-glutamine Pdx2 glutaminase activity, His-tagged H115A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.0172
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, wild-type protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.018
-
 L-glutamine Pdx2 glutaminase activity, His-tagged K187A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.0192
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, His-tagged K187A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.021
-
 L-glutamine Pdx2 glutaminase activity, His-tagged wild-type protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.024
-
 L-glutamine Pdx2 glutaminase activity, His-tagged E105D mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.026
-
 L-glutamine Pdx2 glutaminase activity, R288A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.026
-
 L-glutamine Pdx2 glutaminase activity, wild-type protein, pH 7.5, 37°C Bacillus subtilis
4.3.3.6 0.033
-
 L-glutamine Pdx2 glutaminase activity, R288K mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis