EC Number | Cloned (Comment) | Organism |
---|---|---|
1.4.1.4 | gene gdhA, phylogenetic analysis, expression of His6-tagged GdhA in Escherichia coli, ectopic expression in the cytoplasm of transgenic Oryza sativa plants. The introduced gdhA, together with the endogenous glutamine synthetase, directly assimilates NH4 + absorbed from the roots. NADP(H)-GDH activities in the gdhA-transgenic lines are markedly higher than those in a control line | Aspergillus niger |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.1.4 | 0.041 | - |
NADP+ | pH 8.0, 30°C, recombinant enzyme | Aspergillus niger | |
1.4.1.4 | 0.34 | - |
NADPH | pH 8.0, 30°C, recombinant enzyme | Aspergillus niger | |
1.4.1.4 | 1.16 | - |
NH3 | pH 8.0, 30°C, recombinant enzyme | Aspergillus niger | |
1.4.1.4 | 3.97 | - |
2-oxoglutarate | pH 8.0, 30°C, recombinant enzyme | Aspergillus niger | |
1.4.1.4 | 15.15 | - |
L-glutamate | pH 8.0, 30°C, recombinant enzyme | Aspergillus niger |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.4 | additional information | Ca2+ has no effects on the aminating activity of NADP(H)-GDH | Aspergillus niger |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.1.4 | L-glutamate + H2O + NADP+ | Aspergillus niger | - |
2-oxoglutarate + NH3 + NADPH + H+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.1.4 | Aspergillus niger | - |
gene gdhA | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.4.1.4 | recombinant His6-tagged GdhA from Escherichia coli by nickel affinity chromatography | Aspergillus niger |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.4.1.4 | root | - |
Aspergillus niger | - |
1.4.1.4 | shoot | - |
Aspergillus niger | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.1.4 | L-glutamate + H2O + NADP+ | - |
Aspergillus niger | 2-oxoglutarate + NH3 + NADPH + H+ | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.4.1.4 | GDH | - |
Aspergillus niger |
1.4.1.4 | NADP(H)-dependent glutamate dehydrogenase | - |
Aspergillus niger |
1.4.1.4 | NADP(H)-GDH | - |
Aspergillus niger |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.4.1.4 | 30 | - |
assay at | Aspergillus niger |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.4.1.4 | 8 | - |
assay at | Aspergillus niger |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.4 | NADP+ | - |
Aspergillus niger | |
1.4.1.4 | NADPH | - |
Aspergillus niger |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.4.1.4 | metabolism | both NADP(H)-GDH (gdhA) and glutamine synthetase play important roles in ammonium assimilation | Aspergillus niger |