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Literature summary extracted from

  • Gisby, M.F.; Mudd, E.A.; Day, A.
    Growth of transplastomic cells expressing D-amino acid oxidase in chloroplasts is tolerant to D-alanine and inhibited by D-valine (2012), Plant Physiol., 160, 2219-2226.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.3 expressed in Escherichia coli strain BL21(DE3)pLysS and in Nicotiana tabacum plastids Schizosaccharomyces pombe

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.3 35000
-
 x * 35000, SDS-PAGE Schizosaccharomyces pombe
1.4.3.3 39000
-
 x * 39000, calculated from amino acid sequence Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.3 D-amino acid + H2O + O2 Schizosaccharomyces pombe
-
 2-oxocarboxylate + NH3 + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.3 Schizosaccharomyces pombe
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.3 D-alanine + H2O + O2
-
 Schizosaccharomyces pombe pyruvate + NH3 + H2O2
-
 ?
1.4.3.3 D-amino acid + H2O + O2
-
 Schizosaccharomyces pombe 2-oxocarboxylate + NH3 + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.3 ? x * 35000, SDS-PAGE Schizosaccharomyces pombe
1.4.3.3 ? x * 39000, calculated from amino acid sequence Schizosaccharomyces pombe

Synonyms

EC Number Synonyms Comment Organism
1.4.3.3 D-amino acid oxidase
-
 Schizosaccharomyces pombe
1.4.3.3 DAAO
-
 Schizosaccharomyces pombe

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.3 FAD
-
 Schizosaccharomyces pombe

General Information

EC Number General Information Comment Organism
1.4.3.3 physiological function expression of a D-amino acid oxidase gene from Schizosaccharomyces pombe in tobacco chloroplasts give rise to transplastomic ptdao plants that resemble wild-type plants in growth and appearance. Growth of ptdao cells is tolerant to D-alanine but inhibited by D-valine Schizosaccharomyces pombe