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Literature summary extracted from

  • Tieman, D.; Zeigler, M.; Schmelz, E.; Taylor, M.G.; Rushing, S.; Jones, J.B.; Klee, H.J.
    Functional analysis of a tomato salicylic acid methyl transferase and its role in synthesis of the flavor volatile methyl salicylate (2010), Plant J., 62, 113-123.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.274 expressed in Escherichia coli BL21-AI cells Solanum lycopersicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.274 0.0146
-
S-adenosyl-L-methionine in 50 mM Tris-HCl, pH 7.5, 100 mM KCl, at 25°C Solanum lycopersicum
2.1.1.274 0.05
-
salicylate in 50 mM Tris-HCl, pH 7.5, 100 mM KCl, at 25°C Solanum lycopersicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.274 additional information Solanum lycopersicum less than 1% activity with jasmonic acid, indole-3-acetic acid and gibberellic acid 3 ?
-
?
2.1.1.274 S-adenosyl-L-methionine + 4-aminosalicylate Solanum lycopersicum 2% activity compared to salicylate S-adenosyl-L-homocysteine + methyl 4-aminosalicylate
-
?
2.1.1.274 S-adenosyl-L-methionine + benzoate Solanum lycopersicum 2% activity compared to salicylate S-adenosyl-L-homocysteine + methyl benzoate
-
?
2.1.1.274 S-adenosyl-L-methionine + salicylate Solanum lycopersicum 100% activity S-adenosyl-L-homocysteine + methyl salicylate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.274 Solanum lycopersicum
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.274 bud
-
Solanum lycopersicum
-
2.1.1.274 flower
-
Solanum lycopersicum
-
2.1.1.274 fruit
-
Solanum lycopersicum
-
2.1.1.274 leaf
-
Solanum lycopersicum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.274 additional information less than 1% activity with jasmonic acid, indole-3-acetic acid and gibberellic acid 3 Solanum lycopersicum ?
-
?
2.1.1.274 S-adenosyl-L-methionine + 4-aminosalicylate 2% activity compared to salicylate Solanum lycopersicum S-adenosyl-L-homocysteine + methyl 4-aminosalicylate
-
?
2.1.1.274 S-adenosyl-L-methionine + benzoate 2% activity compared to salicylate Solanum lycopersicum S-adenosyl-L-homocysteine + methyl benzoate
-
?
2.1.1.274 S-adenosyl-L-methionine + salicylate 100% activity Solanum lycopersicum S-adenosyl-L-homocysteine + methyl salicylate
-
?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.274 salicylic acid methyl transferase
-
Solanum lycopersicum
2.1.1.274 SAMT
-
Solanum lycopersicum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.274 0.028
-
S-adenosyl-L-methionine in 50 mM Tris-HCl, pH 7.5, 100 mM KCl, at 25°C Solanum lycopersicum
2.1.1.274 0.056
-
salicylate in 50 mM Tris-HCl, pH 7.5, 100 mM KCl, at 25°C Solanum lycopersicum

Expression

EC Number Organism Comment Expression
2.1.1.274 Solanum lycopersicum isoform SAMT1 expression drops off to essentially undetectable levels by the time fruits are fully ripened down

General Information

EC Number General Information Comment Organism
2.1.1.274 metabolism the enzyme is critical for methyl salicylate synthesis Solanum lycopersicum