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Literature summary extracted from

  • Asada, K.; Salim, V.; Masada-Atsumi, S.; Edmunds, E.; Nagatoshi, M.; Terasaka, K.; Mizukami, H.; de Luca, V.
    A 7-deoxyloganetic acid glucosyltransferase contributes a key step in secologanin biosynthesis in madagascar periwinkle (2013), Plant Cell, 25, 4123-4134.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.323 expression in Escherichia coli Catharanthus roseus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.323 0.088
-
 UDP-alpha-D-glucose pH 8.0, 30°C Catharanthus roseus
2.4.1.323 5.38
-
 7-deoxyloganetate pH 8.0, 30°C Catharanthus roseus
2.4.1.324 0.117
-
 UDP-alpha-D-glucose pH 8.0, 30°C Catharanthus roseus
2.4.1.324 0.202
-
 7-deoxyloganetin pH 8.0, 30°C Catharanthus roseus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.323 UDP-alpha-D-glucose + 7-deoxyloganetate Catharanthus roseus the enzyme is involved in loganin and secologanin biosynthesis UDP + 7-deoxyloganate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.323 Catharanthus roseus U3U992
-
-
2.4.1.324 Catharanthus roseus F8WLS6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.323 recombinant enzyme, purified by nickel nitrilotriacetic acid affinity chromatography Catharanthus roseus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.323 leaf preferentially expressed in internal phloem associated parenchyma cells Catharanthus roseus
-
2.4.1.323 phloem preferentially expressed in internal phloem associated parenchyma cells Catharanthus roseus
-
2.4.1.323 plant parenchyma preferentially expressed in internal phloem associated parenchyma cells Catharanthus roseus
-
2.4.1.324 leaf
-
 Catharanthus roseus
-
2.4.1.324 root preferential expression Catharanthus roseus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.323 UDP-alpha-D-glucose + 7-deoxyloganetate the enzyme is involved in loganin and secologanin biosynthesis Catharanthus roseus UDP + 7-deoxyloganate
-
 ?
2.4.1.323 UDP-alpha-D-glucose + 7-deoxyloganetate does not react with 7-deoxyloganetin Catharanthus roseus UDP + 7-deoxyloganate
-
 ?
2.4.1.324 UDP-alpha-D-glucose + 7-deoxyloganetin strict substrate specificity toward 7-deoxyloganetin Catharanthus roseus UDP + 7-deoxyloganin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.323 UGT8
-
 Catharanthus roseus
2.4.1.324 UGT6
-
 Catharanthus roseus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.323 30
-
 assay at Catharanthus roseus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.323 0.13
-
 UDP-alpha-D-glucose pH 8.0, 30°C Catharanthus roseus
2.4.1.323 0.325
-
 7-deoxyloganetate pH 8.0, 30°C Catharanthus roseus
2.4.1.324 0.032
-
 UDP-alpha-D-glucose pH 8.0, 30°C Catharanthus roseus
2.4.1.324 0.0355
-
 7-deoxyloganetin pH 8.0, 30°C Catharanthus roseus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.323 8
-
 assay at Catharanthus roseus

General Information

EC Number General Information Comment Organism
2.4.1.323 physiological function the enzyme is involved in loganin and secologanin biosynthesis Catharanthus roseus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.323 0.064
-
 7-deoxyloganetate pH 8.0, 30°C Catharanthus roseus
2.4.1.323 1.5
-
 UDP-alpha-D-glucose pH 8.0, 30°C Catharanthus roseus
2.4.1.324 0.179
-
 7-deoxyloganetin pH 8.0, 30°C Catharanthus roseus
2.4.1.324 0.291
-
 UDP-alpha-D-glucose pH 8.0, 30°C Catharanthus roseus