Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Dudareva, N.; Murfitt, L.M.; Mann, C.J.; Gorenstein, N.; Kolosova, N.; Kish, C.M.; Bonham, C.; Wood, K.
    Developmental regulation of methyl benzoate biosynthesis and emission in snapdragon flowers (2000), Plant Cell, 12, 949-961.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.273 BAMT cDNA expressed in Escherichia coli Clarkia breweri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.273 49000
-
 SDS-PAGE Clarkia breweri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.273 S-adenosyl-L-methionine + benzoate Clarkia breweri emission of methyl benzoate occurs in a rhythmic manner, with maximum emission during the day, correlating with pollinator activity S-adenosyl-L-homocysteine + methyl benzoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.273 Clarkia breweri
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.273 from 5- to 10-day-old upper and lower petal lobes by DE53 anion exchange, phenyl–Sepharose 6FF and MonoQ chromatography Clarkia breweri

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.273 anther reduced BAMT activity Clarkia breweri
-
2.1.1.273 corolla tube reduced BAMT activity Clarkia breweri
-
2.1.1.273 additional information no BAMT activity in pistil, stamen, sepal, ovary and leaf tissue Clarkia breweri
-
2.1.1.273 petal in upper and lower lobes, major activity of BAMT Clarkia breweri
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.273 additional information
-
 emission of methyl benzoate begins at anthesis but at a very low level, 1.8 mg per flower for 24 hr, reaches a peak between days 5 to 8, and declines thereafter, at peak, 56.5 mg is emitted per flower in 24 hr Clarkia breweri
2.1.1.273 0.0004518
-
 recombinant protein, with benzoic acid as substrate, pH 6.9, 20°C Clarkia breweri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.273 additional information no activity detected with salicylic acid and trans-cinnamic acid and their derivatives 3-hydroxybenzoic acid, 4-hydroxybenzoic acid, benzyl alcohol, and 2-,3- and 4-coumaric acid Clarkia breweri ?
-
 ?
2.1.1.273 S-adenosyl-L-methionine + benzoate emission of methyl benzoate occurs in a rhythmic manner, with maximum emission during the day, correlating with pollinator activity Clarkia breweri S-adenosyl-L-homocysteine + methyl benzoate
-
 ?
2.1.1.273 S-adenosyl-L-methionine + benzoate methyl benzoate is the most abundant scent compound detected in the majority of snapdragon varieties Clarkia breweri S-adenosyl-L-homocysteine + methyl benzoate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.273 BAMT
-
 Clarkia breweri
2.1.1.273 S-adenosyl-L-methionine:benzoic acid carboxyl methyl transferase
-
 Clarkia breweri

Expression

EC Number Organism Comment Expression
2.1.1.273 Clarkia breweri during flower life span, levels of methyl benzoate emission, BAMT activity, BAMT gene expression, and the amounts of BAMT protein and benzoic acid are developmentally and differentially regulated additional information

General Information

EC Number General Information Comment Organism
2.1.1.273 metabolism final enzyme in the biosynthesis of methyl benzoate Clarkia breweri
2.1.1.273 physiological function attracting pollinators like bumblebees Clarkia breweri