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Literature summary extracted from

  • Lin, J.; Mazarei, M.; Zhao, N.; Zhu, J.J.; Zhuang, X.; Liu, W.; Pantalone, V.R.; Arelli, P.R.; Stewart, C.N.; Chen, F.
    Overexpression of a soybean salicylic acid methyltransferase gene confers resistance to soybean cyst nematode (2013), Plant Biotechnol. J., 11, 1135-1145.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.274 expressed in Escherichia coli BL21(DE3) CodonPlus cells Glycine max

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.274 Ca2+ about 65% residual activity at 5 mM Glycine max
2.1.1.274 Cu2+ complete inhibition at 5 mM Glycine max
2.1.1.274 Fe2+ complete inhibition at 5 mM Glycine max
2.1.1.274 Fe3+ complete inhibition at 5 mM Glycine max
2.1.1.274 Mn2+ about 25% residual activity at 5 mM Glycine max
2.1.1.274 Zn2+ complete inhibition at 5 mM Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.274 0.00046
-
 salicylate in 50 mM Tris-HCl, at pH 7.5 and 25°C Glycine max

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.274 K+ 2fold increase in activity in the presence of 5 mM K+ Glycine max
2.1.1.274 additional information Na+, NH4+ and Mg2+ have no effect on isoform SAMT1 activity Glycine max

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.274 additional information Glycine max jasmonic acid, indole-3-acetic acid and gibberellic acid do not serve as substrates for isoform SAMT1 ?
-
 ?
2.1.1.274 S-adenosyl-L-methionine + 3-hydroxybenzoate Glycine max 1.8% activity compared to salicylate S-adenosyl-L-homocysteine + methyl 3-hydroxybenzoate
-
 ?
2.1.1.274 S-adenosyl-L-methionine + anthranilate Glycine max 9.3% activity compared to salicylate ?
-
 ?
2.1.1.274 S-adenosyl-L-methionine + benzoate Glycine max 16.9% activity compared to salicylate ?
-
 ?
2.1.1.274 S-adenosyl-L-methionine + salicylate Glycine max highest level of specific activity with salicylate S-adenosyl-L-homocysteine + methyl salicylate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.274 Glycine max
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.274 hairy root
-
 Glycine max
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.274 additional information jasmonic acid, indole-3-acetic acid and gibberellic acid do not serve as substrates for isoform SAMT1 Glycine max ?
-
 ?
2.1.1.274 S-adenosyl-L-methionine + 3-hydroxybenzoate 1.8% activity compared to salicylate Glycine max S-adenosyl-L-homocysteine + methyl 3-hydroxybenzoate
-
 ?
2.1.1.274 S-adenosyl-L-methionine + anthranilate 9.3% activity compared to salicylate Glycine max ?
-
 ?
2.1.1.274 S-adenosyl-L-methionine + benzoate 16.9% activity compared to salicylate Glycine max ?
-
 ?
2.1.1.274 S-adenosyl-L-methionine + salicylate highest level of specific activity with salicylate Glycine max S-adenosyl-L-homocysteine + methyl salicylate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.274 salicylic acid methyltransferase
-
 Glycine max
2.1.1.274 SAMT1
-
 Glycine max

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.274 25
-
-
 Glycine max

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.1.1.274 10 40 about 80% activity at 10°C, about 90% activity at 20°C, about 78% activity at 30°C, about 70% activity at 40°C Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.274 7.5
-
-
 Glycine max

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.274 7 8 about 90% activity at pH 7.0, about 40% activity at pH 8.0 Glycine max

General Information

EC Number General Information Comment Organism
2.1.1.274 physiological function isoform SAM1 plays a role in soybean defence against the soybean cyst nematode Heterodera glycines. Enzyme overexpression also affects the expression of selected genes involved in salicylic acid biosynthesis and salicylic acid signal transduction Glycine max