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Literature summary extracted from

  • Kovacs, F.A.; Sarath, G.; Woodworth, K.; Twigg, P.; Tobias, C.M.
    Abolishing activity against ascorbate in a cytosolic ascorbate peroxidase from switchgrass (2013), Phytochemistry, 94, 45-52.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.11 expression in Escherichia coli. Expression of holo enzyme depends on the simultaneous production of protein and heme by the bacteria Panicum virgatum

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.11 R172S negligible ascorbate peroxidase activity, but shows near wild type activity toward other aromatic substrates Panicum virgatum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.11 0.000043
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 7.0, temperature not specified in the publication. Hill coefficient n is 1.0 Panicum virgatum
1.11.1.11 0.804
-
 L-ascorbate pH 7.0, temperature not specified in the publication. Non-Michaelis-Menten kinetics, Hill ccoefficient n is 1.6 Panicum virgatum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.11.1.11 cytosol
-
 Panicum virgatum 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.11 26000
-
 1 * 26000, SDS-PAGE, His-tagged protein Panicum virgatum
1.11.1.11 29500
-
 gel filtration, His-tagged protein Panicum virgatum

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.11 Panicum virgatum
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.11 2 L-ascorbate + H2O2 + 2 H+
-
 Panicum virgatum 2 monodehydroascorbate + 2 H2O
-
 ?
1.11.1.11 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + H+
-
 Panicum virgatum ? + H2O
-
 ?
1.11.1.11 additional information guaiacol and pyrogallol are substrates, but the enzyme is inactivated by the oxidized guaiacol and pyrogallol products Panicum virgatum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.11 monomer 1 * 26000, SDS-PAGE, His-tagged protein Panicum virgatum

Synonyms

EC Number Synonyms Comment Organism
1.11.1.11 Pavirv00022559m
-
 Panicum virgatum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.11 0.4
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 7.0, temperature not specified in the publication. Hill coefficient n is 1.0 Panicum virgatum
1.11.1.11 161
-
 L-ascorbate pH 7.0, temperature not specified in the publication. Non-Michaelis-Menten kinetics, Hill ccoefficient n is 1.6 Panicum virgatum