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Literature summary extracted from

  • Watanabe, M.; Yumi, O.; Itoh, Y.; Yasuda, K.; Kamachi, K.; Ratcliffe, R.G.
    Deamination role of inducible glutamate dehydrogenase isoenzyme 7 in Brassica napus leaf protoplasts (2011), Phytochemistry, 72, 587-593.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.4.1.2 jasmonic acid
-
 Brassica napus
1.4.1.2 salicylic acid
-
 Brassica napus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.4.1.2 mitochondrion
-
 Brassica napus 5739
-
1.4.1.2 protoplast isozyme GDH7 predominates Brassica napus
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.2 L-glutamate + H2O + NAD+ Brassica napus
-
 2-oxoglutarate + NH3 + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.2 Brassica napus
-
 GDH isoenzymes 1 and 7, genes GDH1 and GDH2 encoding subunits alpha and beta
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.1.2 leaf the isoenzyme profile in leaves changes on wounding Brassica napus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.2 L-glutamate + H2O + NAD+
-
 Brassica napus 2-oxoglutarate + NH3 + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.4.1.2 hexamer (alphabeta)3 Brassica napus

Synonyms

EC Number Synonyms Comment Organism
1.4.1.2 NAD+-dependent GDH
-
 Brassica napus
1.4.1.2 NAD+-dependent glutamate dehydrogenase
-
 Brassica napus

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.2 NAD+
-
 Brassica napus
1.4.1.2 NADH
-
 Brassica napus

Expression

EC Number Organism Comment Expression
1.4.1.2 Brassica napus transcription of GDH2, which encodes the GDH alpha subunit, is activated and translation of the GDH2 mRNA is also activated to synthesize a subunit polypeptides, when protoplasts are isolated. When detached leaves absorb either acidic 5 mM jasmonic acid or salicylic acid solutions via petioles, GDH7 isoenzyme is activated and the GDH isoenzyme expression pattern is similar to that of protoplasts up

General Information

EC Number General Information Comment Organism
1.4.1.2 additional information the isoenzyme profile in leaves changes on wounding, the change in GDH isoenzyme profile has no effect on ammonium assimilation. Protoplast isolation changes the redox state with NAD(P)H and oxidized glutathione levels increasing, and ascorbate, dehydroascorbate, NAD(P)+ and glutathione decreasing. ATP content in protoplasts declines to 2.6% of that in leaves, while that in wounded leaves increases by twofold Brassica napus
1.4.1.2 physiological function isozyme GDH7 does not support net amination in vivo and the increase in GDH7 activity might be a response to oxidative stress during protoplast isolation Brassica napus