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Literature summary extracted from

  • Zhao, N.; Guan, J.; Lin, H.; Chen, F.
    Molecular cloning and biochemical characterization of indole-3-acetic acid methyltransferase from poplar (2007), Phytochemistry, 68, 1537-1544.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.278 expressed in Escherichia coli BL21 (DE3) CodonPlus cells Populus trichocarpa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.278 Cu2+ complete inhibition at 5 mM Populus trichocarpa
2.1.1.278 Fe2+ nearly complete inhibition at 5 mM Populus trichocarpa
2.1.1.278 Mg2+ about 80% residual activity at 5 mM Populus trichocarpa
2.1.1.278 Mn2+ about 50% residual activity at 5 mM Populus trichocarpa
2.1.1.278 Zn2+ nearly complete inhibition at 5 mM Populus trichocarpa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.278 0.0231
-
 S-adenosyl-L-methionine at pH 7.5 and 25°C Populus trichocarpa
2.1.1.278 0.0304
-
 (indol-3-yl)acetate at pH 7.5 and 25°C Populus trichocarpa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.278 Ca2+ mild activation at 5 mM Populus trichocarpa
2.1.1.278 K+ about 225% activity at 5 mM Populus trichocarpa
2.1.1.278 Na+ mild activation at 5 mM Populus trichocarpa
2.1.1.278 NH4+ mild activation at 5 mM Populus trichocarpa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.278 40000
-
 x * 40000, SDS-PAGE Populus trichocarpa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.278 S-adenosyl-L-methionine + (indol-3-yl)acetate Populus trichocarpa highest activity S-adenosyl-L-homocysteine + methyl (indol-3-yl)acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.278 Populus trichocarpa
-
 clone Nisqually-1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.278 Ni-NTA column agarose chromatography Populus trichocarpa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.278 leaf moderate expression in young leaves, no expression in old leaves Populus trichocarpa
-
2.1.1.278 root low level of expression Populus trichocarpa
-
2.1.1.278 stem highest level of expression Populus trichocarpa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.278 additional information no activity with salicylic acid, benzoic acid, jasmonic acid, and farnesoic acid Populus trichocarpa ?
-
 ?
2.1.1.278 S-adenosyl-L-methionine + (indol-3-yl)acetate highest activity Populus trichocarpa S-adenosyl-L-homocysteine + methyl (indol-3-yl)acetate
-
 ?
2.1.1.278 S-adenosyl-L-methionine + (indol-3-yl)butyrate 11% activity compared to (indol-3-yl)acetate Populus trichocarpa S-adenosyl-L-homocysteine + methyl (indol-3-yl)butyrate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.278 ? x * 40000, SDS-PAGE Populus trichocarpa

Synonyms

EC Number Synonyms Comment Organism
2.1.1.278 IAMT
-
 Populus trichocarpa
2.1.1.278 indole-3-acetic acid methyltransferase
-
 Populus trichocarpa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.278 25
-
-
 Populus trichocarpa

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.1.1.278 4 40 activity at 4°C and 40°C is about 40% of the maximal activity Populus trichocarpa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.278 7.5
-
-
 Populus trichocarpa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.278 6.7 9 at pH 6.5, the enzyme has about 34% of its maximal activity, whereas at pH 9.0, the enzyme displays about 40% of the maximal activity Populus trichocarpa

General Information

EC Number General Information Comment Organism
2.1.1.278 physiological function the enzyme is involved in development of root and leaf in poplar and plays a role in wood formation Populus trichocarpa

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1.1.278 4.782
-
 S-adenosyl-L-methionine at pH 7.5 and 25°C Populus trichocarpa