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Literature summary extracted from

  • Singh, P.; Tiwari, A.; Singh, S.; Asthana, R.
    Proline biosynthesizing enzymes (glutamate 5-kinase and pyrroline-5-carboxylate reductase) from a model cyanobacterium for desiccation tolerance (2013), Physiol. Mol. Biol. Plants, 19, 521-528.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.2 expressed in Escherichia coli BL21(DE3) cells Anabaena sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.2 28140
-
 x * 28140, calculated from SDS-PAGE Anabaena sp.

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.2 Anabaena sp.
-
-
-
1.5.1.2 Anabaena sp. PCC 7120
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.2 Ni+2-NTA His-Trap resin column chromatography, and gel filtration Anabaena sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.2 1-pyrroline-5-carboxylate + NAD(P)H + H+
-
 Anabaena sp. L-proline + NAD(P)+
-
 ?
1.5.1.2 1-pyrroline-5-carboxylate + NAD(P)H + H+
-
 Anabaena sp. PCC 7120 L-proline + NAD(P)+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.2 ? x * 28140, calculated from SDS-PAGE Anabaena sp.

Synonyms

EC Number Synonyms Comment Organism
1.5.1.2 P5CR
-
 Anabaena sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.2 NADH
-
 Anabaena sp.
1.5.1.2 NADPH
-
 Anabaena sp.