BRENDA - Enzyme Database

High performance enzyme fuel cells using a genetically expressed FAD-dependent glucose dehydrogenase alpha-subunit of Burkholderia cepacia immobilized in a carbon nanotube electrode for low glucose conditions

Fapyane, D.; Lee, S.J.; Kang, S.H.; Lim, D.H.; Cho, K.K.; Nam, T.H.; Ahn, J.P.; Ahn, J.H.; Kim, S.W.; Chang, I.S.; Phys. Chem. Chem. Phys. 15, 9508-9512 (2013)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.1.5.9
expression in Escherichia coli strain BL21RIL, no GDH activity when cells expressing the alpha-subunit alone
Burkholderia cepacia
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.1.5.9
additional information
the purified recombinnat enzyme is used as an anode catalyst for enzyme fuel cells, method, overview. The recombinant FAD-GDH is able to maintain its native glucose affinity during immobilization in the carbon nanotube and operation of enzyme fuel cells. Heterogeneous electron transfer coefficient of FAD-GDH–menadione on a glassy carbon electrode was 10.73/s
Burkholderia cepacia
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.5.9
0.4638
-
D-glucose
pH and temperature not specified in the publication
Burkholderia cepacia
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.5.9
18000
-
1 * 59800 + 1 * 18000, SDS-PAGE
Burkholderia cepacia
1.1.5.9
59800
-
1 * 59800 + 1 * 18000, SDS-PAGE
Burkholderia cepacia
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.5.9
D-glucose + a quinone
Burkholderia cepacia
-
D-glucono-1,5-lactone + a quinol
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.5.9
Burkholderia cepacia
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.5.9
recombinant enzyme from Escherichia coli strain BL21RIL
Burkholderia cepacia
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.5.9
D-glucose + a quinone
-
726109
Burkholderia cepacia
D-glucono-1,5-lactone + a quinol
-
-
-
?
1.1.5.9
D-glucose + menadione
-
726109
Burkholderia cepacia
D-glucono-1,5-lactone + menadiol
-
-
-
?
1.1.5.9
D-glucose + oxidized 2,6-dichlorophenol indophenol
-
726109
Burkholderia cepacia
D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.5.9
dimer
1 * 59800 + 1 * 18000, SDS-PAGE
Burkholderia cepacia
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1.5.9
69.54
-
D-glucose
pH and temperature not specified in the publication
Burkholderia cepacia
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.5.9
FAD
dependent on, bound in the catalytic site
Burkholderia cepacia
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.5.9
expression in Escherichia coli strain BL21RIL, no GDH activity when cells expressing the alpha-subunit alone
Burkholderia cepacia
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.5.9
FAD
dependent on, bound in the catalytic site
Burkholderia cepacia
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.1.5.9
additional information
the purified recombinnat enzyme is used as an anode catalyst for enzyme fuel cells, method, overview. The recombinant FAD-GDH is able to maintain its native glucose affinity during immobilization in the carbon nanotube and operation of enzyme fuel cells. Heterogeneous electron transfer coefficient of FAD-GDH–menadione on a glassy carbon electrode was 10.73/s
Burkholderia cepacia
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.5.9
0.4638
-
D-glucose
pH and temperature not specified in the publication
Burkholderia cepacia
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.5.9
18000
-
1 * 59800 + 1 * 18000, SDS-PAGE
Burkholderia cepacia
1.1.5.9
59800
-
1 * 59800 + 1 * 18000, SDS-PAGE
Burkholderia cepacia
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.5.9
D-glucose + a quinone
Burkholderia cepacia
-
D-glucono-1,5-lactone + a quinol
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.5.9
recombinant enzyme from Escherichia coli strain BL21RIL
Burkholderia cepacia
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.5.9
D-glucose + a quinone
-
726109
Burkholderia cepacia
D-glucono-1,5-lactone + a quinol
-
-
-
?
1.1.5.9
D-glucose + menadione
-
726109
Burkholderia cepacia
D-glucono-1,5-lactone + menadiol
-
-
-
?
1.1.5.9
D-glucose + oxidized 2,6-dichlorophenol indophenol
-
726109
Burkholderia cepacia
D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.5.9
dimer
1 * 59800 + 1 * 18000, SDS-PAGE
Burkholderia cepacia
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1.5.9
69.54
-
D-glucose
pH and temperature not specified in the publication
Burkholderia cepacia