EC Number | Crystallization (Comment) | Organism |
---|---|---|
4.1.2.13 | sitting-drop vapour-diffusion method at 25°C, crystallization of the enzyme in the presence of glycerone phosphate and Mg2+, determination of the crystal structure at 1.5 A resolution. Crystal structure of the bifunctional enzyme (fructose-bisphosphate aldolase/fructose-bisphosphatase) at 1.5 A resolution in the aldolase form, where a critical lysine residue forms a Schiff base with glycerone phosphate. A structural comparison of the aldolase form with a phosphatase form reveals a dramatic conformational change in the active site, demonstrating that the enzyme metamorphoses its active-site architecture to exhibit dual activities | Sulfurisphaera tokodaii |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.3.11 | Y229F | the kcat/Km-value of the bifunctional enzyme (EC 3.1.3.11/EC 4.1.2.13) for D-fructose 1,6-bisphosphate is 1.1fold higher than the wild-type value, no fructose-bisphosphate aldolase activity | Sulfurisphaera tokodaii |
3.1.3.11 | Y348F | the kcat/Km-value of the bifunctional enzyme (EC 3.1.3.11/EC 4.1.2.13) for D-fructose 1,6-bisphosphate is 3.5fold lower than the wild-type value, the kcat/Km-value for the fructose-bisphosphate aldolase reaction of the bifunctional enzyme (EC 3.1.3.11/EC 4.1.2.13) in the anabolic direction is 16fold lower than wild-type value | Sulfurisphaera tokodaii |
4.1.2.13 | Y229F | the kcat/Km-value of the bifunctional enzyme (EC 3.1.3.11/EC 4.1.2.13) for D-fructose 1,6-bisphosphate is 1.1fold higher than the wild-type value, no fructose-bisphosphate aldolase activity | Sulfurisphaera tokodaii |
4.1.2.13 | Y348F | the kcat/Km-value of the bifunctional enzyme (EC 3.1.3.11/EC 4.1.2.13) for D-fructose 1,6-bisphosphate is 3.5fold lower than the wild-type value, the kcat/Km-value for the fructose-bisphosphate aldolase reaction of the bifunctional enzyme (EC 3.1.3.11/EC 4.1.2.13) in the anabolic direction is 16fold lower than wild-type value | Sulfurisphaera tokodaii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.11 | 0.027 | - |
D-fructose 1,6-bisphosphate | pH 7.8, 48°C, mutant enzyme Y229F | Sulfurisphaera tokodaii | |
3.1.3.11 | 0.027 | - |
D-fructose 1,6-bisphosphate | pH 7.8, 48°C, wild-type enzyme | Sulfurisphaera tokodaii | |
3.1.3.11 | 0.036 | - |
D-fructose 1,6-bisphosphate | pH 7.8, 48°C, mutant enzyme Y348F | Sulfurisphaera tokodaii | |
4.1.2.13 | 0.19 | - |
D-glyceraldehyde 3-phosphate | pH 8.0, 48°C, wild-type enzyme | Sulfurisphaera tokodaii | |
4.1.2.13 | 0.19 | - |
glycerone phosphate | pH 8.0, 48°C, wild-type enzyme | Sulfurisphaera tokodaii | |
4.1.2.13 | 0.34 | - |
D-glyceraldehyde 3-phosphate | pH 8.0, 48°C, mutant enzyme Y348F | Sulfurisphaera tokodaii | |
4.1.2.13 | 0.34 | - |
glycerone phosphate | pH 8.0, 48°C, mutant enzyme Y348F | Sulfurisphaera tokodaii |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.1.2.13 | Mg2+ | the glycerone phosphate group coordinates three Mg2+ ions (Mg2Mg4) | Sulfurisphaera tokodaii |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.11 | Sulfurisphaera tokodaii | F9VMT6 | - |
- |
3.1.3.11 | Sulfurisphaera tokodaii 7 | F9VMT6 | - |
- |
4.1.2.13 | Sulfurisphaera tokodaii | F9VMT6 | - |
- |
4.1.2.13 | Sulfurisphaera tokodaii 7 | F9VMT6 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.11 | D-fructose 1,6-bisphosphate + H2O | the bifunctional enzyme also shows activity of EC 4.1.2.13. The crystal structures of the enzyme in the two forms reveals that it achieves its bifunctionality by transforming its active-site architecture, through the toggle switch-like motions of the three mobile loop regions | Sulfurisphaera tokodaii | D-fructose 6-phosphate + phosphate | - |
r | |
3.1.3.11 | D-fructose 1,6-bisphosphate + H2O | the bifunctional enzyme also shows activity of EC 4.1.2.13. The crystal structures of the enzyme in the two forms reveals that it achieves its bifunctionality by transforming its active-site architecture, through the toggle switch-like motions of the three mobile loop regions | Sulfurisphaera tokodaii 7 | D-fructose 6-phosphate + phosphate | - |
r | |
4.1.2.13 | Glycerone phosphate + D-glyceraldehyde 3-phosphate | the bifunctional enzyme also shows activity of EC 3.1.3.11. The crystal structures of the enzyme in the two forms reveals that it achieves its bifunctionality by transforming its active-site architecture, through the toggle switch-like motions of the three mobile loop regions | Sulfurisphaera tokodaii | D-Fructose 1,6-bisphosphate | - |
r | |
4.1.2.13 | Glycerone phosphate + D-glyceraldehyde 3-phosphate | the bifunctional enzyme also shows activity of EC 3.1.3.11. The crystal structures of the enzyme in the two forms reveals that it achieves its bifunctionality by transforming its active-site architecture, through the toggle switch-like motions of the three mobile loop regions | Sulfurisphaera tokodaii 7 | D-Fructose 1,6-bisphosphate | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.3.11 | FBP aldolase/phosphatase | bifunctional enzyme EC 3.1.3.11/EC 4.1.2.13 | Sulfurisphaera tokodaii |
3.1.3.11 | FBPA/P | bifunctional enzyme EC 3.1.3.11/EC 4.1.2.13 | Sulfurisphaera tokodaii |
3.1.3.11 | fructose-1,6-bisphosphate aldolase/phosphatase | bifunctional enzyme EC 3.1.3.11/EC 4.1.2.13 | Sulfurisphaera tokodaii |
3.1.3.11 | STK_03180 | locus name. The bifunctional enzyme also shows activity of EC 4.1.2.13 | Sulfurisphaera tokodaii |
4.1.2.13 | FBP aldolase/phosphatase | bifunctional enzyme EC 3.1.3.11/EC 4.1.2.13 | Sulfurisphaera tokodaii |
4.1.2.13 | FBPA/P | bifunctional enzyme EC 3.1.3.11/EC 4.1.2.13 | Sulfurisphaera tokodaii |
4.1.2.13 | fructose-1,6-bisphosphate aldolase/phosphatase | bifunctional enzyme EC 3.1.3.11/EC 4.1.2.13 | Sulfurisphaera tokodaii |
4.1.2.13 | STK_03180 | locus name. The bifunctional enzyme also shows activity of EC 3.1.3.11 | Sulfurisphaera tokodaii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.11 | 48 | - |
assay at | Sulfurisphaera tokodaii |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.11 | 0.26 | - |
D-fructose 1,6-bisphosphate | pH 7.8, 48°C, mutant enzyme Y348F | Sulfurisphaera tokodaii | |
3.1.3.11 | 0.62 | - |
D-fructose 1,6-bisphosphate | pH 7.8, 48°C, wild-type enzyme | Sulfurisphaera tokodaii | |
3.1.3.11 | 0.66 | - |
D-fructose 1,6-bisphosphate | pH 7.8, 48°C, mutant enzyme Y229F | Sulfurisphaera tokodaii | |
4.1.2.13 | 0.026 | - |
D-fructose 1,6-bisphosphate | pH 8.0, 48°C, mutant enzyme Y348F | Sulfurisphaera tokodaii | |
4.1.2.13 | 0.027 | - |
D-fructose 1,6-bisphosphate | pH 8.0, 48°C, wild-type enzyme | Sulfurisphaera tokodaii | |
4.1.2.13 | 0.1 | - |
D-glyceraldehyde 3-phosphate | pH 8.0, 48°C, mutant enzyme Y348F | Sulfurisphaera tokodaii | |
4.1.2.13 | 0.1 | - |
glycerone phosphate | pH 8.0, 48°C, mutant enzyme Y348F | Sulfurisphaera tokodaii | |
4.1.2.13 | 0.91 | - |
D-glyceraldehyde 3-phosphate | pH 8.0, 48°C, wild-type enzyme | Sulfurisphaera tokodaii | |
4.1.2.13 | 0.91 | - |
glycerone phosphate | pH 8.0, 48°C, wild-type enzyme | Sulfurisphaera tokodaii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.11 | 7.8 | - |
assay at | Sulfurisphaera tokodaii |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.11 | 7.2 | - |
D-fructose 1,6-bisphosphate | pH 7.8, 48°C, mutant enzyme Y348F | Sulfurisphaera tokodaii | |
3.1.3.11 | 23 | - |
D-fructose 1,6-bisphosphate | pH 7.8, 48°C, wild-type enzyme | Sulfurisphaera tokodaii | |
3.1.3.11 | 25 | - |
D-fructose 1,6-bisphosphate | pH 7.8, 48°C, mutant enzyme Y229F | Sulfurisphaera tokodaii | |
4.1.2.13 | 0.29 | - |
D-glyceraldehyde 3-phosphate | pH 8.0, 48°C, mutant enzyme Y348F | Sulfurisphaera tokodaii | |
4.1.2.13 | 0.29 | - |
glycerone phosphate | pH 8.0, 48°C, mutant enzyme Y348F | Sulfurisphaera tokodaii | |
4.1.2.13 | 4.7 | - |
D-glyceraldehyde 3-phosphate | pH 8.0, 48°C, wild-type enzyme | Sulfurisphaera tokodaii | |
4.1.2.13 | 4.7 | - |
glycerone phosphate | pH 8.0, 48°C, wild-type enzyme | Sulfurisphaera tokodaii |