EC Number | Cloned (Comment) | Organism |
---|---|---|
2.8.2.37 | Stf0 gene is amplified from Mycobacterium tuberculosis H37Rv and expressed in Escherichia coli | Mycobacterium tuberculosis |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.8.2.37 | vapor diffusion method, crystal structure of the enzyme bound to trehalose | Mycolicibacterium smegmatis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.8.2.37 | E33A | kcat/Km for trehalose is 4.2fold lower compared to wild-type value | Mycolicibacterium smegmatis |
2.8.2.37 | E36A | kcat/Km for trehalose is 34.2fold lower compared to wild-type value | Mycolicibacterium smegmatis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.8.2.37 | 6.4 | - |
alpha,alpha-trehalose | pH 7.5, 22°C, mutant enzyme E36Q | Mycolicibacterium smegmatis | |
2.8.2.37 | 18 | - |
alpha,alpha-trehalose | pH 7.5, 22°C, wild-type enzyme | Mycolicibacterium smegmatis | |
2.8.2.37 | 29 | - |
alpha,alpha-trehalose | pH 7.5, 22°C, mutant enzyme E33A | Mycolicibacterium smegmatis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.8.2.37 | 3'-phosphoadenylyl sulfate + alpha,alpha-trehalose | Mycobacterium tuberculosis | sulfotransferase Stf0 carries out the first committed step in the biosynthesis of sulfolipid SL-1 | adenosine 3',5'-bisphosphate + 2-O-sulfo-alpha,alpha-trehalose | - |
? | |
2.8.2.37 | 3'-phosphoadenylyl sulfate + alpha,alpha-trehalose | Mycolicibacterium smegmatis | sulfotransferase Stf0 carries out the first committed step in the biosynthesis of sulfolipid SL-1 | adenosine 3',5'-bisphosphate + 2-O-sulfo-alpha,alpha-trehalose | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.8.2.37 | Mycobacterium tuberculosis | O53699 | - |
- |
2.8.2.37 | Mycolicibacterium smegmatis | P84151 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.8.2.37 | - |
Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.8.2.37 | 3'-phosphoadenylyl sulfate + alpha,alpha-trehalose | - |
Mycobacterium tuberculosis | adenosine 3',5'-bisphosphate + 2-O-sulfo-alpha,alpha-trehalose | - |
? | |
2.8.2.37 | 3'-phosphoadenylyl sulfate + alpha,alpha-trehalose | - |
Mycolicibacterium smegmatis | adenosine 3',5'-bisphosphate + 2-O-sulfo-alpha,alpha-trehalose | - |
? | |
2.8.2.37 | 3'-phosphoadenylyl sulfate + alpha,alpha-trehalose | sulfotransferase Stf0 carries out the first committed step in the biosynthesis of sulfolipid SL-1 | Mycobacterium tuberculosis | adenosine 3',5'-bisphosphate + 2-O-sulfo-alpha,alpha-trehalose | - |
? | |
2.8.2.37 | 3'-phosphoadenylyl sulfate + alpha,alpha-trehalose | sulfotransferase Stf0 carries out the first committed step in the biosynthesis of sulfolipid SL-1 | Mycolicibacterium smegmatis | adenosine 3',5'-bisphosphate + 2-O-sulfo-alpha,alpha-trehalose | - |
? | |
2.8.2.37 | 3'-phosphoadenylyl sulfate + alpha-D-galactopyranosyl alpha-D-glucopyranoside | alpha-D-galactopyranosyl-alpha-D-glucopyranoside is a synthetic analog of trehalose epimerized at a single stereocenter is sulfated 68fold less efficiently than trehalose | Mycolicibacterium smegmatis | ? | - |
? | |
2.8.2.37 | additional information | no activity on beta-phenyl glucoside, glucose, neo-trehalose or iso-trehalose | Mycolicibacterium smegmatis | ? | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.8.2.37 | 0.017 | - |
alpha,alpha-trehalose | pH 7.5, 22°C, mutant enzyme E36Q | Mycolicibacterium smegmatis | |
2.8.2.37 | 0.61 | - |
alpha,alpha-trehalose | pH 7.5, 22°C, mutant enzyme E33A | Mycolicibacterium smegmatis | |
2.8.2.37 | 1.6 | - |
alpha,alpha-trehalose | pH 7.5, 22°C, wild-type enzyme | Mycolicibacterium smegmatis |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.8.2.37 | malfunction | Mycobacterium tuberculosis deficient in Stf0 lacks both sulfolipid SL-1 and partially modified analogs | Mycobacterium tuberculosis |
2.8.2.37 | physiological function | sulfotransferase Stf0 carries out the first committed step in the biosynthesis of sulfolipid SL-1 | Mycobacterium tuberculosis |
2.8.2.37 | physiological function | sulfotransferase Stf0 carries out the first committed step in the biosynthesis of sulfolipid SL-1 | Mycolicibacterium smegmatis |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.8.2.37 | 0.0026 | - |
alpha,alpha-trehalose | pH 7.5, 22°C, mutant enzyme E36Q | Mycolicibacterium smegmatis | |
2.8.2.37 | 0.021 | - |
alpha,alpha-trehalose | pH 7.5, 22°C, mutant enzyme E33A | Mycolicibacterium smegmatis | |
2.8.2.37 | 0.89 | - |
alpha,alpha-trehalose | pH 7.5, 22°C, wild-type enzyme | Mycolicibacterium smegmatis |