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Literature summary extracted from

  • Horie, A.; Tomita, T.; Saiki, A.; Kono, H.; Taka, H.; Mineki, R.; Fujimura, T.; Nishiyama, C.; Kuzuyama, T.; Nishiyama, M.
    Discovery of proteinaceous N-modification in lysine biosynthesis of Thermus thermophilus (2009), Nat. Chem. Biol., 5, 673-679.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.103 expressed in Escherichia coli Thermus thermophilus
3.5.1.130 expression in Escherichia coli Thermus thermophilus
6.3.2.43 expression in Escherichia coli BL21 Thermus thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.130 [carrier protein LysW]-C-terminal-alpha-(L-lysyl)-L-glutamate
-
 Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.43 0.078
-
 [LysW]-C-terminal-L-glutamate54 pH 8.0, 60°C Thermus thermophilus
6.3.2.43 2.1
-
 L-2-aminoadipate pH 8.0, 60°C Thermus thermophilus
6.3.2.43 2.6
-
 ATP pH 8.0, 60°C Thermus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.103 Co2+ 0.01 mM used in assay conditions Thermus thermophilus
1.2.1.103 Mg2+ 0.01 mM used in assay conditions Thermus thermophilus
1.2.1.103 Zn2+ 0.01 mM used in assay conditions Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.103 an [amino group carrier protein]-C-terminal-N-(1-carboxy-5-phosphooxy-5-oxopentyl)-L-glutamine + NADPH + H+ Thermus thermophilus
-
 an [amino group carrier protein]-C-terminal-N-(1-carboxy-5-oxopentyl)-L-glutamine + phosphate + NADP+
-
 r
2.7.2.17 ATP + an [amino group carrier protein]-C-terminal-N-(1,4-dicarboxybutyl)-L-glutamine Thermus thermophilus the enzyme participates in an L-lysine biosynthetric pathway ADP + phosphate + an [amino group carrier protein]-C-terminal-N-(1-carboxy-5-phosphooxy-5-oxopentyl)-L-glutamine
-
 ?
3.5.1.130 additional information Thermus thermophilus for the conversion of alpha-aminoadipate to lysine, the amino group of alpha-aminoadipate is modified by attachment to the gamma-carboxyl group of the C-terminal Glu54 of a small protein, LysW. The side chain of alpha-aminoadipate is converted to the lysyl side chain while still attached to LysW, and lysine is subsequently liberated from the LysW-lysine fusion ?
-
 ?
3.5.1.130 additional information Thermus thermophilus DSM 7039 for the conversion of alpha-aminoadipate to lysine, the amino group of alpha-aminoadipate is modified by attachment to the gamma-carboxyl group of the C-terminal Glu54 of a small protein, LysW. The side chain of alpha-aminoadipate is converted to the lysyl side chain while still attached to LysW, and lysine is subsequently liberated from the LysW-lysine fusion ?
-
 ?
3.5.1.130 [carrier protein LysW]-C-terminal-gamma-(L-lysyl)-L-glutamate + H2O Thermus thermophilus
-
 [carrier protein LysW]-C-terminal-L-glutamate + L-lysine
-
 ?
3.5.1.130 [carrier protein LysW]-C-terminal-gamma-(L-lysyl)-L-glutamate + H2O Thermus thermophilus DSM 7039
-
 [carrier protein LysW]-C-terminal-L-glutamate + L-lysine
-
 ?
6.3.2.43 ATP + [LysW]-C-terminal-L-glutamate54 + L-2-aminoadipate Thermus thermophilus the enzyme is involved in biosynthesis of lysine. LysX activates the gamma-carboxyl group of the C-terminal Glu54 of the 2-L-aminoaadipate carrier-protein LysW by phosphorylation, with concomitant conversion of ATP to ADP. LysX recognizes not only the C-terminal glutamate residue of LysW but also other portions, possibly the globular domain of LysW specifically ADP + phosphate + [LysW]-C-terminal-gamma-(L-2-aminoadip-2-yl)-L-glutamate54
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.103 Thermus thermophilus
-
-
-
2.6.1.118 Thermus thermophilus Q93R93
-
-
2.7.2.17 Thermus thermophilus
-
-
-
3.5.1.130 Thermus thermophilus Q8VUS5
-
-
3.5.1.130 Thermus thermophilus DSM 7039 Q8VUS5
-
-
6.3.2.43 Thermus thermophilus Q5SH23
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.130 recombinant protein Thermus thermophilus
6.3.2.43
-
 Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.103 an [amino group carrier protein]-C-terminal-N-(1-carboxy-5-phosphooxy-5-oxopentyl)-L-glutamine + NADPH + H+
-
 Thermus thermophilus an [amino group carrier protein]-C-terminal-N-(1-carboxy-5-oxopentyl)-L-glutamine + phosphate + NADP+
-
 r
2.6.1.118 [carrier protein LysW]-C-terminal-N-(1-carboxy-5-oxopentan-1-yl)-L-glutamine + L-glutamate
-
 Thermus thermophilus [carrier protein LysW]-C-terminal-gamma-(L-lysyl)-L-glutamate + 2-oxoglutarate
-
 ?
2.7.2.17 ATP + an [amino group carrier protein]-C-terminal-N-(1,4-dicarboxybutyl)-L-glutamine the enzyme participates in an L-lysine biosynthetric pathway Thermus thermophilus ADP + phosphate + an [amino group carrier protein]-C-terminal-N-(1-carboxy-5-phosphooxy-5-oxopentyl)-L-glutamine
-
 ?
2.7.2.17 ATP + an [amino group carrier protein]-C-terminal-N-(1,4-dicarboxybutyl)-L-glutamine
-
 Thermus thermophilus ADP + phosphate + an [amino group carrier protein]-C-terminal-N-(1-carboxy-5-phosphooxy-5-oxopentyl)-L-glutamine
-
 ?
3.5.1.130 additional information for the conversion of alpha-aminoadipate to lysine, the amino group of alpha-aminoadipate is modified by attachment to the gamma-carboxyl group of the C-terminal Glu54 of a small protein, LysW. The side chain of alpha-aminoadipate is converted to the lysyl side chain while still attached to LysW, and lysine is subsequently liberated from the LysW-lysine fusion Thermus thermophilus ?
-
 ?
3.5.1.130 additional information no substrates: [carrier protein LysW]-C-terminal-alpha-(L-lysyl)-L-glutamate, [carrier protein LysW]-13-alpha-(L-lysyl)-L-glutamate Thermus thermophilus ?
-
 ?
3.5.1.130 additional information for the conversion of alpha-aminoadipate to lysine, the amino group of alpha-aminoadipate is modified by attachment to the gamma-carboxyl group of the C-terminal Glu54 of a small protein, LysW. The side chain of alpha-aminoadipate is converted to the lysyl side chain while still attached to LysW, and lysine is subsequently liberated from the LysW-lysine fusion Thermus thermophilus DSM 7039 ?
-
 ?
3.5.1.130 additional information no substrates: [carrier protein LysW]-C-terminal-alpha-(L-lysyl)-L-glutamate, [carrier protein LysW]-13-alpha-(L-lysyl)-L-glutamate Thermus thermophilus DSM 7039 ?
-
 ?
3.5.1.130 [carrier protein LysW]-C-terminal-gamma-(L-lysyl)-L-glutamate + H2O
-
 Thermus thermophilus [carrier protein LysW]-C-terminal-L-glutamate + L-lysine
-
 ?
3.5.1.130 [carrier protein LysW]-C-terminal-gamma-(L-lysyl)-L-glutamate + H2O
-
 Thermus thermophilus DSM 7039 [carrier protein LysW]-C-terminal-L-glutamate + L-lysine
-
 ?
6.3.2.43 ATP + [LysW]-C-terminal-L-glutamate54 + L-2-aminoadipate the enzyme is involved in biosynthesis of lysine. LysX activates the gamma-carboxyl group of the C-terminal Glu54 of the 2-L-aminoaadipate carrier-protein LysW by phosphorylation, with concomitant conversion of ATP to ADP. LysX recognizes not only the C-terminal glutamate residue of LysW but also other portions, possibly the globular domain of LysW specifically Thermus thermophilus ADP + phosphate + [LysW]-C-terminal-gamma-(L-2-aminoadip-2-yl)-L-glutamate54
-
 ?
6.3.2.43 ATP + [LysW]-C-terminal-L-glutamate54 + L-2-aminoadipate LysX activates the gamma-carboxyl group of the C-terminal Glu54 of the 2-L-aminoaadipate carrier-protein LysW by phosphorylation, with concomitant conversion of ATP to ADP. LysX recognizes not only the C-terminal glutamate residue of LysW but also other portions, possibly the globular domain of LysW specifically Thermus thermophilus ADP + phosphate + [LysW]-C-terminal-gamma-(L-2-aminoadip-2-yl)-L-glutamate54
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.103 ? x * about 40000, SDS-PAGE Thermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
1.2.1.103 lysY
-
 Thermus thermophilus
6.3.2.43 alpha-aminoadipate-lysW ligase lysX
-
 Thermus thermophilus
6.3.2.43 LysX
-
 Thermus thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.2.43 0.85
-
 L-2-aminoadipate pH 8.0, 60°C Thermus thermophilus
6.3.2.43 2
-
 [LysW]-C-terminal-L-glutamate54 pH 8.0, 60°C Thermus thermophilus
6.3.2.43 5.2
-
 ATP pH 8.0, 60°C Thermus thermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.103 ATP 20 mM used in assay conditions Thermus thermophilus
1.2.1.103 NADP+
-
 Thermus thermophilus
1.2.1.103 NADPH
-
 Thermus thermophilus
1.2.1.103 pyridoxal 5'-phosphate 5 mM used in assay conditions Thermus thermophilus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.5.1.130 0.00067
-
 pH 8.0, 60°C Thermus thermophilus [carrier protein LysW]-C-terminal-alpha-(L-lysyl)-L-glutamate

General Information

EC Number General Information Comment Organism
2.6.1.118 physiological function for the conversion of alpha-aminoadipate to lysine, the amino group of alpha-aminoadipate is modified by attachment to the gamma-carboxyl group of the C-terminal Glu54 of a small protein, LysW. The side chain of alpha-aminoadipate is converted to the lysyl side chain while still attached to LysW, and lysine is subsequently liberated from the LysW-lysine fusion Thermus thermophilus
2.7.2.17 physiological function the enzyme participates in an L-lysine biosynthetic pathway Thermus thermophilus
6.3.2.43 physiological function the enzyme is involved in biosynthesis of lysine in Thermus thermophilus Thermus thermophilus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.3.2.43 0.4
-
 L-2-aminoadipate pH 8.0, 60°C Thermus thermophilus
6.3.2.43 2.7
-
 ATP pH 8.0, 60°C Thermus thermophilus
6.3.2.43 26
-
 [LysW]-C-terminal-L-glutamate54 pH 8.0, 60°C Thermus thermophilus