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Literature summary extracted from

  • Carlson, K.; Kosturko, L.D.; Nystrom, A.C.
    Sequence-specific cleavage by bacteriophage T4 endonuclease II in vitro (1999), Mol. Microbiol., 31, 1395-1405.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.21.8
-
 Tequatrovirus T4

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.21.8 Mg2+ above 10 mM Tequatrovirus T4
3.1.21.8 NaCl above 50 mM Tequatrovirus T4

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.21.8 Mg2+ required Tequatrovirus T4

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.21.8 DNA + H2O Tequatrovirus T4 the phage T4 enzyme is involved in degradation of host DNA ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.21.8 Tequatrovirus T4 P07059
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.21.8 DNA + H2O the phage T4 enzyme is involved in degradation of host DNA Tequatrovirus T4 ?
-
 ?
3.1.21.8 DNA + H2O the phage T4 enzyme is involved in degradation of host DNA. The enzyme primarily catalyses nicking of the bottom strand of double stranded DNA between the first and second base pair to the right of a top-strand CCGC motif. Double-stranded breaks are produced 5fold to 10fold less frequently. It does not cleave the T4 native DNA, which contains 5-hydroxymethylcytosine instead of cytosine Tequatrovirus T4 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.21.8 denA
-
 Tequatrovirus T4
3.1.21.8 EndoII ambiguous Tequatrovirus T4
3.1.21.8 T4 endonuclease II
-
 Tequatrovirus T4

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.21.8 30 37 activity at 30°C is somewhat lower than at 37°C Tequatrovirus T4

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.21.8 37
-
 enzyme remains active for at least 120 min Tequatrovirus T4

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.21.8 7 8
-
 Tequatrovirus T4

General Information

EC Number General Information Comment Organism
3.1.21.8 physiological function the phage T4 enzyme is involved in degradation of host DNA Tequatrovirus T4