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Literature summary extracted from

  • Kim, T.H.; Kim, D.H.; Nam, H.W.; Park, S.Y.; Shim, J.; Cho, J.W.
    Tyrosylprotein sulfotransferase regulates collagen secretion in Caenorhabditis elegans (2010), Mol. Cells, 29, 413-418.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.2.20 expressed in HEK-293 cells Caenorhabditis elegans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.8.2.20 Golgi apparatus the enzyme is localized to the trans-Golgi network Caenorhabditis elegans 5794
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.2.20 47000
-
 x * 47000, SDS-PAGE Caenorhabditis elegans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.2.20 3'-phosphoadenylyl sulfate + ROL-6 protein tyrosine Caenorhabditis elegans the enzyme sulfates three tyrosine residues of ROL-6 protein in vitro adenosine 3',5'-bisphosphate + ROL-6 protein tyrosine-O-sulfate
-
 ?
2.8.2.20 3'-phosphoadenylyl sulfate + ROL-6 protein tyrosine Caenorhabditis elegans N2 the enzyme sulfates three tyrosine residues of ROL-6 protein in vitro adenosine 3',5'-bisphosphate + ROL-6 protein tyrosine-O-sulfate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.20 Caenorhabditis elegans
-
-
-
2.8.2.20 Caenorhabditis elegans N2
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.20 Ni-NTA agarose bead chromatography, and gel filtration Caenorhabditis elegans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.20 3'-phosphoadenylyl sulfate + ROL-6 protein tyrosine the enzyme sulfates three tyrosine residues of ROL-6 protein in vitro Caenorhabditis elegans adenosine 3',5'-bisphosphate + ROL-6 protein tyrosine-O-sulfate
-
 ?
2.8.2.20 3'-phosphoadenylyl sulfate + ROL-6 protein tyrosine the enzyme sulfates three tyrosine residues of ROL-6 protein in vitro Caenorhabditis elegans N2 adenosine 3',5'-bisphosphate + ROL-6 protein tyrosine-O-sulfate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.2.20 ? x * 47000, SDS-PAGE Caenorhabditis elegans

Synonyms

EC Number Synonyms Comment Organism
2.8.2.20 TPST-1
-
 Caenorhabditis elegans
2.8.2.20 tyrosylprotein sulfotransferase
-
 Caenorhabditis elegans

General Information

EC Number General Information Comment Organism
2.8.2.20 malfunction knockdown of the enzyme blocks the cuticle localization of ROL-6 Caenorhabditis elegans
2.8.2.20 physiological function the enzyme regulates cuticle organization by promoting the transport of ROL-6 from the trans Golgi network to the cuticle. The sulfation of ROL-6 by TPST-1 is critical for its proper localization Caenorhabditis elegans