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Literature summary extracted from

  • Yoon, H.J.; Kang, K.Y.; Ahn, H.J.; Shim, S.M.; Ha, J.Y.; Lee, S.K.; Mikami, B.; Suh, S.W.
    X-ray crystallographic studies of HemK from Thermotoga maritima, an N5-glutamine methyltransferase (2003), Mol. Cells, 16, 266-269.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.297 overexpressed in Escherichia coli C41(DE3) Thermotoga maritima

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.297 crystallized in the presence of S-adenosylmethionine at 223°C using ammonium sulfate as the precipitant. X-ray diffraction data are collected to 2.5 A resolution from a native crystal. The crystal is orthorhombic, belonging to the space group I222 (or I2(1)2(1)2(1)), with unit-cell parameters of a = 104.24, b = 118.73, and c = 146.62 A. Two (or three) monomers of recombinant HemK are likely to be present in the crystallographic asymmetric unit Thermotoga maritima

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.297 S-adenosyl-L-methionine + [peptide chain release factor 1 or 2]-L-glutamine Thermotoga maritima
-
 S-adenosyl-L-homocysteine + [peptide chain release factor 1 or 2]-N5-methyl-L-glutamine
-
 ?
2.1.1.297 S-adenosyl-L-methionine + [peptide chain release factor 1 or 2]-L-glutamine Thermotoga maritima DSM 3109
-
 S-adenosyl-L-homocysteine + [peptide chain release factor 1 or 2]-N5-methyl-L-glutamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.297 Thermotoga maritima Q9WYV8
-
-
2.1.1.297 Thermotoga maritima DSM 3109 Q9WYV8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.297
-
 Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.297 S-adenosyl-L-methionine + [peptide chain release factor 1 or 2]-L-glutamine
-
 Thermotoga maritima S-adenosyl-L-homocysteine + [peptide chain release factor 1 or 2]-N5-methyl-L-glutamine
-
 ?
2.1.1.297 S-adenosyl-L-methionine + [peptide chain release factor 1 or 2]-L-glutamine
-
 Thermotoga maritima DSM 3109 S-adenosyl-L-homocysteine + [peptide chain release factor 1 or 2]-N5-methyl-L-glutamine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.297 HemK
-
 Thermotoga maritima
2.1.1.297 N5-glutamine MTase
-
 Thermotoga maritima
2.1.1.297 N5-glutamine S-adenosyl-L-methionine dependent methyltransferase
-
 Thermotoga maritima
2.1.1.297 PrmC
-
 Thermotoga maritima
2.1.1.297 TM0488 locus name Thermotoga maritima

General Information

EC Number General Information Comment Organism
2.1.1.297 physiological function the enzyme modifies the glutamine residue in the universally conserved glycylglycylglutamine (GGQ) motif of peptide chain release factor, resulting in almost complete loss of release activity Thermotoga maritima