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Literature summary extracted from

  • Qi, X.; Deng, W.; Wang, F.; Guo, Q.; Chen, H.; Wang, L.; He, X.; Huang, R.
    Molecular cloning, co-expression, and characterization of glycerol dehydratase and 1,3-propanediol dehydrogenase from Citrobacter freundii (2013), Mol. Biotechnol., 54, 469-474.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.202 expression in Escherichia coli Citrobacter freundii
4.2.1.30 expressed in Escherichia coli JM109 cells Citrobacter freundii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.202 1.28
-
 Propane-1,3-diol pH 10.5, 25°C Citrobacter freundii
1.1.1.202 10.05
-
 3-hydroxypropanal pH 8.0, 37°C Citrobacter freundii
4.2.1.30 0.25
-
 Propane-1,2-diol at pH 8.5 and 45°C Citrobacter freundii
4.2.1.30 0.59
-
 glycerol at pH 8.5 and 45°C Citrobacter freundii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.202 42000
-
 x * 42000, SDS-PAGE Citrobacter freundii
4.2.1.30 16000
-
 x * 61000 + x * 22000 + x * 16000, SDS-PAGE Citrobacter freundii
4.2.1.30 22000
-
 x * 61000 + x * 22000 + x * 16000, SDS-PAGE Citrobacter freundii
4.2.1.30 61000
-
 x * 61000 + x * 22000 + x * 16000, SDS-PAGE Citrobacter freundii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.30 glycerol Citrobacter freundii best substrate 3-hydroxypropanal + H2O
-
 ?
4.2.1.30 glycerol Citrobacter freundii AS1.1732 best substrate 3-hydroxypropanal + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.202 Citrobacter freundii P45513
-
-
4.2.1.30 Citrobacter freundii A8CH84 and A8CH91 and A8CH95 large, middle and small subunit
-
4.2.1.30 Citrobacter freundii AS1.1732 A8CH84 and A8CH91 and A8CH95 large, middle and small subunit
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.30 Ni-NTA agarose column chromatography and Sephacryl S-300 gel filtration Citrobacter freundii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.202 49.5
-
 reduction of 3-hydroxypropanal, pH 8.0, 37°C Citrobacter freundii
1.1.1.202 77.9
-
 oxidation of propane-1,3-diol, pH 10.5, 25°C Citrobacter freundii
4.2.1.30 21.2
-
 crude extract, at pH 8.5 and 45°C Citrobacter freundii
4.2.1.30 91.9
-
 after 4.3fold purification, at pH 8.5 and 45°C Citrobacter freundii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.202 3-hydroxypropanal + NADH + H+
-
 Citrobacter freundii propane-1,3-diol + NAD+
-
 r
1.1.1.202 propane-1,3-diol + NAD+
-
 Citrobacter freundii 3-hydroxypropanal + NADH + H+
-
 r
4.2.1.30 glycerol best substrate Citrobacter freundii 3-hydroxypropanal + H2O
-
 ?
4.2.1.30 glycerol best substrate Citrobacter freundii AS1.1732 3-hydroxypropanal + H2O
-
 ?
4.2.1.30 propane-1,2-diol
-
 Citrobacter freundii ?
-
 ?
4.2.1.30 propane-1,2-diol
-
 Citrobacter freundii AS1.1732 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.202 ? x * 42000, SDS-PAGE Citrobacter freundii
4.2.1.30 ? x * 61000 + x * 22000 + x * 16000, SDS-PAGE Citrobacter freundii

Synonyms

EC Number Synonyms Comment Organism
1.1.1.202 DhaT
-
 Citrobacter freundii
1.1.1.202 PDOR
-
 Citrobacter freundii
4.2.1.30 DhaB large subunit Citrobacter freundii
4.2.1.30 DhaC middle subunit Citrobacter freundii
4.2.1.30 DhaE small subunit Citrobacter freundii
4.2.1.30 GDHt
-
 Citrobacter freundii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.202 25
-
 oxidation of alcohol Citrobacter freundii
1.1.1.202 37
-
 reduction of aldehyde Citrobacter freundii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.30 45
-
 activity is lost dramatically at temperatures above 45°C Citrobacter freundii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.202 8
-
 reduction of aldehyde Citrobacter freundii
1.1.1.202 10.5
-
 oxidation of alcohol Citrobacter freundii
4.2.1.30 8.5
-
-
 Citrobacter freundii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.202 7 8.5 more than 80% of maximum activity for reduction of aldehyde Citrobacter freundii
1.1.1.202 7
-
 less than 10% of maximum activity for oxidation reaction Citrobacter freundii
4.2.1.30 7 9.5 the relative enzyme activity at pH values ranging from 7.0 to 9.5 is over 80%. Activity drops dramatically when the pH value is higher than 9.5 Citrobacter freundii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.202 NAD+
-
 Citrobacter freundii
1.1.1.202 NADH
-
 Citrobacter freundii