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Literature summary extracted from

  • Lin, Y.; Zhang, Z.; Tian, Y.; Zhao, W.; Zhu, B.; Xu, Z.; Peng, R.; Yao, Q.
    Purification and characterization of a novel laccase from Coprinus cinereus and decolorization of different chemically dyes (2013), Mol. Biol. Rep., 40, 1487-1494.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.10.3.2 gene CcLCC5I, expresssion in Pichia pastoris GS115 Coprinopsis cinerea

General Stability

EC Number General Stability Organism
1.10.3.2 the activity of CcLCC5I is stable after incubation for 16 h at 4°C in 0.2 M citrate-phosphate buffer at weakly alkaline conditions, the minimal effect on enzyme activity is at pH 8.6 Coprinopsis cinerea

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 Al3+ activating at 1 mM, inhibitory at 10 mM Coprinopsis cinerea
1.10.3.2 Ba2+ activating at 1 mM, inhibitory at 10 mM Coprinopsis cinerea
1.10.3.2 Ca2+ activating at 1 mM, inhibitory at 10 mM Coprinopsis cinerea
1.10.3.2 Cr3+ activating at 1 mM, inhibitory at 10 mM Coprinopsis cinerea
1.10.3.2 dithiothreitol
-
 Coprinopsis cinerea
1.10.3.2 Fe2+ activating at 1 mM, inhibitory at 10 mM Coprinopsis cinerea
1.10.3.2 Fe3+ activating at 1 mM, inhibitory at 10 mM Coprinopsis cinerea
1.10.3.2 K+ activating at 1 mM, inhibitory at 10 mM Coprinopsis cinerea
1.10.3.2 L-cysteine
-
 Coprinopsis cinerea
1.10.3.2 Li+ activating at 1 mM, inhibitory at 10 mM Coprinopsis cinerea
1.10.3.2 Mg2+ activating at 1 mM, inhibitory at 10 mM Coprinopsis cinerea
1.10.3.2 Mn2+ activating at 1 mM, inhibitory at 10 mM Coprinopsis cinerea
1.10.3.2 additional information except for copper ions, most metal ions inhibit the laccase activity at a high concentration of about 10 mM, EDTA has no inhibitory effect on the laccase activity Coprinopsis cinerea
1.10.3.2 Na+ activating at 1 mM, inhibitory at 10 mM Coprinopsis cinerea
1.10.3.2 NaN3
-
 Coprinopsis cinerea
1.10.3.2 sodium dodecylsulfate
-
 Coprinopsis cinerea
1.10.3.2 sodium sulfite
-
 Coprinopsis cinerea
1.10.3.2 sodium thiosulfate
-
 Coprinopsis cinerea
1.10.3.2 Zn2+ activating at 1 mM, inhibitory at 10 mM Coprinopsis cinerea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.086
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 3.0, 30°C Coprinopsis cinerea
1.10.3.2 17.6
-
 guaiacol pH 3.0, 30°C Coprinopsis cinerea

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 additional information except for copper ions, most metal ions inhibit the laccase activity at a high concentration of about 10 mM, EDTA has no inhibitory effect on the laccase activity Coprinopsis cinerea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 56800
-
 x * 56800, SDS-PAGE Coprinopsis cinerea

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Coprinopsis cinerea A8N3P5 gene CcLCC5I
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Coprinopsis cinerea ?
-
 ?
1.10.3.2 guaiacol + O2
-
 Coprinopsis cinerea ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 ? x * 56800, SDS-PAGE Coprinopsis cinerea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 55
-
 with substrate 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) Coprinopsis cinerea

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.10.3.2 30 70 optimum temperature of CcLCC5I is 55°C, the activity of laccase is high from 30 to 50°C, when the temperature is over 55°C, the activity declines more, when the temperature reaches 70°C, the relative activity of Lcc is only 23%, profile, overview Coprinopsis cinerea

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 60
-
 recombinant enzyme, stable up to Coprinopsis cinerea

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.10.3.2 0.211
-
 guaiacol pH 3.0, 30°C Coprinopsis cinerea
1.10.3.2 4.22
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 3.0, 30°C Coprinopsis cinerea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 3
-
 with substrate 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) Coprinopsis cinerea

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.10.3.2 2 7 activity range, profile, overview Coprinopsis cinerea

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.10.3.2 0.012
-
 guaiacol pH 3.0, 30°C Coprinopsis cinerea
1.10.3.2 49.1
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 3.0, 30°C Coprinopsis cinerea