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Literature summary extracted from

  • Wang, W.; Gao, J.; Wang, J.; Liu, C.; Meng, Y.
    Cloning, expression and enzymatic properties analysis of dihydrofolate reductase gene from the silkworm, Bombyx mori (2012), Mol. Biol. Rep., 39, 10285-10291.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.3 expressed in Escherichia coli BL21(DE3) cells Bombyx mori

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.3 0.03825
-
 7,8-dihydrofolate at pH 7.0 and 22°C Bombyx mori

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.3 21000
-
 x * 21000, calculated from amino acid sequence Bombyx mori
1.5.1.3 25000
-
 x * 25000, SDS-PAGE Bombyx mori

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.3 7,8-dihydrofolate + NADPH + H+ Bombyx mori
-
 5,6,7,8-tetrahydrofolate + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.3 Bombyx mori J7GQW2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.3 Ni-NTA column chromatography Bombyx mori

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.3 brain
-
 Bombyx mori
-
1.5.1.3 corpus allatum
-
 Bombyx mori
-
1.5.1.3 fat body
-
 Bombyx mori
-
1.5.1.3 malpighian tubule
-
 Bombyx mori
-
1.5.1.3 midgut
-
 Bombyx mori
-
1.5.1.3 silk gland
-
 Bombyx mori
-
1.5.1.3 testis
-
 Bombyx mori
-
1.5.1.3 trachea
-
 Bombyx mori
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.3 7,8-dihydrofolate + NADPH + H+
-
 Bombyx mori 5,6,7,8-tetrahydrofolate + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.3 ? x * 25000, SDS-PAGE Bombyx mori
1.5.1.3 ? x * 21000, calculated from amino acid sequence Bombyx mori

Synonyms

EC Number Synonyms Comment Organism
1.5.1.3 DHFR
-
 Bombyx mori

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.3 20 25
-
 Bombyx mori

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.5.1.3 30 60 when temperature is higher than 30°C, the enzyme activity is decreased rapidly but the comparatively lower velocity is maintained till 60°C Bombyx mori

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.3 NADPH
-
 Bombyx mori