BRENDA - Enzyme Database

The sterol-C7 desaturase from the ciliate Tetrahymena thermophila is a Rieske oxygenase, which is highly conserved in animals

Najle, S.R.; Nusblat, A.D.; Nudel, C.B.; Uttaro, A.D.; Mol. Biol. Evol. 30, 1630-1643 (2013)

Data extracted from this reference:

Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.3.1.21
membrane
-
Tetrahymena thermophila
16020
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.19.21
5alpha-cholest-22-en-3beta-ol + O2 + NADH + H+
Tetrahymena thermophila
-
5alpha-cholesta-7,22-dien-3beta-ol + NAD+ + H2O
-
-
?
1.14.19.21
5alpha-cholest-22-en-3beta-ol + O2 + NADPH + H+
Tetrahymena thermophila
-
5alpha-cholesta-7,22-dien-3beta-ol + NADP+ + H2O
-
-
?
1.14.19.21
5alpha-cholestan-3beta-ol + O2 + NADH + H+
Tetrahymena thermophila
-
5alpha-cholest-7-en-3beta-ol + NAD+ + H2O
-
-
?
1.14.19.21
5alpha-cholestan-3beta-ol + O2 + NADPH + H+
Tetrahymena thermophila
-
5alpha-cholest-7-en-3beta-ol + NADP+ + H2O
-
-
?
1.14.19.21
cholesta-5,22-dien-3beta-ol + O2 + NADH + H+
Tetrahymena thermophila
-
cholesta-5,7,22-trien-3beta-ol + NAD+ + H2O
-
-
?
1.14.19.21
cholesta-5,22-dien-3beta-ol + O2 + NADPH + H+
Tetrahymena thermophila
-
cholesta-5,7,22-trien-3beta-ol + NADP+ + H2O
-
-
?
1.14.19.21
cholesterol + O2 + NADH + H+
Tetrahymena thermophila
-
cholesta-5,7-dien-3beta-ol + NAD+ + H2O
-
-
?
1.14.19.21
cholesterol + O2 + NADPH + H+
Tetrahymena thermophila
-
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.3.1.21
Tetrahymena thermophila
I7ML19
-
-
1.14.19.21
Tetrahymena thermophila
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.3.1.21
cholesterol + NADP+
-
725923
Tetrahymena thermophila
cholesta-5,7-dien-3-beta-ol + NADPH + H+
-
-
-
?
1.14.19.21
5alpha-cholest-22-en-3beta-ol + O2 + NADH + H+
-
725923
Tetrahymena thermophila
5alpha-cholesta-7,22-dien-3beta-ol + NAD+ + H2O
-
-
-
?
1.14.19.21
5alpha-cholest-22-en-3beta-ol + O2 + NADPH + H+
-
725923
Tetrahymena thermophila
5alpha-cholesta-7,22-dien-3beta-ol + NADP+ + H2O
-
-
-
?
1.14.19.21
5alpha-cholestan-3beta-ol + O2 + NADH + H+
-
725923
Tetrahymena thermophila
5alpha-cholest-7-en-3beta-ol + NAD+ + H2O
-
-
-
?
1.14.19.21
5alpha-cholestan-3beta-ol + O2 + NADPH + H+
-
725923
Tetrahymena thermophila
5alpha-cholest-7-en-3beta-ol + NADP+ + H2O
-
-
-
?
1.14.19.21
cholesta-5,22-dien-3beta-ol + O2 + NADH + H+
-
725923
Tetrahymena thermophila
cholesta-5,7,22-trien-3beta-ol + NAD+ + H2O
-
-
-
?
1.14.19.21
cholesta-5,22-dien-3beta-ol + O2 + NADPH + H+
-
725923
Tetrahymena thermophila
cholesta-5,7,22-trien-3beta-ol + NADP+ + H2O
-
-
-
?
1.14.19.21
cholestanol + O2 + NADPH + H+
-
725923
Tetrahymena thermophila
cholesta-5,7,22-trien-3beta-ol + NADP+ + H2O
main product
-
-
?
1.14.19.21
cholesterol + O2 + NADH + H+
-
725923
Tetrahymena thermophila
cholesta-5,7-dien-3beta-ol + NAD+ + H2O
-
-
-
?
1.14.19.21
cholesterol + O2 + NADPH + H+
-
725923
Tetrahymena thermophila
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
-
?
1.14.19.21
stigmastanol + O2 + NADPH + H+
-
725923
Tetrahymena thermophila
5alpha-24-ethylcholest-7-en-3beta-ol + NADP+ + H2O
main product
-
-
?
1.14.19.21
stigmasterol + O2 + NADPH + H+
-
725923
Tetrahymena thermophila
(3beta,22E)-stigmasta-5,7,22-trien-3-ol + NADP+ + H2O
main product
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.19.21
NADH
-
Tetrahymena thermophila
1.14.19.21
NADPH
-
Tetrahymena thermophila
1.14.19.21
[2Fe-2S]-center
-
Tetrahymena thermophila
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.19.21
NADH
-
Tetrahymena thermophila
1.14.19.21
NADPH
-
Tetrahymena thermophila
1.14.19.21
[2Fe-2S]-center
-
Tetrahymena thermophila
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.3.1.21
membrane
-
Tetrahymena thermophila
16020
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.19.21
5alpha-cholest-22-en-3beta-ol + O2 + NADH + H+
Tetrahymena thermophila
-
5alpha-cholesta-7,22-dien-3beta-ol + NAD+ + H2O
-
-
?
1.14.19.21
5alpha-cholest-22-en-3beta-ol + O2 + NADPH + H+
Tetrahymena thermophila
-
5alpha-cholesta-7,22-dien-3beta-ol + NADP+ + H2O
-
-
?
1.14.19.21
5alpha-cholestan-3beta-ol + O2 + NADH + H+
Tetrahymena thermophila
-
5alpha-cholest-7-en-3beta-ol + NAD+ + H2O
-
-
?
1.14.19.21
5alpha-cholestan-3beta-ol + O2 + NADPH + H+
Tetrahymena thermophila
-
5alpha-cholest-7-en-3beta-ol + NADP+ + H2O
-
-
?
1.14.19.21
cholesta-5,22-dien-3beta-ol + O2 + NADH + H+
Tetrahymena thermophila
-
cholesta-5,7,22-trien-3beta-ol + NAD+ + H2O
-
-
?
1.14.19.21
cholesta-5,22-dien-3beta-ol + O2 + NADPH + H+
Tetrahymena thermophila
-
cholesta-5,7,22-trien-3beta-ol + NADP+ + H2O
-
-
?
1.14.19.21
cholesterol + O2 + NADH + H+
Tetrahymena thermophila
-
cholesta-5,7-dien-3beta-ol + NAD+ + H2O
-
-
?
1.14.19.21
cholesterol + O2 + NADPH + H+
Tetrahymena thermophila
-
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.3.1.21
cholesterol + NADP+
-
725923
Tetrahymena thermophila
cholesta-5,7-dien-3-beta-ol + NADPH + H+
-
-
-
?
1.14.19.21
5alpha-cholest-22-en-3beta-ol + O2 + NADH + H+
-
725923
Tetrahymena thermophila
5alpha-cholesta-7,22-dien-3beta-ol + NAD+ + H2O
-
-
-
?
1.14.19.21
5alpha-cholest-22-en-3beta-ol + O2 + NADPH + H+
-
725923
Tetrahymena thermophila
5alpha-cholesta-7,22-dien-3beta-ol + NADP+ + H2O
-
-
-
?
1.14.19.21
5alpha-cholestan-3beta-ol + O2 + NADH + H+
-
725923
Tetrahymena thermophila
5alpha-cholest-7-en-3beta-ol + NAD+ + H2O
-
-
-
?
1.14.19.21
5alpha-cholestan-3beta-ol + O2 + NADPH + H+
-
725923
Tetrahymena thermophila
5alpha-cholest-7-en-3beta-ol + NADP+ + H2O
-
-
-
?
1.14.19.21
cholesta-5,22-dien-3beta-ol + O2 + NADH + H+
-
725923
Tetrahymena thermophila
cholesta-5,7,22-trien-3beta-ol + NAD+ + H2O
-
-
-
?
1.14.19.21
cholesta-5,22-dien-3beta-ol + O2 + NADPH + H+
-
725923
Tetrahymena thermophila
cholesta-5,7,22-trien-3beta-ol + NADP+ + H2O
-
-
-
?
1.14.19.21
cholestanol + O2 + NADPH + H+
-
725923
Tetrahymena thermophila
cholesta-5,7,22-trien-3beta-ol + NADP+ + H2O
main product
-
-
?
1.14.19.21
cholesterol + O2 + NADH + H+
-
725923
Tetrahymena thermophila
cholesta-5,7-dien-3beta-ol + NAD+ + H2O
-
-
-
?
1.14.19.21
cholesterol + O2 + NADPH + H+
-
725923
Tetrahymena thermophila
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
-
?
1.14.19.21
stigmastanol + O2 + NADPH + H+
-
725923
Tetrahymena thermophila
5alpha-24-ethylcholest-7-en-3beta-ol + NADP+ + H2O
main product
-
-
?
1.14.19.21
stigmasterol + O2 + NADPH + H+
-
725923
Tetrahymena thermophila
(3beta,22E)-stigmasta-5,7,22-trien-3-ol + NADP+ + H2O
main product
-
-
?
General Information
EC Number
General Information
Commentary
Organism
1.3.1.21
physiological function
a knock out strain shows no differences in growth rates nor cell shape, neither in the presence nor in the absence of added sterols.Sterols found in wild-type are 83.44% cholesta-5,7,22-trien-3beta-ol as the major derivative, besides cholesterol itself, 0.55% cholesta-5,7-dien-3beta-ol and 3.84% cholesta-5,22-dien-3beta-ol. In contrast, in the KODES7 cell line, the only conversion product recovered is 92.76% 22-dehydrocholesterol
Tetrahymena thermophila
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.3.1.21
physiological function
a knock out strain shows no differences in growth rates nor cell shape, neither in the presence nor in the absence of added sterols.Sterols found in wild-type are 83.44% cholesta-5,7,22-trien-3beta-ol as the major derivative, besides cholesterol itself, 0.55% cholesta-5,7-dien-3beta-ol and 3.84% cholesta-5,22-dien-3beta-ol. In contrast, in the KODES7 cell line, the only conversion product recovered is 92.76% 22-dehydrocholesterol
Tetrahymena thermophila