Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hortua Triana, M.A.; Huynh, M.H.; Garavito, M.F.; Fox, B.A.; Bzik, D.J.; Carruthers, V.B.; Loeffler, M.; Zimmermann, B.H.
    Biochemical and molecular characterization of the pyrimidine biosynthetic enzyme dihydroorotate dehydrogenase from Toxoplasma gondii (2012), Mol. Biochem. Parasitol., 184, 71-81.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.5.2 2-cyano-3-hydroxy-N-(2',3,3'-trichlorobiphenyl-4-yl)but-2-enamide
-
 Toxoplasma gondii
1.3.5.2 2-hydroxyethylidene-cyano acetic acid 4-trifluoromethyl anilide A177-1726, active derivative of human inhibitor leflunomide Toxoplasma gondii
1.3.5.2 additional information not inhibitory: brequinar, atovaquone, N-(3,5-difluoro-4-(trifluoromethyl)phenyl)-5-methyl-[1,2,4]triazolo[1,5-a]pyrimidin-7-amine Toxoplasma gondii
1.3.5.2 N-(3-chloro-2'-methoxybiphenyl-4-yl)-2-cyano-3-hydroxybut-2-enamide
-
 Toxoplasma gondii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.5.2 0.027
-
 ubiquinone-50 pH 8.0, 30°C Toxoplasma gondii
1.3.5.2 0.028
-
 1,4-Naphthoquinone pH 8.0, 30°C Toxoplasma gondii
1.3.5.2 0.029
-
 decylubiquinone pH 8.0, 30°C Toxoplasma gondii
1.3.5.2 0.051
-
 ubiquinone-30 pH 8.0, 30°C Toxoplasma gondii
1.3.5.2 0.06
-
 (S)-dihydroorotate pH 8.0, 30°C Toxoplasma gondii
1.3.5.2 0.099
-
 2,3-dimethoxy-5-methyl-1,4-benzoquinone pH 8.0, 30°C Toxoplasma gondii
1.3.5.2 0.132
-
 2,5-dimethyl-p-benzoquinone pH 8.0, 30°C Toxoplasma gondii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.5.2 mitochondrion
-
 Toxoplasma gondii 5739
-

Organism

EC Number Organism UniProt Comment Textmining
1.3.5.2 Toxoplasma gondii
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.5.2 84
-
 pH 8.0, 30°C Toxoplasma gondii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.5.2 (S)-dihydroorotate + 1,4-naphthoquinone
-
 Toxoplasma gondii orotate + 1,4-naphthoquinol
-
 ?
1.3.5.2 (S)-dihydroorotate + 2,3-dimethoxy-5-methyl-1,4-benzoquinone
-
 Toxoplasma gondii orotate + 2,3-dimethoxy-5-methyl-1,4-benzoquinol
-
 ?
1.3.5.2 (S)-dihydroorotate + 2,5-dimethyl-p-benzoquinone
-
 Toxoplasma gondii orotate + 2,5-dimethyl-p-benzoquinol
-
 ?
1.3.5.2 (S)-dihydroorotate + decylubiquinone
-
 Toxoplasma gondii orotate + decylubiquinol
-
 ?
1.3.5.2 (S)-dihydroorotate + ubiquinone-30
-
 Toxoplasma gondii orotate + ubiquinol-30
-
 ?
1.3.5.2 (S)-dihydroorotate + ubiquinone-50
-
 Toxoplasma gondii orotate + ubiquinol-50
-
 ?
1.3.5.2 additional information fumarate is a poor electron acceptor Toxoplasma gondii ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.5.2 21
-
 ubiquinone-30 pH 8.0, 30°C Toxoplasma gondii
1.3.5.2 29
-
 1,4-Naphthoquinone pH 8.0, 30°C Toxoplasma gondii
1.3.5.2 32
-
 2,3-dimethoxy-5-methyl-1,4-benzoquinone pH 8.0, 30°C Toxoplasma gondii
1.3.5.2 44
-
 2,5-dimethyl-p-benzoquinone pH 8.0, 30°C Toxoplasma gondii
1.3.5.2 61
-
 ubiquinone-50 pH 8.0, 30°C Toxoplasma gondii
1.3.5.2 82
-
 (S)-dihydroorotate pH 8.0, 30°C Toxoplasma gondii
1.3.5.2 89
-
 decylubiquinone pH 8.0, 30°C Toxoplasma gondii

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.3.5.2 0.06
-
 pH 8.0, 30°C Toxoplasma gondii N-(3-chloro-2'-methoxybiphenyl-4-yl)-2-cyano-3-hydroxybut-2-enamide
1.3.5.2 0.091
-
 pH 8.0, 30°C Toxoplasma gondii 2-hydroxyethylidene-cyano acetic acid 4-trifluoromethyl anilide
1.3.5.2 0.096
-
 pH 8.0, 30°C Toxoplasma gondii 2-cyano-3-hydroxy-N-(2',3,3'-trichlorobiphenyl-4-yl)but-2-enamide

General Information

EC Number General Information Comment Organism
1.3.5.2 physiological function the gene appears to be essential, because while gene tagging is successful at the TgDHOD gene locus, attempts to delete the TgDHOD gene were not successful in the KU80 background Toxoplasma gondii