BRENDA - Enzyme Database

Reduction of quinones and phenoxy radicals by extracellular glucose dehydrogenase from Glomerella cingulata suggests a role in plant pathogenicity

Sygmund, C.; Klausberger, M.; Felice, A.K.; Ludwig, R.; Microbiology 157, 3203-3212 (2011)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.1.5.9
DNA and amino acid sequence determination and analysis, phylogenetic analysis
Colletotrichum gloeosporioides
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.5.9
extracellular
-
Colletotrichum gloeosporioides
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.5.9
68000
-
1 * 68000, deglycosylated enzyme, SDS-PAGE
Colletotrichum gloeosporioides
1.1.5.9
95000
135000
glycoprotein, native PAGE
Colletotrichum gloeosporioides
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.5.9
D-glucose + a quinone
Colletotrichum gloeosporioides
-
D-glucono-1,5-lactone + a quinol
-
-
?
1.1.5.9
D-glucose + a quinone
Colletotrichum gloeosporioides DSM 62728
-
D-glucono-1,5-lactone + a quinol
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.5.9
Colletotrichum gloeosporioides
-
anamorph Colletotrichum gloeosporoides
-
1.1.5.9
Colletotrichum gloeosporioides DSM 62728
-
anamorph Colletotrichum gloeosporoides
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
1.1.5.9
glycoprotein
four potential N-glycosylation sites at N55, N233, N255 and N339
Colletotrichum gloeosporioides
Purification (Commentary)
EC Number
Commentary
Organism
1.1.5.9
native extracellular enzyme 23.7fold from culture supernatant by anion exchange and hydrophobic interaction chromatography, ammonium sulfate fractionation, and ultrafiltration
Colletotrichum gloeosporioides
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.5.9
565
-
purified enzyme with ferrocenium hexafluorophosphate, pH 5.5, 30°C
Colletotrichum gloeosporioides
1.1.5.9
840
-
purified enzyme with 2,6-dichlorophenol indophenol , pH 5.5, 30°C
Colletotrichum gloeosporioides
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.5.9
D-glucose + 1,4-benzoquinone
-
725906
Colletotrichum gloeosporioides
D-glucono-1,5-lactone + 1,4-benzoquinol
-
-
-
?
1.1.5.9
D-glucose + 1,4-benzoquinone
-
725906
Colletotrichum gloeosporioides DSM 62728
D-glucono-1,5-lactone + 1,4-benzoquinol
-
-
-
?
1.1.5.9
D-glucose + a quinone
-
725906
Colletotrichum gloeosporioides
D-glucono-1,5-lactone + a quinol
-
-
-
?
1.1.5.9
D-glucose + a quinone
-
725906
Colletotrichum gloeosporioides DSM 62728
D-glucono-1,5-lactone + a quinol
-
-
-
?
1.1.5.9
D-glucose + ferricenium hexafluorophosphate
-
725906
Colletotrichum gloeosporioides
D-glucono-1,5-lactone + ferrocenium hexafluorophosphate
-
-
-
?
1.1.5.9
D-glucose + ferricenium hexafluorophosphate
-
725906
Colletotrichum gloeosporioides DSM 62728
D-glucono-1,5-lactone + ferrocenium hexafluorophosphate
-
-
-
?
1.1.5.9
D-glucose + ferricyanide
-
725906
Colletotrichum gloeosporioides
D-glucono-1,5-lactone + ferrocyanide
-
-
-
?
1.1.5.9
D-glucose + ferricyanide
-
725906
Colletotrichum gloeosporioides DSM 62728
D-glucono-1,5-lactone + ferrocyanide
-
-
-
?
1.1.5.9
D-glucose + oxidized 2,6-dichlorophenol indophenol
-
725906
Colletotrichum gloeosporioides
D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
-
-
?
1.1.5.9
additional information
suitable electron acceptors are quinones, phenoxy radicals, 2,6-dichloroindophenol, ferricyanide and ferrocenium hexafluorophosphate. Reduction of quinones and phenoxy radicals by extracellular enzyme, overview
725906
Colletotrichum gloeosporioides
?
-
-
-
-
1.1.5.9
additional information
suitable electron acceptors are quinones, phenoxy radicals, 2,6-dichloroindophenol, ferricyanide and ferrocenium hexafluorophosphate. Reduction of quinones and phenoxy radicals by extracellular enzyme, overview
725906
Colletotrichum gloeosporioides DSM 62728
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
1.1.5.9
monomer
1 * 68000, deglycosylated enzyme, SDS-PAGE
Colletotrichum gloeosporioides
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.5.9
30
-
assay at
Colletotrichum gloeosporioides
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.5.9
5.5
-
assay at with 2,6-dichlorophenol indophenol or ferrocenium hexafluorophosphate
Colletotrichum gloeosporioides
1.1.5.9
7.5
-
assay at with 1,4-benzoquinone
Colletotrichum gloeosporioides
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.5.9
FAD
dependent on, non-covalently bound
Colletotrichum gloeosporioides
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
1.1.5.9
Colletotrichum gloeosporioides
isoelectric focusing
-
5.6
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.5.9
DNA and amino acid sequence determination and analysis, phylogenetic analysis
Colletotrichum gloeosporioides
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.5.9
FAD
dependent on, non-covalently bound
Colletotrichum gloeosporioides
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.5.9
extracellular
-
Colletotrichum gloeosporioides
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.5.9
68000
-
1 * 68000, deglycosylated enzyme, SDS-PAGE
Colletotrichum gloeosporioides
1.1.5.9
95000
135000
glycoprotein, native PAGE
Colletotrichum gloeosporioides
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.5.9
D-glucose + a quinone
Colletotrichum gloeosporioides
-
D-glucono-1,5-lactone + a quinol
-
-
?
1.1.5.9
D-glucose + a quinone
Colletotrichum gloeosporioides DSM 62728
-
D-glucono-1,5-lactone + a quinol
-
-
?
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
1.1.5.9
glycoprotein
four potential N-glycosylation sites at N55, N233, N255 and N339
Colletotrichum gloeosporioides
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.5.9
native extracellular enzyme 23.7fold from culture supernatant by anion exchange and hydrophobic interaction chromatography, ammonium sulfate fractionation, and ultrafiltration
Colletotrichum gloeosporioides
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.5.9
565
-
purified enzyme with ferrocenium hexafluorophosphate, pH 5.5, 30°C
Colletotrichum gloeosporioides
1.1.5.9
840
-
purified enzyme with 2,6-dichlorophenol indophenol , pH 5.5, 30°C
Colletotrichum gloeosporioides
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.5.9
D-glucose + 1,4-benzoquinone
-
725906
Colletotrichum gloeosporioides
D-glucono-1,5-lactone + 1,4-benzoquinol
-
-
-
?
1.1.5.9
D-glucose + 1,4-benzoquinone
-
725906
Colletotrichum gloeosporioides DSM 62728
D-glucono-1,5-lactone + 1,4-benzoquinol
-
-
-
?
1.1.5.9
D-glucose + a quinone
-
725906
Colletotrichum gloeosporioides
D-glucono-1,5-lactone + a quinol
-
-
-
?
1.1.5.9
D-glucose + a quinone
-
725906
Colletotrichum gloeosporioides DSM 62728
D-glucono-1,5-lactone + a quinol
-
-
-
?
1.1.5.9
D-glucose + ferricenium hexafluorophosphate
-
725906
Colletotrichum gloeosporioides
D-glucono-1,5-lactone + ferrocenium hexafluorophosphate
-
-
-
?
1.1.5.9
D-glucose + ferricenium hexafluorophosphate
-
725906
Colletotrichum gloeosporioides DSM 62728
D-glucono-1,5-lactone + ferrocenium hexafluorophosphate
-
-
-
?
1.1.5.9
D-glucose + ferricyanide
-
725906
Colletotrichum gloeosporioides
D-glucono-1,5-lactone + ferrocyanide
-
-
-
?
1.1.5.9
D-glucose + ferricyanide
-
725906
Colletotrichum gloeosporioides DSM 62728
D-glucono-1,5-lactone + ferrocyanide
-
-
-
?
1.1.5.9
D-glucose + oxidized 2,6-dichlorophenol indophenol
-
725906
Colletotrichum gloeosporioides
D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
-
-
?
1.1.5.9
additional information
suitable electron acceptors are quinones, phenoxy radicals, 2,6-dichloroindophenol, ferricyanide and ferrocenium hexafluorophosphate. Reduction of quinones and phenoxy radicals by extracellular enzyme, overview
725906
Colletotrichum gloeosporioides
?
-
-
-
-
1.1.5.9
additional information
suitable electron acceptors are quinones, phenoxy radicals, 2,6-dichloroindophenol, ferricyanide and ferrocenium hexafluorophosphate. Reduction of quinones and phenoxy radicals by extracellular enzyme, overview
725906
Colletotrichum gloeosporioides DSM 62728
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.5.9
monomer
1 * 68000, deglycosylated enzyme, SDS-PAGE
Colletotrichum gloeosporioides
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.5.9
30
-
assay at
Colletotrichum gloeosporioides
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.5.9
5.5
-
assay at with 2,6-dichlorophenol indophenol or ferrocenium hexafluorophosphate
Colletotrichum gloeosporioides
1.1.5.9
7.5
-
assay at with 1,4-benzoquinone
Colletotrichum gloeosporioides
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
1.1.5.9
Colletotrichum gloeosporioides
isoelectric focusing
-
5.6
General Information
EC Number
General Information
Commentary
Organism
1.1.5.9
evolution
the enzyme belongs to the to the GMC family of oxidoreductases, phylogenetic analysis
Colletotrichum gloeosporioides
1.1.5.9
physiological function
the enzyme from Glomerella cingulata might play a role in plant pathogenicity. The quinone and phenoxy radical reducing enzyme light act to neutralize the action of plant laccase, phenoloxidase or peroxidase activities, which are increased in infected plants to evade fungal attack
Colletotrichum gloeosporioides
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.1.5.9
evolution
the enzyme belongs to the to the GMC family of oxidoreductases, phylogenetic analysis
Colletotrichum gloeosporioides
1.1.5.9
physiological function
the enzyme from Glomerella cingulata might play a role in plant pathogenicity. The quinone and phenoxy radical reducing enzyme light act to neutralize the action of plant laccase, phenoloxidase or peroxidase activities, which are increased in infected plants to evade fungal attack
Colletotrichum gloeosporioides