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Literature summary extracted from

  • Hozuki, T.; Ohtsuka, T.; Arai, K.; Yoshimatsu, K.; Tanaka, S.; Fujiwara, T.
    Effect of salinity on hydroxylamine oxidation in a marine ammonia-oxidizing gammaproteobacterium, Nitrosococcus oceani strain NS58: molecular and catalytic properties of tetraheme cytochrome c-554 (2010), Microbes Environ., 25, 95-102.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.2.6 0.0009
-
 ferricyanide pH 7.8, temperature not specified in the publication Nitrosococcus oceani
1.7.2.6 0.0011
-
 ferricytochrome c-554 source of substrate Nitrosococcus oceani, pH 7.8, temperature not specified in the publication Nitrosococcus oceani

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.2.6 180000
-
 x * 180000, SDS-PAGE Nitrosococcus oceani

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.6 Nitrosococcus oceani M5DCM0
-
-
1.7.2.6 Nitrosococcus oceani NS58 M5DCM0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.2.6
-
 Nitrosococcus oceani

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.6 hydroxylamine + ferricytochrome c-554 + H2O
-
 Nitrosococcus oceani nitrite + ferrocytochrome c-554 + H+
-
 ?
1.7.2.6 hydroxylamine + ferricytochrome c-554 + H2O
-
 Nitrosococcus oceani NS58 nitrite + ferrocytochrome c-554 + H+
-
 ?
1.7.2.6 hydroxylamine + H2O + 2 ferricyanide
-
 Nitrosococcus oceani nitrite + 2 ferrocyanide + 5 H+
-
 ?
1.7.2.6 hydroxylamine + H2O + 2 ferricyanide
-
 Nitrosococcus oceani NS58 nitrite + 2 ferrocyanide + 5 H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.2.6 ? x * 180000, SDS-PAGE Nitrosococcus oceani
1.7.2.6 More the enzyme is composed of a homotrimer of subunits with molecular weight of 62000 Da covalently bound to each other. The bonds are mediated by prosthetic heme c molecules Nitrosococcus oceani

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7.2.6 3.4
-
 ferricytochrome c-554 source of substrate Nitrosococcus oceani, pH 7.8, temperature not specified in the publication Nitrosococcus oceani
1.7.2.6 111
-
 ferricyanide pH 7.8, temperature not specified in the publication Nitrosococcus oceani

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.2.6 cytochrome c purified enzyme shows typical cytochrome-like absorption spectra, but with a peculiar peak at 468 nm in the reduced state that is due to a P460 moiety corresponding to a modified heme c molecule Nitrosococcus oceani
1.7.2.6 heme protein contains at least 16 hemes c per molecule Nitrosococcus oceani