EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.99.18 | expressed in Saccharomyces cerevisiae strain BJ5465 | Thermothelomyces myriococcoides |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.99.18 | N700S | the kcat for oxygen turnover is increased in this mutant (4.5fold), but also substrate turnover. A 3fold increase of the kcat for cellobiose with alternative electron acceptors indicates that mutation N700S influences the oxidative- and reductive FAD half-reaction | Thermothelomyces myriococcoides |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.99.18 | 0.01147 | - |
2,6-dichlorophenol indophenol | recombinant wild type enzyme, in 100 mM McIlvaine buffer, pH 6.0, at 30°C | Thermothelomyces myriococcoides | |
1.1.99.18 | 0.022 | - |
1,4-benzoquinone | recombinant wild type enzyme, in 100 mM McIlvaine buffer, pH 6.0, at 30°C | Thermothelomyces myriococcoides | |
1.1.99.18 | 0.033 | - |
2,6-dichlorophenol indophenol | mutant enzyme N700S, in 100 mM McIlvaine buffer, pH 6.0, at 30°C | Thermothelomyces myriococcoides | |
1.1.99.18 | 0.037 | - |
O2 | recombinant wild type enzyme, in 100 mM McIlvaine buffer, pH 6.0, at 30°C | Thermothelomyces myriococcoides | |
1.1.99.18 | 0.056 | - |
1,4-benzoquinone | mutant enzyme 700S, in 100 mM McIlvaine buffer, pH 6.0, at 30°C | Thermothelomyces myriococcoides | |
1.1.99.18 | 0.073 | - |
O2 | mutant enzyme N700S, in 100 mM McIlvaine buffer, pH 6.0, at 30°C | Thermothelomyces myriococcoides |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.99.18 | cellobiose + 1,4-benzoquinone | Thermothelomyces myriococcoides | - |
cellobiono-1,5-lactone + reduced 1,4-benzoquinone | - |
? | |
1.1.99.18 | cellobiose + 2,6-dichlorophenol indophenol | Thermothelomyces myriococcoides | - |
cellobiono-1,5-lactone + reduced 2,6-dichlorophenol indophenol | - |
? | |
1.1.99.18 | cellobiose + O2 | Thermothelomyces myriococcoides | - |
cellobiono-1,5-lactone + H2O2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.99.18 | Thermothelomyces myriococcoides | A9XK88 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.99.18 | phenyl Sepharose column chromatography and Q-Source column chromatography | Thermothelomyces myriococcoides |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.99.18 | 2.2 | - |
culture supernatant, at pH 6.0 and 30°C | Thermothelomyces myriococcoides |
1.1.99.18 | 8 | - |
after 3.6fold purification, at pH 6.0 and 30°C | Thermothelomyces myriococcoides |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.99.18 | cellobiose + 1,4-benzoquinone | - |
Thermothelomyces myriococcoides | cellobiono-1,5-lactone + reduced 1,4-benzoquinone | - |
? | |
1.1.99.18 | cellobiose + 2,6-dichlorophenol indophenol | - |
Thermothelomyces myriococcoides | cellobiono-1,5-lactone + reduced 2,6-dichlorophenol indophenol | - |
? | |
1.1.99.18 | cellobiose + O2 | - |
Thermothelomyces myriococcoides | cellobiono-1,5-lactone + H2O2 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.99.18 | Cdh | - |
Thermothelomyces myriococcoides |
1.1.99.18 | cellobiose oxidase | - |
Thermothelomyces myriococcoides |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.99.18 | 0.042 | - |
O2 | recombinant wild type enzyme, in 100 mM McIlvaine buffer, pH 6.0, at 30°C | Thermothelomyces myriococcoides | |
1.1.99.18 | 0.19 | - |
O2 | mutant enzyme N700S, in 100 mM McIlvaine buffer, pH 6.0, at 30°C | Thermothelomyces myriococcoides | |
1.1.99.18 | 4.1 | - |
2,6-dichlorophenol indophenol | recombinant wild type enzyme, in 100 mM McIlvaine buffer, pH 6.0, at 30°C | Thermothelomyces myriococcoides | |
1.1.99.18 | 12.4 | - |
2,6-dichlorophenol indophenol | mutant enzyme N700S, in 100 mM McIlvaine buffer, pH 6.0, at 30°C | Thermothelomyces myriococcoides | |
1.1.99.18 | 13.4 | - |
1,4-benzoquinone | mutant enzyme 700S, in 100 mM McIlvaine buffer, pH 6.0, at 30°C | Thermothelomyces myriococcoides | |
1.1.99.18 | 43 | - |
1,4-benzoquinone | recombinant wild type enzyme, in 100 mM McIlvaine buffer, pH 6.0, at 30°C | Thermothelomyces myriococcoides |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.99.18 | FAD | - |
Thermothelomyces myriococcoides |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.99.18 | 1.1 | - |
O2 | recombinant wild type enzyme, in 100 mM McIlvaine buffer, pH 6.0, at 30°C | Thermothelomyces myriococcoides | |
1.1.99.18 | 2.6 | - |
O2 | mutant enzyme N700S, in 100 mM McIlvaine buffer, pH 6.0, at 30°C | Thermothelomyces myriococcoides | |
1.1.99.18 | 185 | - |
1,4-benzoquinone | recombinant wild type enzyme, in 100 mM McIlvaine buffer, pH 6.0, at 30°C | Thermothelomyces myriococcoides | |
1.1.99.18 | 240 | - |
1,4-benzoquinone | mutant enzyme 700S, in 100 mM McIlvaine buffer, pH 6.0, at 30°C | Thermothelomyces myriococcoides | |
1.1.99.18 | 360 | - |
2,6-dichlorophenol indophenol | recombinant wild type enzyme, in 100 mM McIlvaine buffer, pH 6.0, at 30°C | Thermothelomyces myriococcoides | |
1.1.99.18 | 375 | - |
2,6-dichlorophenol indophenol | mutant enzyme N700S, in 100 mM McIlvaine buffer, pH 6.0, at 30°C | Thermothelomyces myriococcoides |