EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.15.1 | G326A | site-directed mutagenesis in order to decrease the flexibility of the polypeptide at that point, spin state fractions with different substrates and compared to the wild-type enzyme. The mutant shows 40% reduced activity compared to the wild-type enzyme | Pseudomonas putida |
1.14.15.1 | additional information | generation of a G327 insertion mutant in order to determine whether the length of the helix played a role in the sensitivity of the K' helix to substrate, the insertion mutant fails to express | Pseudomonas putida |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.15.1 | Fe2+ | heme and cytochrome P450 containing enzyme | Pseudomonas putida |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.15.1 | (+)-camphor + reduced putidaredoxin + O2 | Pseudomonas putida | - |
(+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.15.1 | Pseudomonas putida | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.15.1 | cell culture | Pseudomonas putida strains can use (1R)-(+) camphor as sole carbon and energy source | Pseudomonas putida | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.15.1 | (+)-camphor + reduced putidaredoxin + O2 | - |
Pseudomonas putida | (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O | - |
? | |
1.14.15.1 | adamantenone + reduced putidaredoxin + O2 | - |
Pseudomonas putida | ? | - |
? | |
1.14.15.1 | norcamphor + reduced putidaredoxin + O2 | - |
Pseudomonas putida | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.15.1 | CYP101A1 | - |
Pseudomonas putida |
1.14.15.1 | cytochrome P450 cam | - |
Pseudomonas putida |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.15.1 | cytochrome P450 | - |
Pseudomonas putida | |
1.14.15.1 | heme | - |
Pseudomonas putida | |
1.14.15.1 | putidaredoxin | - |
Pseudomonas putida |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.15.1 | evolution | the enzyme belongs to the superfamily of cytochrome P450 monooxygenases | Pseudomonas putida |
1.14.15.1 | additional information | substrate recognition and selectivity, enzyme-substrate interactions, NMR structrue analysis, Analysis of 1H,15N-TROSY-HSQC spectra of CYP-(+)-camphor-CO, CYP-adamantenone-CO and CYP-norcamphor-CO. overview. Replacing the native substrate camphor with adamantanone or norcamphor causes perturbations in NMR-detected NH correlations assigned to the network,which includes portions of a beta-sheet and an adjacent helix that is remote from the active site | Pseudomonas putida |