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Literature summary extracted from

  • Toivari, M.; Nygard, Y.; Kumpula, E.P.; Vehkomaeki, M.L.; Bencina, M.; Valkonen, M.; Maaheimo, H.; Andberg, M.; Koivula, A.; Ruohonen, L.; Penttilae, M.; Wiebe, M.G.
    Metabolic engineering of Saccharomyces cerevisiae for bioconversion of D-xylose to D-xylonate (2012), Metab. Eng., 14, 427-436.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.175 synthesis expression of gene xylB in Saccharomyces cerevisiae results in production of 17 g D-xylonate/l at 0.23g/l/h from 23 g D-xylose/l. D-Xylonate accumulates intracellularly to 70 mg/g, xylitol to 18 mg/g. Cells expressing D-xylonolactone lactonase xylC from Caulobacter crescentus with xylB initially produce more extracellular D-xylonate than cells lacking xylC at both pH5.5 and pH3, and sustain higher production at pH3. Cell vitality and viability decreases during D-xylonate production at pH 3.0 Caulobacter vibrioides

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.175 expression in Saccharomyces cerevisiae Caulobacter vibrioides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.175 0.08
-
 D-xylose 25°C, pH 9.0 Caulobacter vibrioides
1.1.1.175 0.24
-
 D-xylose 25°C, pH 7.0 Caulobacter vibrioides
1.1.1.175 0.26
-
 NAD+ 25°C, pH 6.8 Caulobacter vibrioides
1.1.1.175 0.31
-
 NAD+ 25°C, pH 7.0 Caulobacter vibrioides
1.1.1.175 0.4
-
 D-xylose 25°C, pH 6.8 Caulobacter vibrioides
1.1.1.175 0.46
-
 NAD+ 25°C, pH 9.0 Caulobacter vibrioides
1.1.1.175 40
-
 L-arabinose 25°C, pH 9.0 Caulobacter vibrioides
1.1.1.175 100
-
 L-arabinose 25°C, pH 7.0 Caulobacter vibrioides
1.1.1.175 180
-
 L-arabinose 25°C, pH 6.8 Caulobacter vibrioides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.175 D-xylose + NAD+ Caulobacter vibrioides
-
 D-xylonolactone + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.175 Caulobacter vibrioides B8H1Z0 putative
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.175 D-xylose + NAD+
-
 Caulobacter vibrioides D-xylonolactone + NADH + H+
-
 ?
1.1.1.175 L-arabinose + NAD+
-
 Caulobacter vibrioides L-arabinono-1,4-lactone + NADH + H+
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.175 22.3
-
 L-arabinose 25°C, pH 6.8 Caulobacter vibrioides
1.1.1.175 22.7
-
 D-xylose 25°C, pH 9.0 Caulobacter vibrioides
1.1.1.175 24.2
-
 L-arabinose 25°C, pH 9.0 Caulobacter vibrioides
1.1.1.175 25.8
-
 NAD+ 25°C, pH 6.8 Caulobacter vibrioides
1.1.1.175 26.2
-
 D-xylose 25°C, pH 6.8 Caulobacter vibrioides
1.1.1.175 29.2
-
 L-arabinose 25°C, pH 7.0 Caulobacter vibrioides
1.1.1.175 30.3
-
 D-xylose 25°C, pH 7.0 Caulobacter vibrioides
1.1.1.175 31
-
 NAD+ 25°C, pH 9.0 Caulobacter vibrioides
1.1.1.175 35.7
-
 NAD+ 25°C, pH 7.0 Caulobacter vibrioides

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.175 NAD+
-
 Caulobacter vibrioides

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.175 0.12
-
 L-arabinose 25°C, pH 6.8 Caulobacter vibrioides
1.1.1.175 0.28
-
 L-arabinose 25°C, pH 7.0 Caulobacter vibrioides
1.1.1.175 0.6
-
 L-arabinose 25°C, pH 9.0 Caulobacter vibrioides
1.1.1.175 66.7
-
 D-xylose 25°C, pH 6.8 Caulobacter vibrioides
1.1.1.175 66.7
-
 NAD+ 25°C, pH 9.0 Caulobacter vibrioides
1.1.1.175 101.7
-
 NAD+ 25°C, pH 6.8 Caulobacter vibrioides
1.1.1.175 115
-
 NAD+ 25°C, pH 7.0 Caulobacter vibrioides
1.1.1.175 125
-
 D-xylose 25°C, pH 7.0 Caulobacter vibrioides
1.1.1.175 280
-
 D-xylose 25°C, pH 9.0 Caulobacter vibrioides