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Literature summary extracted from

  • Yang, R.; Chen, H.; Han, Y.; Gu, Z.
    Purification of diamine oxidase and its properties in germinated fava bean (Vicia faba L.) (2012), J. Sci. Food Agric., 92, 1709-1715.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.22 2-mercaptoethanol 2.94% residual activity at 0.5 mM Vicia faba
1.4.3.22 aminoguanidine 3.92% residual activity at 0.25 mM Vicia faba
1.4.3.22 Cu2+ 7.84% residual activity at 3 mM Vicia faba
1.4.3.22 EDTA-Na2 76.47% residual activity at 1 mM Vicia faba
1.4.3.22 EGTA 71.57% residual activity at 1 mM Vicia faba
1.4.3.22 Fe3+ 10.78% residual activity at 3 mM Vicia faba
1.4.3.22 L-cysteine complete inhibition at 0.1 mM Vicia faba
1.4.3.22 Mg2+ 78.43% residual activity at 3 mM Vicia faba

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.22 0.23
-
 putrescine at pH 6.5 and 40°C Vicia faba
1.4.3.22 0.96
-
 spermidine at pH 6.5 and 40°C Vicia faba

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.4.3.22 additional information not influenced by Ca2+, Zn2+, Mn2+, and Ba2+ Vicia faba

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.22 52000
-
 2 * 52000, SDS-PAGE Vicia faba
1.4.3.22 121000
-
 gel filtration Vicia faba

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.22 Vicia faba
-
 cultivar Qidou 2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.22 ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and Sephadex G-100 gel filtration Vicia faba

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.22 seed
-
 Vicia faba
-
1.4.3.22 shoot
-
 Vicia faba
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.22 additional information
-
 the crude extract has a specific activity of 9.1 units/mg, while the 215fold purified enzyme has a specific activity of 1962.47 units/mg. A change in absorbance at 555 nm of 0.01 per minute is considered as one unit of enzyme activity Vicia faba

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.22 additional information the enzyme does not degrade spermine Vicia faba ?
-
 ?
1.4.3.22 putrescine + H2O + O2
-
 Vicia faba ?
-
 ?
1.4.3.22 spermidine + H2O + O2
-
 Vicia faba ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.22 homodimer 2 * 52000, SDS-PAGE Vicia faba

Synonyms

EC Number Synonyms Comment Organism
1.4.3.22 DAO
-
 Vicia faba
1.4.3.22 EC 1.4.3.6 formerly Vicia faba

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.3.22 40
-
-
 Vicia faba

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.3.22 30 60 the enzyme retains its activity after being incubated at 30°C for 30 min, the enzyme is rapidly deactivated above 45°C and reaches almost zero activity at 60°C Vicia faba

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.22 6.5
-
-
 Vicia faba

Expression

EC Number Organism Comment Expression
1.4.3.22 Vicia faba enzyme activity in the shoot is undetectable up to 2 days of germination but then increases significantly up

General Information

EC Number General Information Comment Organism
1.4.3.22 metabolism diamine oxidase is one of the key enzymes for gamma-aminobutyric acid formation Vicia faba