Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Guevara-Flores, A.; Del Arenal, I.P.; Mendoza-Hernandez, G.; Pardo, J.P.; Flores-Herrera, O.; Rendon, J.L.
    Mitochondrial thioredoxin-glutathione reductase from larval Taenia crassiceps (Cysticerci) (2010), J. Parasitol. Res., 2010, 2010.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.B1 auranofin complete inhibition at 0.7 nM Taenia crassiceps

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.B1 0.00087
-
 thioredoxin disulfide at pH 7.8 and 25°C Taenia crassiceps
1.8.1.B1 0.019
-
 NADPH at pH 7.8 and 25°C Taenia crassiceps
1.8.1.B1 0.0345
-
 5,5'-dithiobis(2-nitrobenzoic acid) at pH 7.8 and 25°C Taenia crassiceps
1.8.1.B1 0.041
-
 GSSG at pH 7.8 and 25°C Taenia crassiceps

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.1.B1 mitochondrion
-
 Taenia crassiceps 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.B1 65000
-
 x * 65000, SDS-PAGE Taenia crassiceps

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.1.B1 GSSG + NADPH + H+ Taenia crassiceps
-
 GSH + NADP+
-
 ?
1.8.1.B1 thioredoxin disulfide + NADPH + H+ Taenia crassiceps
-
 thioredoxin + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.B1 Taenia crassiceps
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.B1 DEAE-Sephacel gel filtration, hydroxyapatite chromatography column chromatography, and Cibacron blue chromatography column chromatography Taenia crassiceps

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.1.B1 larva
-
 Taenia crassiceps
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.B1 11.7
-
 crude extract, using GSSG as substrate, at pH 7.8 and 25°C Taenia crassiceps
1.8.1.B1 35
-
 crude extract, using 5,5'-dithiobis(2-nitrobenzoic acid) as substrate, at pH 7.8 and 25°C Taenia crassiceps
1.8.1.B1 128.5
-
 crude extract, using hydroxyethyl disulfide as substrate, at pH 7.8 and 25°C Taenia crassiceps
1.8.1.B1 229
-
 after 31fold purification, using GSSG as substrate, at pH 7.8 and 25°C Taenia crassiceps
1.8.1.B1 1091
-
 after 31fold purification, using 5,5'-dithiobis(2-nitrobenzoic acid) as substrate, at pH 7.8 and 25°C Taenia crassiceps
1.8.1.B1 2243
-
 after 31fold purification, using hydroxyethyl disulfide as substrate, at pH 7.8 and 25°C Taenia crassiceps

Storage Stability

EC Number Storage Stability Organism
1.8.1.B1 -20°C, purified enzyme, 15 days, remains stable Taenia crassiceps

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.B1 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+
-
 Taenia crassiceps 2-nitro-5-thiobenzoate + NADP+
-
 ?
1.8.1.B1 GSSG + NADPH + H+
-
 Taenia crassiceps GSH + NADP+
-
 ?
1.8.1.B1 hydroxyethyl disulfide + NADPH + H+
-
 Taenia crassiceps ?
-
 ?
1.8.1.B1 additional information low catalytic efficiency with H2O2 Taenia crassiceps ?
-
 ?
1.8.1.B1 thioredoxin disulfide + NADPH + H+
-
 Taenia crassiceps thioredoxin + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.1.B1 ? x * 65000, SDS-PAGE Taenia crassiceps

Synonyms

EC Number Synonyms Comment Organism
1.8.1.B1 TGR
-
 Taenia crassiceps
1.8.1.B1 thioredoxin-glutathione reductase
-
 Taenia crassiceps

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.1.B1 28
-
 for 5,5'-dithiobis(2-nitrobenzoic acid) reductase activity Taenia crassiceps
1.8.1.B1 40
-
 for glutathione reductase activity Taenia crassiceps

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.1.B1 0.3
-
 5,5'-dithiobis(2-nitrobenzoic acid) at pH 7.8 and 25°C Taenia crassiceps
1.8.1.B1 0.47
-
 thioredoxin disulfide at pH 7.8 and 25°C Taenia crassiceps
1.8.1.B1 0.84
-
 GSSG at pH 7.8 and 25°C Taenia crassiceps

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.1.B1 7.2
-
 for 5,5'-dithiobis(2-nitrobenzoic acid) reductase activity Taenia crassiceps
1.8.1.B1 7.8
-
 for glutathione reductase activity Taenia crassiceps

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.B1 FAD
-
 Taenia crassiceps
1.8.1.B1 NADPH
-
 Taenia crassiceps

General Information

EC Number General Information Comment Organism
1.8.1.B1 physiological function the enzyme is able to protect glutamine synthetase from oxidative inactivation, suggesting that the enzyme is competent to contend with oxidative stress Taenia crassiceps

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.8.1.B1 8.7
-
 5,5'-dithiobis(2-nitrobenzoic acid) at pH 7.8 and 25°C Taenia crassiceps
1.8.1.B1 22
-
 GSSG at pH 7.8 and 25°C Taenia crassiceps
1.8.1.B1 540
-
 thioredoxin disulfide at pH 7.8 and 25°C Taenia crassiceps