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Literature summary extracted from

  • Hupert-Kocurek, K.; Stawicka, A.; Wojcieszynska, D.; Guzik, U.
    Cloning and mutagenesis of catechol 2,3-dioxygenase Gene from the Gram-positive Planococcus sp. strain S5 (2013), J. Mol. Microbiol. Biotechnol., 23, 381-390.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.2 gene c23o, DNA and amino acid sequence determination and analysis of wild-type and mutant enzymes and recombinant expression in Escherichia coli strain BL21 Planococcus sp. (in: Bacteria)

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.2 A23T/F212S random mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme Planococcus sp. (in: Bacteria)
1.13.11.2 C103R/K289stop random mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme Planococcus sp. (in: Bacteria)
1.13.11.2 F191I/C268R/Y272H/V280A/Y293D / random mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme Planococcus sp. (in: Bacteria)
1.13.11.2 G127D/P140T random mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme Planococcus sp. (in: Bacteria)
1.13.11.2 H24R/F168S/Q275R random mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme Planococcus sp. (in: Bacteria)
1.13.11.2 L13R random mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme Planococcus sp. (in: Bacteria)
1.13.11.2 M65T random mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme Planococcus sp. (in: Bacteria)
1.13.11.2 R296Q random mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme Planococcus sp. (in: Bacteria)
1.13.11.2 R296Q random mutagenesis, the mutant shows significant tolerance to acidic pH with an optimum at pH 5.0, as well as a over 1.5fold increased activity, altered substrate inhibition, and 2.5fold lower KM compared to the wild-type enzyme Planococcus sp. (in: Bacteria)

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.2 0.037
-
 catechol pH 5.0, 35°C, mutant R296Q Planococcus sp. (in: Bacteria)
1.13.11.2 0.04
-
 catechol pH 7.0, 35°C, mutant A23T/F212S Planococcus sp. (in: Bacteria)
1.13.11.2 0.053
-
 catechol pH 7.0, 35°C, mutant H24R/F168S/Q275R Planococcus sp. (in: Bacteria)
1.13.11.2 0.09
-
 catechol pH 7.0, 35°C, wild-type enzyme Planococcus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.2 catechol + O2 Planococcus sp. (in: Bacteria)
-
 2-hydroxymuconate-6-semialdehyde
-
 ?
1.13.11.2 catechol + O2 Planococcus sp. (in: Bacteria) S5
-
 2-hydroxymuconate-6-semialdehyde
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.2 Planococcus sp. (in: Bacteria)
-
 gene c23o
-
1.13.11.2 Planococcus sp. (in: Bacteria) S5
-
 gene c23o
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.2 0.00887
-
 pH 7.0, 35°C, mutant H24R/F168S/Q275R Planococcus sp. (in: Bacteria)
1.13.11.2 0.01285
-
 pH 7.0, 35°C, mutant A23T/F212S Planococcus sp. (in: Bacteria)
1.13.11.2 0.01973
-
 pH 7.0, 35°C, wild-type enzyme Planococcus sp. (in: Bacteria)
1.13.11.2 0.03187
-
 pH 5.0, 35°C, mutant R296Q Planococcus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.2 catechol + O2
-
 Planococcus sp. (in: Bacteria) 2-hydroxymuconate-6-semialdehyde
-
 ?
1.13.11.2 catechol + O2
-
 Planococcus sp. (in: Bacteria) S5 2-hydroxymuconate-6-semialdehyde
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.2 C23o
-
 Planococcus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.2 35
-
 wild-type and mutant enzymes Planococcus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.2 5
-
 mutant R296Q Planococcus sp. (in: Bacteria)
1.13.11.2 6.5
-
 mutants A23T/F212S and H24R/F168S/Q275R Planococcus sp. (in: Bacteria)
1.13.11.2 7
-
 wild-type enzyme Planococcus sp. (in: Bacteria)

General Information

EC Number General Information Comment Organism
1.13.11.2 additional information the enzyme is a member of the superfamily I, subfamily 2A of extradiol dioxygenases Planococcus sp. (in: Bacteria)
1.13.11.2 physiological function catechol 2,3-dioxygenases play a role in the degradation of monoaromatic hydrocarbons Planococcus sp. (in: Bacteria)