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Literature summary extracted from

  • Wang, P.; Yang, J.; Jiang, L.; Feng, J.; Yang, C.; Li, D.
    A bi-enzymatic system for efficient enantioselective bioconversion of racemic mandelic acid (2013), J. Mol. Catal. B, 94, 47-50.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.99.31 expressed in Escherichia coli BL21(DE3) cells Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.31 0.92
-
(S)-Mandelate at pH 6.5 and 30°C Pseudomonas aeruginosa
1.1.99.31 3.87
-
ferricyanide at pH 6.5 and 30°C Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.99.31 (S)-mandelate + 2 ferricyanide Pseudomonas aeruginosa
-
benzoylformic acid + 2 ferrocyanide + 2 H+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.31 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.31 Toyopearl DEAE-650M column chromatography Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.99.31 2.68
-
crude extract, at pH 6.5 and 30°C Pseudomonas aeruginosa
1.1.99.31 20.5
-
after 7.65fold purification, at pH 6.5 and 30°C Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.31 (S)-mandelate + 2 ferricyanide
-
Pseudomonas aeruginosa benzoylformic acid + 2 ferrocyanide + 2 H+
-
?

Synonyms

EC Number Synonyms Comment Organism
1.1.99.31 SMDH
-
Pseudomonas aeruginosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.99.31 30
-
-
Pseudomonas aeruginosa

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.99.31 20 40 about 58% activity at 20°C, about 50% activity at 40°C Pseudomonas aeruginosa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.99.31 30
-
the remaining activity after treatment in 50 mM potassium phosphate buffer (pH 6.5) at 30°C was 92% for 15 h Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.99.31 6.5
-
-
Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.31 FMN
-
Pseudomonas aeruginosa