EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.99.31 | expressed in Escherichia coli BL21(DE3) cells | Pseudomonas aeruginosa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.99.31 | 0.92 | - |
(S)-Mandelate | at pH 6.5 and 30°C | Pseudomonas aeruginosa | |
1.1.99.31 | 3.87 | - |
ferricyanide | at pH 6.5 and 30°C | Pseudomonas aeruginosa |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.99.31 | (S)-mandelate + 2 ferricyanide | Pseudomonas aeruginosa | - |
benzoylformic acid + 2 ferrocyanide + 2 H+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.99.31 | Pseudomonas aeruginosa | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.99.31 | Toyopearl DEAE-650M column chromatography | Pseudomonas aeruginosa |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.99.31 | 2.68 | - |
crude extract, at pH 6.5 and 30°C | Pseudomonas aeruginosa |
1.1.99.31 | 20.5 | - |
after 7.65fold purification, at pH 6.5 and 30°C | Pseudomonas aeruginosa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.99.31 | (S)-mandelate + 2 ferricyanide | - |
Pseudomonas aeruginosa | benzoylformic acid + 2 ferrocyanide + 2 H+ | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.99.31 | SMDH | - |
Pseudomonas aeruginosa |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.99.31 | 30 | - |
- |
Pseudomonas aeruginosa |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.99.31 | 20 | 40 | about 58% activity at 20°C, about 50% activity at 40°C | Pseudomonas aeruginosa |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.99.31 | 30 | - |
the remaining activity after treatment in 50 mM potassium phosphate buffer (pH 6.5) at 30°C was 92% for 15 h | Pseudomonas aeruginosa |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.99.31 | 6.5 | - |
- |
Pseudomonas aeruginosa |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.99.31 | FMN | - |
Pseudomonas aeruginosa |