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Literature summary extracted from
- Characterization and application of a glycolate dehydrogenase from Trichoderma harzianum AIU 353 (2012), J. Mol. Catal. B, 83, 94-99.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.99.14 | 8-oxyquinoline | 80% residual activity at 1 mM | Trichoderma harzianum |  |
| 1.1.99.14 | Co2+ | 88% residual activity at 1 mM | Trichoderma harzianum | |
| 1.1.99.14 | Cu2+ | complete inhibition at 1 mM | Trichoderma harzianum | |
| 1.1.99.14 | DW 1750000 | 91% residual activity at 1 mM | Trichoderma harzianum | |
| 1.1.99.14 | Fe3+ | 73% residual activity at 1 mM | Trichoderma harzianum | |
| 1.1.99.14 | glyoxylate | 79% residual activity at 1 mM | Trichoderma harzianum | |
| 1.1.99.14 | Hg2+ | 2% residual activity at 1 mM | Trichoderma harzianum | |
| 1.1.99.14 | hydrazine | 77% residual activity at 1 mM | Trichoderma harzianum | |
| 1.1.99.14 | hydroxylamine | 83% residual activity at 1 mM | Trichoderma harzianum | |
| 1.1.99.14 | iodoacetic acid | 63% residual activity at 1 mM | Trichoderma harzianum | |
| 1.1.99.14 | Mn2+ | complete inhibition at 1 mM | Trichoderma harzianum | |
| 1.1.99.14 | additional information | not inhibited by semicarbazide, 4-chloromercuribenzoic acid and EDTA | Trichoderma harzianum | |
| 1.1.99.14 | N-ethylmaleimide | 56% residual activity at 1 mM | Trichoderma harzianum | |
| 1.1.99.14 | Ni2+ | complete inhibition at 1 mM | Trichoderma harzianum | |
| 1.1.99.14 | o-phenanthroline | 88% residual activity at 1 mM | Trichoderma harzianum | |
| 1.1.99.14 | Sodium azide | 85% residual activity at 1 mM | Trichoderma harzianum | |
| 1.1.99.14 | Zn2+ | 76% residual activity at 1 mM | Trichoderma harzianum | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.99.14 | 0.15 | - |
Nitro blue tetrazolium | at pH 7.0 and 30°C | Trichoderma harzianum | |
| 1.1.99.14 | 0.94 | - |
glycolate | at pH 7.0 and 30°C | Trichoderma harzianum | |
| 1.1.99.14 | 3.6 | - |
L-lactate | at pH 7.0 and 30°C | Trichoderma harzianum | |
| 1.1.99.14 | 49.5 | - |
dihydroxyacetone | at pH 7.0 and 30°C | Trichoderma harzianum | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.14 | 38000 | - |
1 * 107000 + 1 * 81000 + 1 * 53000 + 1 * 38000, SDS-PAGE | Trichoderma harzianum |
| 1.1.99.14 | 53000 | - |
1 * 107000 + 1 * 81000 + 1 * 53000 + 1 * 38000, SDS-PAGE | Trichoderma harzianum |
| 1.1.99.14 | 81000 | - |
1 * 107000 + 1 * 81000 + 1 * 53000 + 1 * 38000, SDS-PAGE | Trichoderma harzianum |
| 1.1.99.14 | 107000 | - |
1 * 107000 + 1 * 81000 + 1 * 53000 + 1 * 38000, SDS-PAGE | Trichoderma harzianum |
| 1.1.99.14 | 280000 | - |
gel filtration | Trichoderma harzianum |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.99.14 | additional information | Trichoderma harzianum | the enzyme does not require NAD+ and NADP+ as an electron carrier, and primary alcohols, dihydric alcohols and aliphatic aldehydes are not oxidized. Ethanol, 1-propanol, 2-propanol, 1-butanol, ethylene glycol, propylene glycol, 1,3-propanediol, glycerol, glyoxylate, glyoxal, methylglyoxal, acetaldehyde, propylaldehyde, L-malic acid, L-kactic acid, D-malic acid and L-tartaric acid are inert | ? | - |
? | |
| 1.1.99.14 | additional information | Trichoderma harzianum AIU 353 | the enzyme does not require NAD+ and NADP+ as an electron carrier, and primary alcohols, dihydric alcohols and aliphatic aldehydes are not oxidized. Ethanol, 1-propanol, 2-propanol, 1-butanol, ethylene glycol, propylene glycol, 1,3-propanediol, glycerol, glyoxylate, glyoxal, methylglyoxal, acetaldehyde, propylaldehyde, L-malic acid, L-kactic acid, D-malic acid and L-tartaric acid are inert | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.99.14 | Trichoderma harzianum | - |
- |
- |
| 1.1.99.14 | Trichoderma harzianum AIU 353 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.99.14 | ammonium sulfate precipitation, DEAE-Toyopearl column chromatography, Q-Toyopearl column chromatography, and Toyopearl, GigaCap Q-Toyopearl column chromatography, hydroxyapatite column chromatography, and gel filtration | Trichoderma harzianum |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.14 | 0.00165 | - |
crude cell extract, at pH 7.0 and 30°C | Trichoderma harzianum |
| 1.1.99.14 | 0.727 | - |
after 440fold purification, at pH 7.0 and 30°C | Trichoderma harzianum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.99.14 | dihydroxyacetone + nitro blue tetrazolium | 162% activity compared to glycolate | Trichoderma harzianum | ? + reduced nitro blue tetrazolium | - |
? | |
| 1.1.99.14 | dihydroxyacetone + nitro blue tetrazolium | 162% activity compared to glycolate | Trichoderma harzianum AIU 353 | ? + reduced nitro blue tetrazolium | - |
? | |
| 1.1.99.14 | DL-glyceraldehyde + nitro blue tetrazolium | 78% activity compared to glycolate | Trichoderma harzianum | ? + reduced nitro blue tetrazolium | - |
? | |
| 1.1.99.14 | DL-glyceraldehyde + nitro blue tetrazolium | 78% activity compared to glycolate | Trichoderma harzianum AIU 353 | ? + reduced nitro blue tetrazolium | - |
? | |
| 1.1.99.14 | DL-lactate + nitro blue tetrazolium | 235% activity compared to glycolate | Trichoderma harzianum | ? + reduced nitro blue tetrazolium | - |
? | |
| 1.1.99.14 | DL-lactate + nitro blue tetrazolium | 235% activity compared to glycolate | Trichoderma harzianum AIU 353 | ? + reduced nitro blue tetrazolium | - |
? | |
| 1.1.99.14 | glycolaldehyde + nitro blue tetrazolium | 38% activity compared to glycolate | Trichoderma harzianum | ? + reduced nitro blue tetrazolium | - |
? | |
| 1.1.99.14 | glycolate + 2,6-dichlorophenolindophenol | - |
Trichoderma harzianum | glyoxylate + reduced 2,6-dichlorophenolindophenol | - |
? | |
| 1.1.99.14 | glycolate + 2,6-dichlorophenolindophenol | - |
Trichoderma harzianum AIU 353 | glyoxylate + reduced 2,6-dichlorophenolindophenol | - |
? | |
| 1.1.99.14 | glycolate + nitro blue tetrazolium | 100% activity | Trichoderma harzianum | glyoxylate + reduced nitro blue tetrazolium | - |
? | |
| 1.1.99.14 | L-lactate + nitro blue tetrazolium | 232% activity compared to glycolate | Trichoderma harzianum | ? + reduced nitro blue tetrazolium | - |
? | |
| 1.1.99.14 | methyl glycolate + nitro blue tetrazolium | 82% activity compared to glycolate | Trichoderma harzianum | methyl glyoxylate + reduced nitro blue tetrazolium | - |
? | |
| 1.1.99.14 | additional information | the enzyme does not require NAD+ and NADP+ as an electron carrier, and primary alcohols, dihydric alcohols and aliphatic aldehydes are not oxidized. Ethanol, 1-propanol, 2-propanol, 1-butanol, ethylene glycol, propylene glycol, 1,3-propanediol, glycerol, glyoxylate, glyoxal, methylglyoxal, acetaldehyde, propylaldehyde, L-malic acid, L-kactic acid, D-malic acid and L-tartaric acid are inert | Trichoderma harzianum | ? | - |
? | |
| 1.1.99.14 | additional information | the enzyme does not require NAD+ and NADP+ as an electron carrier, and primary alcohols, dihydric alcohols and aliphatic aldehydes are not oxidized. Ethanol, 1-propanol, 2-propanol, 1-butanol, ethylene glycol, propylene glycol, 1,3-propanediol, glycerol, glyoxylate, glyoxal, methylglyoxal, acetaldehyde, propylaldehyde, L-malic acid, L-kactic acid, D-malic acid and L-tartaric acid are inert | Trichoderma harzianum AIU 353 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.99.14 | heterotetramer | 1 * 107000 + 1 * 81000 + 1 * 53000 + 1 * 38000, SDS-PAGE | Trichoderma harzianum |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.99.14 | GADH | - |
Trichoderma harzianum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.14 | 30 | - |
- |
Trichoderma harzianum |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.14 | 20 | 60 | when the enzyme is incubated with 0.1 M potassium phosphate buffer, pH 6.0, at 2060°C for 30 min, approximately 90% of the enzyme activity remain below 20°C, but the activity completely disappears at 40°C | Trichoderma harzianum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.14 | 7 | - |
- |
Trichoderma harzianum |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.14 | 5 | 6.5 | more than 80% of enzyme activity remains in the pH range between 5.0 and 6.5 for 20 min at 20°C | Trichoderma harzianum |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 1.1.99.14 | Trichoderma harzianum | isoelectric focusing | - |
5.1 |
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