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Literature summary extracted from

  • Zhao, Y.; Wakamatsu, T.; Doi, K.; Sakuraba, H.; Ohshima, T.
    A psychrophilic leucine dehydrogenase from Sporosarcina psychrophila: purification, characterization, gene sequencing and crystal structure analysis (2012), J. Mol. Catal. B, 83, 65-72.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.9 0.015
-
 NAD+ pH 10.5, 20°C Sporosarcina psychrophila
1.4.1.9 0.65
-
 L-leucine pH 10.5, 20°C Sporosarcina psychrophila

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.9 43000
-
 8 * 43000, SDS-PAGE Sporosarcina psychrophila
1.4.1.9 340000
-
 gel filtration Sporosarcina psychrophila

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.9 L-leucine + H2O + NAD+ Sporosarcina psychrophila
-
 4-methyl-2-oxopentanoate + NH3 + NADH + H+
-
 ?
1.4.1.9 L-leucine + H2O + NAD+ Sporosarcina psychrophila DSM 3
-
 4-methyl-2-oxopentanoate + NH3 + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.9 Sporosarcina psychrophila
-
-
-
1.4.1.9 Sporosarcina psychrophila DSM 3
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.9 native enzyme 48fold by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography , and gel filtration to over 95% purity Sporosarcina psychrophila

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.9 0.074
-
 strain DSM 3, pH 10.5, 25°C Sporosarcina psychrophila
1.4.1.9 53.8
-
 purified enzyme, pH 10.5, 25°C Sporosarcina psychrophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.9 L-leucine + H2O + NAD+
-
 Sporosarcina psychrophila 4-methyl-2-oxopentanoate + NH3 + NADH + H+
-
 ?
1.4.1.9 L-leucine + H2O + NAD+
-
 Sporosarcina psychrophila DSM 3 4-methyl-2-oxopentanoate + NH3 + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.1.9 homooctamer 8 * 43000, SDS-PAGE Sporosarcina psychrophila

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.9 50
-
-
 Sporosarcina psychrophila

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.4.1.9
-
 50 highest activity at 50°C, 10% of maximal activity at 0°C Sporosarcina psychrophila

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.9 25
-
 purified enzyme, fully stable at pH 5.0-11.0 Sporosarcina psychrophila
1.4.1.9 40
-
 purified enzyme, 40 min, fully stable Sporosarcina psychrophila
1.4.1.9 50
-
 purified enzyme, half-life is 30 min Sporosarcina psychrophila
1.4.1.9 55
-
 purified enzyme, half-life is 10 min Sporosarcina psychrophila

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.1.9 10
-
 L-leucine pH 10.5, 20°C Sporosarcina psychrophila

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.9 9
-
 amination of 4-methyl-2-oxopentanoate Sporosarcina psychrophila
1.4.1.9 11
-
 deamination of l-leucine Sporosarcina psychrophila

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.1.9 5 11 purified enzyme, fully stable at 25°C Sporosarcina psychrophila

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.9 NAD+
-
 Sporosarcina psychrophila
1.4.1.9 NADH
-
 Sporosarcina psychrophila