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Literature summary extracted from

  • Ben Younes, S.; Sayadi, S.
    Purification and characterization of a novel trimeric and thermotolerant laccase produced from the ascomycete Scytalidium thermophilum strain (2011), J. Mol. Catal. B, 73, 35-42.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 DTT
-
 Mycothermus thermophilus
1.10.3.2 EDTA
-
 Mycothermus thermophilus
1.10.3.2 Fe2+ inhibits laccase activity completely at 10 mM Mycothermus thermophilus
1.10.3.2 additional information poor inhibition by NaF and NaBr Mycothermus thermophilus
1.10.3.2 NaN3
-
 Mycothermus thermophilus
1.10.3.2 SDS
-
 Mycothermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 additional information
-
 additional information steady-state kinetics, overview Mycothermus thermophilus
1.10.3.2 0.026
-
 2,6-dimethoxyphenol pH 5.0, 22-25°C Mycothermus thermophilus
1.10.3.2 0.036
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 5.0, 22-25°C Mycothermus thermophilus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.10.3.2 extracellular
-
 Mycothermus thermophilus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 additional information laccase activity remains stable for 24 h against all the ions tested, i.e. Cu2+, Co2+, Pb2+, Ca2+, Cd2+, Mg2+, Mn2+, Fe2+, Ni2+, F-, Zn2+, and Li+, at 10-100 mM Mycothermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 82000
-
 gel filtration Mycothermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Mycothermus thermophilus FJ560721 isolated from locally prepared compost in the north of Tunisia
-
1.10.3.2 Mycothermus thermophilus ST26 FJ560721 isolated from locally prepared compost in the north of Tunisia
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2 native enzyme 8fold by two teps of anion exchange chromatography, and gel filtration to homogeneity Mycothermus thermophilus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.2 mycelium
-
 Mycothermus thermophilus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.2 139.4
-
 purified native enzyme, pH 5.0, 22-25°C Mycothermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Mycothermus thermophilus ?
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Mycothermus thermophilus ?
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Mycothermus thermophilus ST26 ?
-
 ?
1.10.3.2 4 catechol + O2
-
 Mycothermus thermophilus 4 1,2-benzoquinone + 2 H2O
-
 ?
1.10.3.2 guaiacol + O2
-
 Mycothermus thermophilus ?
-
 ?
1.10.3.2 guaiacol + O2
-
 Mycothermus thermophilus ST26 ?
-
 ?
1.10.3.2 hydroquinone + O2
-
 Mycothermus thermophilus ?
-
 ?
1.10.3.2 hydroquinone + O2
-
 Mycothermus thermophilus ST26 ?
-
 ?
1.10.3.2 additional information substrate specificity of purified enzyme, overview. No activity with ferulic acid Mycothermus thermophilus ?
-
 ?
1.10.3.2 additional information substrate specificity of purified enzyme, overview. No activity with ferulic acid Mycothermus thermophilus ST26 ?
-
 ?
1.10.3.2 pyrogallol + O2
-
 Mycothermus thermophilus ?
-
 ?
1.10.3.2 pyrogallol + O2
-
 Mycothermus thermophilus ST26 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 homotrimer 3 x 28000, SDS-PAGE Mycothermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 70
-
 with substrate 2,6-dimethoxyphenol Mycothermus thermophilus
1.10.3.2 80
-
 with substrate 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) Mycothermus thermophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.10.3.2 65 85 activity range Mycothermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 70
-
 the purified native enzyme retains 50% of its activity after 120 min of incubation Mycothermus thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.10.3.2 0.14
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 5.0, 22-25°C Mycothermus thermophilus
1.10.3.2 0.35
-
 2,6-dimethoxyphenol pH 5.0, 22-25°C Mycothermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 5
-
 with substrates 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) and 2,6-dimethoxyphenol Mycothermus thermophilus
1.10.3.2 6
-
 with substrates guaiacol and hydroquinone Mycothermus thermophilus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.10.3.2 1.375
-
 2,6-dimethoxyphenol pH 5.0, 22-25°C Mycothermus thermophilus
1.10.3.2 5.05
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 5.0, 22-25°C Mycothermus thermophilus