EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.5.1.54 | hanging-drop vapor diffusion, ctystal structure of wild-type enzyme and mutant enzyme I181D | Pyrococcus furiosus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.5.1.54 | I181D | mutant enzyme is catalytically more active than the wild type enzyme from 20 to 80°C, the mutation disrupts the tetrameric structure of the enzyme, the melting temperatures of the wild-type protein are significantly higher than the melting temperatures of mutant enzyme I181D at pH values greater than 6.5 | Pyrococcus furiosus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.54 | 0.066 | - |
phosphoenolpyruvate | pH 7.5, 60°C, mutant enzyme I181D | Pyrococcus furiosus | |
2.5.1.54 | 0.071 | - |
D-erythrose 4-phosphate | pH 7.5, 60°C, mutant enzyme I181D | Pyrococcus furiosus | |
2.5.1.54 | 0.092 | - |
D-erythrose 4-phosphate | pH 7.5, 60°C, wild-type enzyme | Pyrococcus furiosus | |
2.5.1.54 | 0.112 | - |
phosphoenolpyruvate | pH 7.5, 60°C, wild-type enzyme | Pyrococcus furiosus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.5.1.54 | 109000 | - |
sedimentation equilibrium experiments, tetrameric enzyme | Pyrococcus furiosus |
2.5.1.54 | 111000 | - |
gel filtration, tetrameric enzyme | Pyrococcus furiosus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.54 | Pyrococcus furiosus | Q8U0A9 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.54 | phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Pyrococcus furiosus | 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.5.1.54 | dimer | although the enzyme crystallizes as a tetramer, equilibrium exists between tetrameric and dimeric forms with a dissociation constant of 0.022 mM | Pyrococcus furiosus |
2.5.1.54 | tetramer | at pH 7.5 and 20°C, the wild-type enzyme is present as a tetramer in solution. Although the enzyme crystallizes as a tetramer, equilibrium exists between tetrameric and dimeric forms with a dissociation constant of 0.022 mM | Pyrococcus furiosus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.54 | 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase | - |
Pyrococcus furiosus |
2.5.1.54 | DAH7PS | - |
Pyrococcus furiosus |
2.5.1.54 | PF1690 | locus name | Pyrococcus furiosus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.5.1.54 | 60 | - |
assay at | Pyrococcus furiosus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.5.1.54 | 98 | - |
melting temperature of the wild-type enzyme by differential scanning calorimetry at a protein monomer concentration of 0.034 mM and a pH of 7.5 | Pyrococcus furiosus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.54 | 5.5 | - |
phosphoenolpyruvate | pH 7.5, 60°C, wild-type enzyme | Pyrococcus furiosus | |
2.5.1.54 | 5.5 | - |
D-erythrose 4-phosphate | pH 7.5, 60°C, wild-type enzyme | Pyrococcus furiosus | |
2.5.1.54 | 14.9 | - |
phosphoenolpyruvate | pH 7.5, 60°C, mutant enzyme I181D | Pyrococcus furiosus | |
2.5.1.54 | 14.9 | - |
D-erythrose 4-phosphate | pH 7.5, 60°C, mutant enzyme I181D | Pyrococcus furiosus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.54 | 7.5 | - |
assay at | Pyrococcus furiosus |
2.5.1.54 | 7.5 | - |
mutant enzyme I181D | Pyrococcus furiosus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.54 | 6 | 9 | at pH 9.0 the activity is about 60% of the activity at pH 6.0, wid-type enzyme | Pyrococcus furiosus |
2.5.1.54 | 6 | 9 | pH 6.0: about 55% of maximal activity, pH 9.0: about 35% of maximal activity, mutant enzyme I181D | Pyrococcus furiosus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.5.1.54 | physiological function | the enzyme is involved in shikimate biosynthesis pathway | Pyrococcus furiosus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.54 | 0.21 | - |
D-erythrose 4-phosphate | pH 7.5, 60°C, mutant enzyme I181D | Pyrococcus furiosus | |
2.5.1.54 | 0.22 | - |
phosphoenolpyruvate | pH 7.5, 60°C, mutant enzyme I181D | Pyrococcus furiosus | |
2.5.1.54 | 49 | - |
phosphoenolpyruvate | pH 7.5, 60°C, wild-type enzyme | Pyrococcus furiosus | |
2.5.1.54 | 60 | - |
D-erythrose 4-phosphate | pH 7.5, 60°C, wild-type enzyme | Pyrococcus furiosus |