Literature summary extracted from

D'Ambrosio, K.; Pedone, E.; Langella, E.; De Simone, G.; Rossi, M.; Pedone, C.; Bartolucci, S.
A novel member of the protein disulfide oxidoreductase family from Aeropyrum pernix K1: structure, function and electrostatics (2006), J. Mol. Biol., 362, 743-752.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.8.99.B1	expression in Escherichia coli	Aeropyrum pernix

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
1.8.99.B1	crystals are grown in the presence of 2 M ammonium sulfate, 2% (v/v) PEG 400, 0.1 M Hepes (pH 8). X-ray diffraction data are collected to 1.93 A resolution	Aeropyrum pernix

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
1.8.99.B1	27198	-	1 * 27198, calculated from sequence, gel filtration, electrospray mass spectroscopy	Aeropyrum pernix
1.8.99.B1	27200	-	gel filtration, electrospray mass spectroscopy	Aeropyrum pernix

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.8.99.B1	Aeropyrum pernix	Q9YDZ4	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.8.99.B1	-	Aeropyrum pernix

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.8.99.B1	additional information	the enzyme reveals an inherent glutathione-dependent thioltransferase activity	Aeropyrum pernix	?	-	?
1.8.99.B1	[insulin]-disulfide + reduced dithiothreitol	-	Aeropyrum pernix	[insulin]-dithiol + oxidized dithiothreitol	-	?

Subunits

EC Number	Subunits	Comment	Organism
1.8.99.B1	monomer	1 * 27198, calculated from sequence, gel filtration, electrospray mass spectroscopy	Aeropyrum pernix

Synonyms

EC Number	Synonyms	Comment	Organism
1.8.99.B1	ApPDO	-	Aeropyrum pernix

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
1.8.99.B1	30	-	assay at	Aeropyrum pernix

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)