Literature summary extracted from
Pedone, E.; D'Ambrosio, K.; De Simone, G.; Rossi, M.; Pedone, C.; Bartolucci, S.
Insights on a new PDI-like family: structural and functional analysis of a protein disulfide oxidoreductase from the bacterium Aquifex aeolicus (2005), J. Mol. Biol., 356, 155-164.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.8.99.B1 |
mutant enzymes C34S, C148S and C34S/C148S, are overexpressed in Escherichia coli |
Aquifex aeolicus |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
1.8.99.B1 |
crystal structure at 2.4 A resolution |
Aquifex aeolicus |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
1.8.99.B1 |
C148S |
mutant enzyme exhibits a lower activity compared to wild-type enzyme |
Aquifex aeolicus |
1.8.99.B1 |
C34S |
mutant enzyme retains nearly wild-type activity |
Aquifex aeolicus |
1.8.99.B1 |
C34S/C148S |
mutant enzyme shows no activity |
Aquifex aeolicus |
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
1.8.99.B1 |
26700 |
- |
gel-filtration, electrospray mass spectroscopy |
Aquifex aeolicus |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.8.99.B1 |
Aquifex aeolicus |
O66753 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.8.99.B1 |
wild-type enzyme and recombinant mutant enzymes C34S, C148S and C34S/C148S |
Aquifex aeolicus |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.8.99.B1 |
additional information |
the enzyme reveals an inherent glutathione-dependent thioltransferase activity |
Aquifex aeolicus |
? |
- |
? |
|
1.8.99.B1 |
[insulin]-disulfide + reduced dithiothreitol |
- |
Aquifex aeolicus |
[insulin]-dithiol + oxidized dithiothreitol |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.8.99.B1 |
AaPDO |
- |
Aquifex aeolicus |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
1.8.99.B1 |
30 |
- |
assay at |
Aquifex aeolicus |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
1.8.99.B1 |
7 |
- |
assay at |
Aquifex aeolicus |