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Literature summary extracted from

  • Zou, Y.J.; Wang, H.X.; Ng, T.B.; Huang, C.Y.; Zhang, J.X.
    Purification and characterization of a novel laccase from the edible mushroom Hericium coralloides (2012), J. Microbiol., 50, 72-78.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 Ca2+ inhibits the enzyme activity at 100 mM but increases it at 12.5-50 mM Hericium coralloides
1.10.3.2 Cd2+ inhibits the enzyme activity at 100 mM but increases it at 12.5-50 mM Hericium coralloides
1.10.3.2 Co2+ inhibits the enzyme activity at 100 mM but increases it at 12.5-50 mM Hericium coralloides
1.10.3.2 Fe2+
-
 Hericium coralloides
1.10.3.2 Hg2+
-
 Hericium coralloides
1.10.3.2 K+ inhibits the enzyme activity at 100 mM but increases it at 12.5-50 mM Hericium coralloides
1.10.3.2 Mn2+ inhibits the enzyme activity at 100 mM but increases it at 12.5-50 mM Hericium coralloides
1.10.3.2 Zn2+ 22% inhibition at 100 mM Hericium coralloides

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 Al3+ activates Hericium coralloides
1.10.3.2 Ca2+ inhibits the enzyme activity at 100 mM but increases it at 12.5-50 mM Hericium coralloides
1.10.3.2 Cd2+ inhibits the enzyme activity at 100 mM but increases it at 12.5-50 mM Hericium coralloides
1.10.3.2 Co2+ inhibits the enzyme activity at 100 mM but increases it at 12.5-50 mM Hericium coralloides
1.10.3.2 K+ inhibits the enzyme activity at 100 mM but increases it at 12.5-50 mM Hericium coralloides
1.10.3.2 Mg2+ activates Hericium coralloides
1.10.3.2 Mn2+ inhibits the enzyme activity at 100 mM but increases it at 12.5-50 mM Hericium coralloides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 65000
-
 native enzyme, gel filtration Hericium coralloides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.10.3.2 4 catechol + O2 Hericium coralloides
-
 4 1,2-benzoquinone + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Hericium coralloides
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2 native enzyme 24.3fold by two different steps of anion exchange chromatography, and gel filtration Hericium coralloides

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.2 fruit body dried Hericium coralloides
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 4 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 best substrate Hericium coralloides ?
-
 ?
1.10.3.2 4 2-methoxyphenol + O2
-
 Hericium coralloides ?
-
 ?
1.10.3.2 4 catechol + O2
-
 Hericium coralloides 4 1,2-benzoquinone + 2 H2O
-
 ?
1.10.3.2 4 N,N-dimethyl-1,4-phenylenediamine + O2
-
 Hericium coralloides ?
-
 ?
1.10.3.2 4 pyrogallol + O2
-
 Hericium coralloides ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 monomer x * 65000, SDS-PAGE Hericium coralloides

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 40
-
-
 Hericium coralloides

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.10.3.2 20 100 24% of maximal activity at 100°C, 65% at 20°C, activity profile, overview Hericium coralloides

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 100
-
 purified native enzyme, 10 min, inactivation Hericium coralloides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 2.2
-
-
 Hericium coralloides

General Information

EC Number General Information Comment Organism
1.10.3.2 additional information the laccase does not exert any antiproliferative activity against Hep-G2 or MCF-7 tumor cell lines at a concentration of 0.06 mM, but shows significant activity toward human immunodeficiency virus-1 reverse transcriptase with an IC50 of 60 nM Hericium coralloides