EC Number | Application | Comment | Organism |
---|---|---|---|
3.2.1.4 | synthesis | glucose production from cellulose material using beta-glucosidase from Pyrococcus furioses and endocellulase from Pyrococcus horikoshii. The combination reaction can produce only glucose without the other oligosaccharides from phosphoric acid swollen Avicel | Pyrococcus horikoshii |
3.2.1.21 | synthesis | glucose production from cellulose material using beta-glucosidase from Pyrococcus furioses and endocellulase from Pyrococcus horikoshii. The combination reaction can produce only glucose without the other oligosaccharides from phosphoric acid swollen Avicel | Pyrococcus furiosus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.4 | cellobiose | the non-complete saccharification of cellulose by thr enzyme seems to be due to product-feedback inhibition by cellobiose | Pyrococcus horikoshii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.21 | 20.3 | - |
cellobiose | pH 5.5, 85°C | Pyrococcus furiosus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.4 | Pyrococcus horikoshii | O58925 | - |
- |
3.2.1.4 | Pyrococcus horikoshii DSM 12428 | O58925 | - |
- |
3.2.1.21 | Pyrococcus furiosus | E7FHY4 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.4 | 4-nitrophenyl cellobioside + H2O | - |
Pyrococcus horikoshii | 4-nitrophenol + cellobiose | - |
? | |
3.2.1.4 | 4-nitrophenyl cellobioside + H2O | - |
Pyrococcus horikoshii DSM 12428 | 4-nitrophenol + cellobiose | - |
? | |
3.2.1.4 | avicel + H2O | the main product is cellobiose | Pyrococcus horikoshii | cellobiose + ? | - |
? | |
3.2.1.4 | avicel + H2O | the main product is cellobiose | Pyrococcus horikoshii DSM 12428 | cellobiose + ? | - |
? | |
3.2.1.21 | cellobiose + H2O | - |
Pyrococcus furiosus | 2 beta-D-glucose | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.4 | EGPh | - |
Pyrococcus horikoshii |
3.2.1.4 | PH1171 | locus name | Pyrococcus horikoshii |
3.2.1.21 | PF0073 | locus name | Pyrococcus furiosus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.4 | 50 | - |
assay at | Pyrococcus horikoshii |
3.2.1.21 | 85 | - |
- |
Pyrococcus furiosus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.21 | 594 | - |
cellobiose | pH 5.5, 85°C | Pyrococcus furiosus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.4 | 5.5 | - |
assay at | Pyrococcus horikoshii |
3.2.1.21 | 5.5 | - |
assay at | Pyrococcus furiosus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.4 | 9.08 | - |
cellobiose | pH 5.5, 50°C | Pyrococcus horikoshii |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.21 | 29.3 | - |
cellobiose | pH 5.5, 85°C | Pyrococcus furiosus |