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Literature summary extracted from

  • Kim, H.W.; Ishikawa, K.
    Complete saccharification of cellulose at high temperature using endocellulase and beta-glucosidase from Pyrococcus sp. (2010), J. Microbiol. Biotechnol., 20, 889-892.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.4 synthesis glucose production from cellulose material using beta-glucosidase from Pyrococcus furioses and endocellulase from Pyrococcus horikoshii. The combination reaction can produce only glucose without the other oligosaccharides from phosphoric acid swollen Avicel Pyrococcus horikoshii
3.2.1.21 synthesis glucose production from cellulose material using beta-glucosidase from Pyrococcus furioses and endocellulase from Pyrococcus horikoshii. The combination reaction can produce only glucose without the other oligosaccharides from phosphoric acid swollen Avicel Pyrococcus furiosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.4 cellobiose the non-complete saccharification of cellulose by thr enzyme seems to be due to product-feedback inhibition by cellobiose Pyrococcus horikoshii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 20.3
-
cellobiose pH 5.5, 85°C Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Pyrococcus horikoshii O58925
-
-
3.2.1.4 Pyrococcus horikoshii DSM 12428 O58925
-
-
3.2.1.21 Pyrococcus furiosus E7FHY4
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 4-nitrophenyl cellobioside + H2O
-
Pyrococcus horikoshii 4-nitrophenol + cellobiose
-
?
3.2.1.4 4-nitrophenyl cellobioside + H2O
-
Pyrococcus horikoshii DSM 12428 4-nitrophenol + cellobiose
-
?
3.2.1.4 avicel + H2O the main product is cellobiose Pyrococcus horikoshii cellobiose + ?
-
?
3.2.1.4 avicel + H2O the main product is cellobiose Pyrococcus horikoshii DSM 12428 cellobiose + ?
-
?
3.2.1.21 cellobiose + H2O
-
Pyrococcus furiosus 2 beta-D-glucose
-
?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 EGPh
-
Pyrococcus horikoshii
3.2.1.4 PH1171 locus name Pyrococcus horikoshii
3.2.1.21 PF0073 locus name Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 50
-
assay at Pyrococcus horikoshii
3.2.1.21 85
-
-
Pyrococcus furiosus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.21 594
-
cellobiose pH 5.5, 85°C Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 5.5
-
assay at Pyrococcus horikoshii
3.2.1.21 5.5
-
assay at Pyrococcus furiosus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.4 9.08
-
cellobiose pH 5.5, 50°C Pyrococcus horikoshii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.21 29.3
-
cellobiose pH 5.5, 85°C Pyrococcus furiosus